SavedAdd your journal

Journals / Fabad Eczacılık Bilimler Dergisi / 2022 / Cilt: 1 Sayı: 47

Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro

Bisfenol A ve Analoglarının MCF-7 ve HSeC Hücre Hatlarında in vitro Sitotoksik Etkisinin Değerlendirilmesi

Seda İPEK
İrem İYİGÜNDOĞDU
Aylin ÜSTÜNDAĞ
Yalçın DUYDU
Pages
13–22
Publication Date
2022-03-01
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the MCF-7 cell line, BPA and BPZ were found to have equally cytotoxic effects.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Endocrine-disrupting chemicals like bisphenol A (BPA) and its analogs have negative effects on human health.
📊
Key Findings & Data:In this study, human breast cancer cells (MCF-7) and human Sertoli cells (HSeC) were used for cell culture.
💡
Conclusion & Impact:In the MCF-7 cell line, BPA and BPZ were found to have equally cytotoxic effects.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Endokrin bozucu kimyasallar · bisfenoller · sitotoksisite

Abstract

Endocrine-disrupting chemicals like bisphenol A (BPA) and its analogs have negative effects on human health. This research aims to determine the cytotoxic effects of BPA and its four different analogs bisphenol S (BPS), bisphenol F (BPF), bisphenol Z (BPZ), bisphenol AF (BPAF) on both cancer and healthy cell lines simultaneously by performing an MTT test. In this study, human breast cancer cells (MCF-7) and human Sertoli cells (HSeC) were used for cell culture. MCF-7 and HSeC were exposed to BPA, BPS, BPF, BPZ, BPAF for 24 h. After that, the inhibitory effect of Bisphenols (IC50) was determined by measuring the absorbance. While BPF was the least cytotoxic alternative depending on the highest IC50 values in both cell lines, BPZ was found to be the most cytotoxic alternative in HSeC cell line. In the MCF-7 cell line, BPA and BPZ were found to have equally cytotoxic effects.

Özet

Bisfenol A (BPA) gibi endokrin bozucu kimyasallar ve benzerleri insan sağlığı üzerinde olumsuz etkilere sahiptir. Bu araştırma, BPA ve dört farklı analogunun bisphenol S (BPS), bisfenol F (BPF), bisfenol Z (BPZ), bisfenol AF (BPAF) hem kanser hem de sağlıklı hücre hatları üzerindeki sitotoksik etkilerini MTT testi yaparak aynı anda belirlemeyi amaçlamaktadır. Bu çalışmada, hücre kültürü için insan meme kanseri hücreleri (MCF-7) ve insan sertoli hücreleri (HSeC) kullanılmıştır. MCF-7 ve HSeC, 24 saat BPA, BPS, BPF, BPZ, BPAF’ye maruz bırakılmıştır. Daha sonra, bisfenollerin (IC50) inhibitör etkisi absorbans ölçümü ile belirlenmiştir. En yüksek IC50 değerleri nedeniyle her iki hücre hattında BPF en az sitotoksik alternatif iken, BPZ’nin HSeC hücre hattında en sitotoksik alternatif olduğu bulunmuştur. MCF-7 hücre hattında, BPA ve BPZ’nin eşit sitotoksik etkilere sahip olduğu bulunmuştur.

🔖

Cite This Article

İPEK, S., İYİGÜNDOĞDU, İ., ÜSTÜNDAĞ, A., DUYDU, Y. (2022). Evaluation of the Cytotoxic Effect of Bisphenol A and Its Analogs in MCF-7 and HSeC Cell Lines in vitro. Fabad Eczacılık Bilimler Dergisi, Cilt: 1 Sayı: 47, 13–22. https://ajindex.com/articles/919786

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (2)

  1. Aghajanpour-Mir, S. M., Zabihi, E., Akhavan-Niaki, H., Keyhani, E., Bagherizadeh, I., Biglari, S., Behjati, F. (2016). The genotoxic and cytotoxic effects of bisphenol-A (BPA) in MCF-7 cell line and amniocytes. International Journal of Molecular and Cellular Medicine, 5(1), 19. doi: 10.22088/acadpub. BUMS.5.1.19 Aris, A. (2014). Estimation of bisphenol A (BPA) concentrations in pregnant women, fetuses and nonpregnant women in Eastern Townships of Canada. Reproductive Toxicology, 45, 8-13. doi: 10.1016/j.reprotox.2013.12.006 Audebert, M., Dolo, L., Perdu, E., Cravedi, J. P., Zalko, D. (2011). Use of the γH2AX assay for assessing the genotoxicity of bisphenol A and bisphenol F in human cell lines. Archives of Toxicology, 85(11), 1463-1473. doi: 10.1007/s00204-011-0721-2
  2. Bhatnagar, A., & Anastopoulos, I. (2017). Adsorptive removal of bisphenol A (BPA) from aqueous solution: a review. Chemosphere, 168, 885-902. doi: 10.1016/j.chemosphere.2016.10.121 Chen, M., Fan, Z., Zhao, F., Gao, F., Mu, D., Zhou, Y., Shen, H., Hu, J. (2016). Occurrence and maternal transfer of chlorinated bisphenol A and nonylphenol in pregnant women and their matching embryos. Environmental Science & Technology, 50(2), 970-977. doi: 10.1021/acs.est.5b04130 Fotakis, G., & Timbrell, J. A. (2006). In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Letters, 160(2), 171-177. doi: 10.1016/j.toxlet.2005.07.001 Geens, T., Neels, H., Covaci, A. (2012). Distribution of bisphenol-A, triclosan and n-nonylphenol in human adipose tissue, liver and brain. Chemosphere, 87(7), 796-802. doi:10.1016/j.chemosphere.2012.01.002 George, V. C., & Rupasinghe, H. V. (2018). DNA damaging and apoptotic potentials of Bisphenol A and Bisphenol S in human bronchial epithelial cells. Environmental Toxicology and Pharmacology, 60, 52-57. doi: 10.1016/j.etap.2018.04.009 Gerona, R. R., Woodruff, T. J., Dickenson, C. A., Pan, J., Schwartz, J. M., Sen, S., Friesen, M. W., Fujimoto, V. Y., Hunt, P. A. (2013). Bisphenol-A (BPA), BPA glucuronide, and BPA sulfate in midgestation umbilical cord serum in a northern and central California population. Environmental Science & Technology, 47(21), 12477-12485. doi: 10.1021/es402764d Hercog, K., Štern, A., Maisanaba, S., Filipič, M., Žegura, B. (2020). Plastics in cyanobacterial blooms genotoxic effects of binary mixtures of cylindrospermopsin and bisphenols in HepG2 cells. Toxins,