SavedAdd your journal

Journals / Journal of Anatolian Environmental and Animal Sciences / 2019 / Cilt: 4 Sayı: 1

The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia

Ekzoftalmuslu Gökkuşağı Alabalıklar (Oncorhynchus mykiss)’ın Gözlerinden Lacctococcus garvieae’nın İzolasyonu

Fikri BALTA
Zeynep DENGİZ BALTA
Pages
27–33
Publication Date
2019-04-30
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The antibiotic susceptibility test results showed that L. garvieae isolates were resistant %100 to streptomycin, sulfamethoxazole and sulfamethoxazole+trimethoprim, 94.2% to ampicillin, 72.5% to oxytetracycline, 55.07% to erythromycin and 43.5% to oxolinic acid, 26.09% to enrofloxacin, % 20.3 doxycycline, 8,7% to amoxicillin and florphenicol.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, fish were sampled from 9 different fish farms in the dam lakes in the Eastern Black Sea Region between January 2016 and September 2018.
📊
Key Findings & Data:All isolates identification was carried out by using conventional biochemical tests, api 20 strep tests and PCR tests.
💡
Conclusion & Impact:The antibiotic susceptibility test results showed that L. garvieae isolates were resistant %100 to streptomycin, sulfamethoxazole and sulfamethoxazole+trimethoprim, 94.2% to ampicillin, 72.5% to oxytetracycline, 55.07% to erythromycin and 43.5% to oxolinic acid, 26.09% to enrofloxacin, % 20.3 doxycycline, 8,7% to amoxicillin and florphenicol.
🔬Design:Research Article
📍Scope:2016 Verileri
⚙️Variables:Lactococcus garvieae · api 20 strep · 16S rRNA

Abstract

In this study, fish were sampled from 9 different fish farms in the dam lakes in the Eastern Black Sea Region between January 2016 and September 2018. It was isolated twenty-two bacteria isolates from eyes of diseased rainbow trout (Oncorhynchus mykiss). All isolates identification was carried out by using conventional biochemical tests, api 20 strep tests and PCR tests. ATCC 43921 reference strain was used as a positive control. According to results of the api 20 strep test, it was determined that 20 isolates from 22 bacterial isolates were Lactococcus garvieae. The PZR test was confirmed by using the pLG-1 and pLG-2 reference genes (16S rRNA) specific for the L. garvieae species. As a result of the study, it was confirmed that there were 20 L. garvieae from 22 strains isolated from rainbow trout in the dam lakes fish farms. The antibiotic susceptibility test results showed that L. garvieae isolates were resistant %100 to streptomycin, sulfamethoxazole and sulfamethoxazole+trimethoprim, 94.2% to ampicillin, 72.5% to oxytetracycline, 55.07% to erythromycin and 43.5% to oxolinic acid, 26.09% to enrofloxacin, % 20.3 doxycycline, 8,7% to amoxicillin and florphenicol. The most effective antibiotic was found to be florfenicol, amoxicillin, doxisicline and enrofloxacin.

🔖

Cite This Article

BALTA, F., BALTA, Z. D. (2019). The Isolation of Lactococcus garvieae from Eyes of Diseased Rainbow Trout (Oncorhynchus mykiss) with Exopthalmia. Journal of Anatolian Environmental and Animal Sciences, Cilt: 4 Sayı: 1, 27–33. https://ajindex.com/articles/907748

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (8)

  1. Altun, S., Diler, O. and Adiloglu, A.K. (2004). Genotyping of Lactococcus garvieae strainsfrom rainbow trout (Oncorhynchus mykiss) by16S rDNA sequencing. Bulletin of the European Association of Fish Pathologists, 24, 119-125.
  2. Austin, B. and Austin, D.A. (1999). BacterialFish Pathogens: Disease in Farmed and Wild Fish, 3rd ed. Springer-Praxis, Chichester, UK. Austin, B. and Austin, D.A. (2007). BacterialFish Pathogens: Disease in Farmed and Wild Fish, 4th ed. Springer-Praxis, Chichester,UK. Avcı, H., Aydoğan, A., Tanrıkul, T.T. & Birincioğlu, S.S. (2010). Pathological and Microbiological Investigations in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Naturally Infected with Lactococcus garvieae. Kafkas Universitesi Veteriner Fakultesi Dergisi, 16(Suppl-B), 313-318.
  3. Balta, F. (2016). Phenotypic, serotypic and genetic characterization and antimicrobial susceptibility determination of Vibrio anguillarum, isolated from cultured sea bass (Dicentrarchus labrax L., 1758) in the southeast Black Sea, Turkey. Fresenius Environmental Bulletin, 25(10), 4393-4400.
  4. Cagırgan, H. and Tanrıkul, T.T. (1995). A new problem Enterococcus-like Infection in Rainbow Trout (Oncorhynchus mykiss) Farms in Turkey (in Turkish). Veteriner Kontrol ve Arastirma Enstitusu Dergisi, 33, 9-19.
  5. Cagırgan, H. (2004). Biotyping of Lactococcus garvieae Isolated from Turkey. Ege Journal of Fisheries and Aquatic Sciences, 21(3-4), 267-269.
  6. CLSI (2008). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria İsolated From Animals; Approved Standa-Third Edition. CLSI document M31-A3. Vol. 38, No.8, Wayne, PA. CLSI (20013). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Third Informational Supplement. CLSI document M100-S23. Vol. 33, No.1, Wayne, PA. Collins, M.D., Ash, C., Farrow, J.A.E., Wallbanks, S. and Williams, A.M, (1989). 16S ribosomal ribonucleic acid sequences analysis of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. Journal of Applied Microbiology, 73, 433-437.
  7. Didinen, B.I., Yardımcı, B., Onuk, E.E., Metin S. & Yıldırım, P. (2014). Naturally Lactococcus garvieae infection in rainbow trout (Oncorhyncus mykiss Walbaum, 1792): new histopathological observations, phenotypic and molecular identifcation. Revue de Médecine Vétérinaire, 165(1-2), 12-19.
  8. Diler, O., Altun, S., Adiloglu, A.K., Kubilay, A. and Isikli, B.I. (2002). First occurrence of streptococcosis affecting farmed rainbow trout (Onc