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Journals / Adnan Menderes Üniversitesi Tıp Fakültesi Dergisi / 2010 / Cilt: 11 - Sayı: 3

Kolorektal adenomlar ve adenokarsinomlarda hücre siklusunu düzenleyen proteinlerin ve apopitozla ilgili belirteçlerin ekspresyonu

Emel ÜNAL
Hedef ÖZGÜN
Muhan ERKUŞ
Nil ÇULHACI
Esra ÖZKARA
Nezih MEYDAN
İbrahim METEOĞLU
Pages
9–14
Publication Date
2022-10-10
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Bcl-2, and bcl-x expression were of no role.

📌 3 Key Takeaways

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Objective & Hypothesis:OBJECTIVE: Tumor growth is regulated by a balance between proliferation, growth arrest and cell death.
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Key Findings & Data:In this study, we have examined the expression of p27, cyclin D1, bcl-2, and bcl-x for evaluation of their roles in colon carcinoma progression.MATERIALS and METHODS: The levels of p27, cyclin D1, bcl-2, and bcl-x expression were examined by immunohistochemistry in transitional normal mucosa adjacent to adenomas (n=30), adenomas (n=30), transitional normal mucosa adjacent to adenocarcinomas (n=63), adenocarcinomas (n=63) and metastasis (n=16).
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Conclusion & Impact:Bcl-2, and bcl-x expression were of no role.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

OBJECTIVE: Tumor growth is regulated by a balance between proliferation, growth arrest and cell death. In this study, we have examined the expression of p27, cyclin D1, bcl-2, and bcl-x for evaluation of their roles in colon carcinoma progression.MATERIALS and METHODS: The levels of p27, cyclin D1, bcl-2, and bcl-x expression were examined by immunohistochemistry in transitional normal mucosa adjacent to adenomas (n=30), adenomas (n=30), transitional normal mucosa adjacent to adenocarcinomas (n=63), adenocarcinomas (n=63) and metastasis (n=16). Standard streptavidin-biotin immunperoxidase method was used for immunostaining and the stained slides were examined microscopically using semiquantitative criteria.RESULTS: Normal mucosa expressed p27 protein and adenocarcinomas displayed a decrease in the expression of this protein. Decreased expression of p27 was associated with tumor progression (p=0.026). Cyclin D1 staining was prominent in most of the adenocarcinomas and metastasis (p=0.042). Meanwhile, we could not find any relation between p27 and cyclin D1 expression. Bcl-2 and bcl-x expression also did not show any statistically significant correlation in tumor progression.CONCLUSION: The results of this study indicate that reduced p27 and cyclin D1 protein levels play an important role in progression of colon cancer. Bcl-2, and bcl-x expression were of no role.

Özet

AMAÇ: Tümör gelişimi proliferasyon, büyümede durma ve hücre ölümü arasındaki denge ile düzenlenir. Bu çalışmada, kolon karsinomunun gelişimindeki rollerinin değerlendirilmesinde p27, siklin D1, bcl-2 ve bcl-x expresyonunu inceledik.GEREÇ ve YÖNTEM: p27, siklin D1, bcl-2 ve bcl-x expresyonunun düzeyleri, adenomlara komşu normal mukozada (n=30), adenomlarda (n=30), adenokarsinomlara komşu normal mukozada (n=63), adenokarsinomlarda (n=63) ve metastazlarda (n=16) immünohistokimyasal olarak incelendi. İmmün boyama için standart streptavidin-biotin immünperoksidaz metodu kullanıldı ve boyanan slaytlar yarı kantitatif kriterler kullanarak mikroskopik olarak incelendi.BULGULAR: Normal mukozada p27 protein ekspresyonu saptandı, adenokarsinomlar ise bu protein ekspresyonunda azalma gösterdi. p27 ekspresyonunda azalma tümör progresyonu ile ilişkili idi (p=0,026). Siklin D1 boyaması adenokarsinom ve metastazların çoğunda belirgindi (p=0,042). Bu arada, p27 ve siklin D1 ekspresyonu arasında herhangi bir ilişki saptamadık. Bcl-2 ve bcl-x ekspresyonu da tümör progresyonunda istatistiksel olarak belirgin anlamlılık göstermedi.SONUÇ: Bu çalışmanın sonucu p27 ve siklin D1 protein düzeylerinin kolon kanserinin progresyonununda önemli rolü olduğunu göstermektedir. Bcl-2 ve bcl-x ekspresyonunun ise rolü saptanmamıştır.

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Cite This Article

ÜNAL, E., ÖZGÜN, H., ERKUŞ, M., ÇULHACI, N., ÖZKARA, E., MEYDAN, N., METEOĞLU, İ. (2022). Kolorektal adenomlar ve adenokarsinomlarda hücre siklusunu düzenleyen proteinlerin ve apopitozla ilgili belirteçlerin ekspresyonu. Adnan Menderes Üniversitesi Tıp Fakültesi Dergisi, Cilt: 11 - Sayı: 3, 9–14. https://ajindex.com/articles/428949

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