Journals / Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi / 2019 / Cilt: 20 - Sayı: 2

Antimicrobial And antioxidant effect of Helvella leucomelaena (Pers.) Nannf.

Helvella leucomelaena (Pers.) Nannf.’ın antimikrobiyal ve antioksidan Etkisi

Pages
249–253
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The scavenging effect of DPPH radical was observed to increase depend to increasing concentrations.

📌 3 Key Takeaways

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Objective & Hypothesis:Mushrooms have been used by the public for many years due to the active containts they contain as well as being consumed as a nutritious food.
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Key Findings & Data:For the study, bacteria such as Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Escherichia coli ATCC25322 and Bacillus megaterium DSM32; Candida albicans FMC17 as fungi microorganisms were used.
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Conclusion & Impact:The scavenging effect of DPPH radical was observed to increase depend to increasing concentrations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Mushrooms have been used by the public for many years due to the active containts they contain as well as being consumed as a nutritious food. It is known that mushrooms have antioxidant, antihypertensive, cholesterol-lowering, liver-protective, antifibriotic, anti-inflammation, antidiabetic, antiviral and antimicrobial effects as well as immunological and anticancer properties. From the edible mushrooms Helvella leucomelaena (Pers.) Nannf. "white-footed elf cup", belong to Helvellaceae Ascomycota's is a mushroom species. The aim of this study was to determine the antimicrobial activity of H. leucomelaena by using different concentrations of extracts obtained from solvents such as methanol, ethanol and hexane, and to determine the antioxidant activity of different concentrations of extract obtained using methanol solvent. Antimicrobial activity was determined by disc diffusion method. For the study, bacteria such as Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Escherichia coli ATCC25322 and Bacillus megaterium DSM32; Candida albicans FMC17 as fungi microorganisms were used. Antioxidant activity was determined according to 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical quenching capacity method. As a result, H. leucomelaena showed the highest inhibition zone against C. albicans (16 mm) at 750 µg concentration in ethanol extract. The scavenging effect of DPPH radical was observed to increase depend to increasing concentrations.

Özet

Mantarlar içerdikleri aktif bileşenler sayesinde besleyici bir gıda olarak tüketilmelerinin yanı sıra tıbbive tedavi edici özelliklerinden dolayı uzun yıllardır halk tarafından kullanılmaktadırlar. Mantarlarınimmünolojik ve antikanser özelliklerinin yanı sıra antioksidan, antihipertensif, kolesterol düşürücü,karaciğer koruyucu, antifibriotik, antiinflamasyon, antidiyabetik, antiviral ve antimikrobiyal etkileriolduğu bilinmektedir. Yenilebilir olan mantarlardan Helvella leucomelaena (Pers.) Nannf. Ascomycota’nın Helvellaceae’ye ait bir mantar türüdür.Bu çalışmada H. leucomelaena’nın metanol, etanol ve hekzan gibi çözücülerden elde edilenekstraklarının farklı konsantrasyonları kullanılarak antimikrobiyal aktivitesinin belirlenmesi ve metanolçözücüsü kullanılarak elde edilen ekstraktının farklı konsantrasyonlarının antioksidan aktivitesininbelirlenmesi amaçlanmıştır. Antimikrobiyal aktivitesi disk difüzyon metoduna göre tespit edilmiştir.Çalışma için Staphylococcus aureus ATCC25923, Klebsiella pneumoniae ATCC700603, Escherichia coliATCC25322 ve Bacillus megaterium DSM32 gibi bakteriler, fungus olarak Candida albicans FMC17mikroorganizmaları kullanılmıştır. Antioksidan aktivitesi ise 2,2-difenil-1-pikrilhidrazil (DPPH)yöntemine göre radikal süpürücü kapasite tespit edilmiştir. Sonuçta H. leucomelaena en yüksekinhibisyon zonunu C. albicans’a karşı (16 mm) 750 µg konsantrasyonda etanol ekstresinde gösterdiğitespit edilmiştir. DPPH radikalini süpürücü etkisi ise artan konsantrasyonlara bağlı olarak arttığıgözlemlenmiştir.

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Cite This Article

KIRBAĞ, S., İNCİ, Ş., DALKILIÇ, L. K., DALKILIÇ, S. (2022). Antimicrobial And antioxidant effect of Helvella leucomelaena (Pers.) Nannf.. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, Cilt: 20 - Sayı: 2, 249–253. https://ajindex.com/articles/450636

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