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Journals / Tıp Araştırmaları Dergisi / 2007 / Cilt: 5 - Sayı: 2

Effects of melatonin on serum arylesterase activity and cataract formation induced with selenite in rats

Melatoninin sıçanlarda selenitle katarakt oluşumu ve arilesteraz aktivitesi üzerine etkisi

Özcan EREL
Adil KILIÇ
Sahbettin SELEK
Pages
67–70
Publication Date
2022-10-18
DOI
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A significance was noted among the groups in cataract development (p

📌 3 Key Takeaways

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Objective & Hypothesis:Purpose: We investigated the effects of melatonin supplementation on serum Paraoxonase 1 (PON 1) arylesterase activity and on cataract formation induced with selenite in rat pups.
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Key Findings & Data:Methods: Fourty-two Wistar-albino rat pups were divided into 3 groups.
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Conclusion & Impact:A significance was noted among the groups in cataract development (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Purpose: We investigated the effects of melatonin supplementation on serum Paraoxonase 1 (PON 1) arylesterase activity and on cataract formation induced with selenite in rat pups. Methods: Fourty-two Wistar-albino rat pups were divided into 3 groups. Group 1 (n=16), received only subcutaneous (s.c.) saline on postpartum day 10. Group 2 (n=10), received s.c. sodium selenite (30 nmol/g body weight) on day 10. Group 3 (n=16), injected with s.c. sodium selenite (30 nmol / g body weight) on day 10 + intra peritoneal (i.p.) melatonin (4 ju.g / g body weight) on each day between day 7 and 14. The development of cataract was assessed weekly. The density of cataract was graded by slit-lamp biomicroscopic examination and the blood was collected on day 21. PON 1 arylesterase activity was determined in the sera. Results: All of the lenses of rat pups in Group 1 remained clean. All rat pups developed dense nuclear opacity in Group 2. Two out of 16 rat pups developed slight nuclear opacity in Group 3. A significance was noted among the groups in cataract development (p

Özet

Melatoninin sıçanlarda selenitle katarakt oluşumu ve serum arilesteraz aktivitesi üzerine etkisi Amaç: Sıçan yavrularında subkutan (s.k.) melatonin verilmesinin selenit yardımıyla oluşturulan katarakt ve serum Paraoksonaz 1 (PON 1) arilesteraz aktivitesi üzerine etkilerini irdelemek amacıyla çalışma planlandı. Gereç ve Yöntem: Kırk iki adet Wistar-albino cinsi sıçan yavrusu 3 gruba ayrıldı. Grup l'deki 16 sıçana sadece postpartum 10. günde s.k. serum fizyolojik enjeksiyonu yapıldı. Grup 2'deki 10 adet sıçana postpartum 10. günde s.k. selenit (30 nmol/g vücut ağırlığı) enjeksiyonu yapıldı. Grup 3'teki 16 adet sıçana postpartum 10. günde s.k. selenit (30 nmol/g vücut ağırlığı) enjeksiyonu, ve 7-14. günlerde her gün intra peritoneal (i.p.) melatonin (4 ug / g vücut ağırlığı) enjeksiyonu yapıldı. Her hafta katarakt gelişimi değerlendirildi. Yirmi birinci günde yarık lamba biyomikroskobisi yöntemiyle katarakt gelişimi derecelendirildi ve sıçanların kanları alınarak serum PON 1 arilesteraz aktivitesi ölçüldü. Bulgular: Grup l'deki sıçan yavrularının tümünün lenslerinin şeffaf olduğu belirlendi. Grup 2'deki tüm sıçan yavrularında yoğun nükleer opasite gelişti. Grup3'teki sıçan yavrularından 2sinde hafif nükleer opasite tespit edildi. Gruplar arasında kataraktgelişimi açısından istatistiksel olarak anlamlı fark tespit edildi (p

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Cite This Article

EREL, Ö., KILIÇ, A., SELEK, S. (2022). Effects of melatonin on serum arylesterase activity and cataract formation induced with selenite in rats. Tıp Araştırmaları Dergisi, Cilt: 5 - Sayı: 2, 67–70. https://ajindex.com/articles/598123

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