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Journals / Tıp Araştırmaları Dergisi / 2009 / Cilt: 7 - Sayı: 2

Streptozotosin ile diyabet oluşturulan sıçanlarda timokinonun β- hücre hasarına karşı koruyucu etkileri

Protective effects of thymoquinone on β-cell damage in streptozotocin-induced diabetic rats

Mehmet KANTER
Pages
64–70
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, TQ may be clinically useful for protecting β-cells against oxidative stress.

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: The aim of the present study was to evaluate the possible protective effects of thymoquinone (TQ) against β-cell damage from streptozotocin (STZ)-induced diabetes in rats.
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Key Findings & Data:Methods: STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes.
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Conclusion & Impact:Consequently, TQ may be clinically useful for protecting β-cells against oxidative stress.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Sıçanlar · İnsülin salgılayan hücreler · Diabetes mellitus

Abstract

Purpose: The aim of the present study was to evaluate the possible protective effects of thymoquinone (TQ) against β-cell damage from streptozotocin (STZ)-induced diabetes in rats. Methods: STZ was injected intraperitoneally at a single dose of 50 mg/kg to induce diabetes. The rats in TQ treated groups were given TQ (50 mg/kg body weight) once a day orally by using intragastric intubation for 4 weeks starting 3 days prior to STZ injection. Control and diabetic untreated rats were injected with the same volume of isotonic NaCl as the diabetic treated animals that received TQ. Blood and tissue samples were obtained for biochemical and histopathological investigation. Results: The treatment of TQ caused a sharp decrease in the elevated serum glucose, and an increase in the lowered serum insulin concentrations in STZ induced diabetic rats. Islet cell degeneration and weak insulin immunohistochemical staining was observed in rats with STZ-induced diabetes. Increased intensity of staining for insulin was apparent in the TQ-treated diabetic rats. Conclusion: These findings suggest that TQ treatment exerts a therapeutic protective effect in diabetes by decreasing oxidative stress and preserving pancreatic β-cell integrity. Consequently, TQ may be clinically useful for protecting β-cells against oxidative stress.

Özet

Amaç: Bu çalışma, streptozotosin (STZ) ile diyabet oluşturulan sıçanlarda β-hücre hasarına karşı timokinonun (TQ) muhtemel koruyucu etkilerini araştırmak amacıyla planlandı. Gereç ve Yöntem: Diyabet oluşturmak için tek doz intraperitoneal STZ (50mg/kg) enjeksiyonu yapıldı. TQ uygulanan gruplardaki sıçanlara, STZ enjeksiyonundan 3 gün önce başlanarak 4 hafta boyunca her gün ağızdan 50 mg/kg TQ verildi. Kontrol ve diyabetik gruplardaki sıçanlara, TQ tedavisi alan gruptaki sıçanlarla aynı dozda izotonik NaCl uygulandı. Kan ve doku örnekleri biyokimyasal ve histopatolojik incelemeler için alındı. Bulgular: STZ ile diyabet oluşturulan sıçanlarda, TQ uygulanması yüksek serum glukoz seviyesini anlamlı bir şekilde düşürürken düşük olan serum insülin konsantrasyonunu ise arttırmıştır. Diyabetik sıçanlarda, adacık hücre dejenerasyonuyla birlikte zayıf insülin immünohistokimyasal boyanması gözlenirken, TQ uygulanmasının insülin yoğunluğunda bir artışa neden olduğu görüldü. Sonuç: Bu bulgular, TQ verilmesinin diyabetin neden olduğu oksidatif stresin azalmasında ve β- hücre bütünlüğünün korunmasında tedavi edici etkisini göstermektedir. Bu nedenle, TQ’nun oksidatif strese karşı β-hücrelerinin korunmasında klinik olarak kullanımının faydalı olabileceği kanaatindeyiz.

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Cite This Article

KANTER, M. (2009). Streptozotosin ile diyabet oluşturulan sıçanlarda timokinonun β- hücre hasarına karşı koruyucu etkileri. Tıp Araştırmaları Dergisi, Cilt: 7 - Sayı: 2, 64–70. https://ajindex.com/articles/361681

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