AI Summary & Key Takeaways
AI InsightTo erythromycin, 3% resistance was determined with only disc diffusion method.
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Abstract
In this study a total of 34 Bacillus cereus isolated from ice-cream samples in different pastry shops in Ankara, Turkey were evaluated for antibiotic resistance, which were collected from 34 samples of icecream. The isolates were identified by morphological, biochemical tests and confirmed by API 50 CHB. The susceptibility of 34 B. cereus to ampicillin (AMP), penicillin (P), tetracycline (TE), trimethoprim-sulphamethoxazole, erythromycin (E), ciprofloxacin (CIP), gentamicin (GM), and vancomycin (VA) were determined by both microdilution and disk diffusion methods. Among 34 B. cereus from isolated ice-cream samples no resistance were found to vancomycine,, gentamicin and ciprofloxacin, while they were found resistant to ampicillin (29.5%), penicillin (29.5%), and for trimethoprim-sulphamethoxazole (12%).. To tetracycline, another antibiotic investigated in this study, 6% resistance with microdilution method and 3% resistance with disc diffusion method was determined. To erythromycin, 3% resistance was determined with only disc diffusion method.
Özet
Bu çalışmada Ankara ilinde satışa sunulan çeşitli pastanelerden toplanan 34 dondurma örneğinden izole edilen toplam 34 Bacillus cereus izolatlarının antibiyotik direnci araştırıldı. İzolatlar morfolojik, biyokimyasal testlerle tanımlandı ve API 50 CHB ile doğrulandı. Ampisilin (AMP), penisilin (P), tetrasiklin (TE), trimethoprim-sulphamethoxazole, eritromisin (E), siprofloksazin (CİP), gentamisin (GM), ve vankomisin (VA) karşı sıvı mikrodilüsyon ve disk difüzyon yöntemi ile 34 B. cereus izolatının duyarlılığı belirlendi. Dondurma örneklerinden izole edilen 34 B. cereus izolatı arasında vankomisine, gentamisine, ve siprofloksazine direnç bulunmazken ampisiline (% 29.5), penisiline (% 29.5), ve trimethoprim-sufametoksazole (% 12) dirençli bulundu. Çalışmada kullanılan diğer antibiyotiklerden tetrasiklin mikrodilüsyon yöntemi ile % 6, ve disk difüzyon yöntemi ile Yo 3. Eritromisine ise sadece disk difüzyon yönteminde % 3 oranında direnç bulundu.