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Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2021 / Cilt: 21 - Sayı: 5

The Effect of Vitamin C on Carbonic Anhydrase Isoenzymes (hCA I and II)

C Vitamininin Karbonik Anhidraz İzoenzimleri (hCA I ve II) Üzerine Etkisi

Ekrem TUNCA
Metin BÜLBÜL
Yağmur HOŞGÖR
Pages
1038–1045
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was determined that L-Ascorbic acid inhibits hCA I and hCA II isoenzymes through its non-competitive inhibition mechanism, and its Ki value was 96.8011.19 µM and 120.4622.11 µM, respectively.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Vitamins play an extremely important role in the smooth functioning of metabolic activities.
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Key Findings & Data:Vitamin C, which is one of the water-soluble vitamins, has antioxidant properties and plays a role in many biological processes such as neurotransmission and collagen synthesis.
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Conclusion & Impact:It was determined that L-Ascorbic acid inhibits hCA I and hCA II isoenzymes through its non-competitive inhibition mechanism, and its Ki value was 96.8011.19 µM and 120.4622.11 µM, respectively.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Vitamins play an extremely important role in the smooth functioning of metabolic activities. Vitamin C, which is one of the water-soluble vitamins, has antioxidant properties and plays a role in many biological processes such as neurotransmission and collagen synthesis. Carbonic anhydrase (CA) enzymes play a role in electrolyte release, pH balance, ion transport and many related processes. In our study, the inhibition effects of vitamin C, on hCA I and hCA II purified from human erythrocytes were investigated as in vitro. It was determined that L-Ascorbic acid inhibits hCA I and hCA II isoenzymes through its non-competitive inhibition mechanism, and its Ki value was 96.8011.19 µM and 120.4622.11 µM, respectively.

Özet

Metabolik faaliyetlerin sorunsuz bir şekilde yerine getirilmesinde vitaminlerin önemi son derece büyüktür. Suda çözünen bir vitamin olan C vitamini, antioksidan özellik gösterir, nörotransmitasyonda ve kolajen sentezi gibi pek çok biyolojik süreçte rol alır. Karbonik anhidraz (CA) izoenzimleri ise elektrolit salınımı, pH dengesi, iyon taşınımı ve bunlarla ilişkili birçok süreçte rol alır.Çalışmamızda, C vitamininin, insan eritrositlerinden saflaştırılan hCA I ve hCA II üzerindeki inhibisyon etkileri in vitro olarak incelenmiştir. L-Askorbik asidin hCA I ve hCA II izoenzimlerini yarışmasız inhibisyon mekanizması üzerinden inhibe ettiği, Ki değerinin sırasıyla 96.8011.19 µM ve 120.4622.11 µM olduğu belirlenmiştir.

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Cite This Article

TUNCA, E., BÜLBÜL, M., HOŞGÖR, Y. (2022). The Effect of Vitamin C on Carbonic Anhydrase Isoenzymes (hCA I and II). Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 21 - Sayı: 5, 1038–1045. https://ajindex.com/articles/429653

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