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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 4

Serotyping, genetic characterization and antimicrobial susceptibility determination of Vibrio anguillarum strains isolated from farmed rainbow trout (Oncorhynchus mykiss) in the eastern Black Sea

Doğu Karadeniz’de yetiştiriciliği yapılan gökkuşağı alabalıkları (Oncorhynchus mykiss)’ndan izole edilen Vibrio anguillarum suşlarının serotiplendirilmesi, genetik karakterizasyonu ve antimikrobiyal duyarlılığının belirlenmesi

Zeynep DENGİZ BALTA
Fikri BALTA
Pages
321–328
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol

📌 3 Key Takeaways

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Objective & Hypothesis:Antibiotic resistance profile, serotyping and identification of Vibrio anguillarum isolated from rainbow trout (Oncorhynchus mykiss) cultured in cages in the Eastern Black Sea Region of Turkey, were performed in the study.
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Key Findings & Data:The rate of 97-99% similarity were found among the compared isolates.
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Conclusion & Impact:The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Antibiotic resistance profile, serotyping and identification of Vibrio anguillarum isolated from rainbow trout (Oncorhynchus mykiss) cultured in cages in the Eastern Black Sea Region of Turkey, were performed in the study. The biochemical, serological and molecular techniques were used in identification of V. anguillarum isolates. The sequenced isolates were confirmed to be similar to V. anguillarum which stored under different accession numbers in GenBank database. The rate of 97-99% similarity were found among the compared isolates. All isolates were identified to be serotype O1 by slide agglutination test results. The antibiotic susceptibility test results showed that V. anguillarum isolates were resistant 100% to sulphamethoxazole, 90.6% to ampicillin, 71.9% to erythromycin, 62.5% to oxytetracycline, 46.9% to streptomycin and 31.3% to trimethoprim-sulfamethoxazole, but susceptible to oxolinic acid, enrofloxacin and florphenicol

Özet

Bu çalışmada, Türkiye'nin Doğu Karadeniz Bölgesi'nde kafeslerde yetiştirilen gökkuşağı alabalık (Oncorhynchus mykiss)'larından izole edilen Vibrio anguillarum suşlarının identifikasyonu ve serotiplendirilmesi yapıldı ve antimikrobiyal direnç profilleri belirlendi. V. anguillarum suşlarının tanımlanmasında biyokimyasal, serolojik ve moleküler teknikler kullanıldı. Dizi analizine gönderilen şuşlar GenBank veri tabanında farklı kabul numarasıyla kayıtlı olan V. anguillarum ile karşılaştırıldı. Karşılaştırma yapılan suşlar arasında %97-99 oranında benzerlik olduğu tespit edildi. Lam aglütinasyon test sonuçlarına göre, tüm izolatların serotip O1 olduğu belirlendi. Antimikrobiyal duyarlılık test sonuçlarına göre, izolatların sulphamethoxazole (%100), ampicilline (%90,6), eritromisine (%71,9), oksitetrasiklin (%62,5), streptomisin'e (% 46,9) ve trimetoprim+sulfametoksazol (%31,3) dirençli olduğu, fakat oksolinik asit, enrofloksasin ve florfenikole duyarlı olduğu tespit edildi

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Cite This Article

BALTA, Z. D., BALTA, F. (2022). Serotyping, genetic characterization and antimicrobial susceptibility determination of Vibrio anguillarum strains isolated from farmed rainbow trout (Oncorhynchus mykiss) in the eastern Black Sea. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 4, 321–328. https://ajindex.com/articles/444664

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