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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 1998 / Cilt: 27 - Sayı: 2

Simultaneous determination of morphine, codeine and 6-monoacetylmorphine in urine by GC / MS method

GC / MS metodu ile idrarda aynı anda morfin , kodein ve 6-monoasetilmorfin tayini

Yalçın DUYDU
Pages
101–113
Publication Date
2022-10-10
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This method was also applied for the urine obtained from a morphine addict.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In the present study a method was developed for simultaneous determination of morphine, codeine and 6-MAM (6-monoacetylmorphine) in urine.
📊
Key Findings & Data:We used SPE (Solid Phase Extraction) method in combination with the BSTFA + 1 % TMCS derivatization method.
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Conclusion & Impact:This method was also applied for the urine obtained from a morphine addict.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In the present study a method was developed for simultaneous determination of morphine, codeine and 6-MAM (6-monoacetylmorphine) in urine. We used SPE (Solid Phase Extraction) method in combination with the BSTFA + 1 % TMCS derivatization method. Morphine, codeine and 6-MAM were determined in urine by using GC/MS method. We determined recovery rates over 90 % for the opiates mentioned above. This method was also applied for the urine obtained from a morphine addict.

Özet

Bu çalışmada idrarda morfin, kodein ve 6-monoasetilmorfin (6-MAM)' nin aynı anda tespit edilebilmesine yönelik bir yöntem kurulmuştur. Bu yöntemde tüketme, katı faz tüketimi (Solid Phase Extraction) kolonlarında yapılmış ve N,O,bis (trimetilsilil) trifloroasetamid (BSTFA) ile %1 trimetilklorosilan (TMCS) kullanılarak türevlendirme yapılmıştır. Ölçümler kütle dedektörlü gaz kromatografisinde (GC/MS), SİM modu (seçici iyon modu) kullanılarak yapılmış ve kullandığımız opiatların her üçü içinde % 90 nın üzerinde verim (geri kazanılabilirlik) değerleri elde edilmiştir. Çalışmamızda ayrıca morfin kullanan bir şahsın idrarında da kurmuş olduğumuz bu yöntem uygulanmıştır.

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Cite This Article

DUYDU, Y. (2022). Simultaneous determination of morphine, codeine and 6-monoacetylmorphine in urine by GC / MS method. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 27 - Sayı: 2, 101–113. https://ajindex.com/articles/440849

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References (3)

  1. "Recommended methods for the detection and assay of heroin and cannabinoids in biological specimens" Manual for use by National Laboratories, ST/NAR/23, United Nations, New York, (1993). 2. Konstrand R. "Incidence of opiates, amphetamines and cocaine in hair and blood in fatal cases of heroin overdose" Forensic Sci. Int., 92(1), 29-38 (1998). 3. Wilkins D.G., Haughey H.M., Krueger G.G., Rollins D.E. "Disposition of codeine in female human hair after multiple-dose administration" J. Anal. Toxicol., 19(6), 492-498 (1995). 4. Finkle B.S. "Forensic toxicology of drug abuse: Astatus report" Anal. Chem., 44, 18-31 (1972). 5. Cone E.J., Jufer R., Darwin W.D., Needleman S.B. "Forensic drug testing for opiates. VII. Urinary excretion profile of intranasal (snorted) heroin" J. Anal. Toxicol, 20(6), 379-392 (1996). 6. Cone E.J., VVelch P., Mitchel J.M., Paul B.D. "Forensic drug testing for opiates: I. Detection of 6-acetylmorphine in urine as an indicator of recent heroin exposure
  2. Drug and assay considerations and detection times" J. Anal. Toxicol., 15, 1-7 (1991). 7. Paul B.D., Mitchel J.M., Mell L.D. "Gas chnomatography/electron impact mass fragmentometric determination of urinary 6-acetylmorphine, a metabolite of heroin" /. Anal. Toxicol., 12, 2-7 (1989). 8. Fish F., Hayes T.S. "Hydrolysis of morphine glucuronide" J. Forens. Sci., 19, 676-683 (1974). 9. Predmore D.B., Christian G.D., Loomis T.A. "Recovery of morphine from biological samples by hydrolysis and solvent extraction" J. Forens. Sci., 23, 481-489 (1974). 10. O'Neil C.L., Poklis A. "Simultaneous determination of acetylcodeine, monoacetylmorphine and other opiates in urine by GC/MS" J. Anal. Toxicol., 21 (6), 427-432 (1997). 11. Fuller D.C., Anderson W.H. "A simplifıed procedure for the determination of free codeine, free morphine and 6-acetylmorphine in urine" J. Anal. Toxicol, 16, 315-318 (1992). 12. De Giovanni N., Strano Rossi S. "Simultaneous detection of cocaine and heroine metabolites in urine by solide-phase extraction and gas chromatography-mass spectrometry" J. Chromatogr. B. Biomed. Appi, 658(1), 69-73 (1994). 13. Duydu Y. "Procedure for determination of amphetamine and methamphetamine in urine by GC/MS methode" FABAD J. Pharm. Sci., 23, 11-16, (1998). 14. Long G.L., Winefordner J.D. "Limit of detection
  3. A closer look at the IUPAC definition" Anal. Chem., 55, 712-724(1983). 15. Needleman S. B., Romberg R.W. "Limits of linearity and detection for some drugs of abuse" J. Anal. Toxicol., 14, 34