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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2007 / Cilt: 36 - Sayı: 4

DETERMINATION OF PROTOPINE IN FUMARIA DENSIFLORA DC. BY TLC- DENSITOMETRIC AND SPECTROPHOTOMETRIC METHOD

FUMARIA DENSIFLORA DC.’ DA OPTİK DANSİTOMETRİK VE SPEKTROFOTOMETRİK YÖNTEMLE PROTOPİN MİKTAR TAYİNİ

Tuğçe FAFAL
Mustafa Ali ÖNÜR
Pages
223–236
Publication Date
2007-12-01
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The quantitative data obtained by the use of two techniques were in good agreement

📌 3 Key Takeaways

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Objective & Hypothesis:The isoquinoline alkaloid protopine in Fumaria densiflora DC. (Fumariaceae) was quantitatively determined by TLC-densitometry and spectrophotometry.
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Key Findings & Data:In TLC-densitometry two different solvent systems as toluene : chloroform : methanol : % 25 ammonium hydroxide (5:3:1:1) and cyclohexane : diethylamine (9:1) were used and protopine was detected as 0.351 and 0.352 % respectively.
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Conclusion & Impact:The quantitative data obtained by the use of two techniques were in good agreement
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:r: Fumaria densiflora DC. · Fumariaceae · Protopin

Abstract

The isoquinoline alkaloid protopine in Fumaria densiflora DC. (Fumariaceae) was quantitatively determined by TLC-densitometry and spectrophotometry. In TLC-densitometry two different solvent systems as toluene : chloroform : methanol : % 25 ammonium hydroxide (5:3:1:1) and cyclohexane : diethylamine (9:1) were used and protopine was detected as 0.351 and 0.352 % respectively. The content of protopine in Fumaria densiflora DC. was estimated as 0.366 % in spectrophotometric method. The quantitative data obtained by the use of two techniques were in good agreement

Özet

Fumaria densiflora DC.’ daki (Fumariaceae) izokinolin alkaloitlerinden olan protopinin % miktarı optik dansitometrik ve spektrofotometrik yöntemlerle saptanmıştır. Optik dansitometrik yöntemde toluen: kloroform: metanol : %25 amonyum hidroksit (5:3:1:1) ve siklohekzan: dietilamin (9:1) olmak üzere iki farklı solvan sistemi uygulanmış olup protopin miktarı % 0.351 ve % 0.352 olarak bulunmuştur. Spektrofotometrik yöntemde ise Fumaria densiflora DC.’ nın protopin miktarı % 0.366 olarak saptanmıştır. Çalışmamız sonucunda bu iki yöntemde bulunan değerlerin birbiriyle uyumlu olduğu görülmüştür

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Cite This Article

FAFAL, T., ÖNÜR, M. A. (2007). DETERMINATION OF PROTOPINE IN FUMARIA DENSIFLORA DC. BY TLC- DENSITOMETRIC AND SPECTROPHOTOMETRIC METHOD. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 36 - Sayı: 4, 223–236. https://ajindex.com/articles/38589

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