SavedAdd your journal

Journals / Records of Natural Products / 2018 / Cilt: 12 - Sayı: 5

Evaluation of Cytotoxic Activity in vitro of Charantin A Extracted from Momordica charantia

Bing LİNG
Zijun GUO
Guocai WANG
Maoxin ZHANG
Guangwen LİANG
Qiang LI
Pages
416–425
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings provide new clues regarding the role of cucurbitane-type triterpene glycosides as potential novel pesticides for pest control.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Charantins A is cucurbitane-type triterpene glycoside secondary metabolites derived from Momordica charantia.
📊
Key Findings & Data:Here, biological activities of charantin A and mode of action to Spodoptera litura ovary cell line (SpLi-221) were evaluated.
💡
Conclusion & Impact:These findings provide new clues regarding the role of cucurbitane-type triterpene glycosides as potential novel pesticides for pest control.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Özet

Charantins A is cucurbitane-type triterpene glycoside secondary metabolites derived from Momordica charantia. Here, biological activities of charantin A and mode of action to Spodoptera litura ovary cell line (SpLi-221) were evaluated. We investigated the effects of charantins A on cell growth inhibition compared with charantins B, momordicin I and azadirachtin A. Among them, charantins A showed the most potent activity against SpLi-221 cells with the lowest IC50 values, and in a time- and dose-dependent manner. Moreover, the cell morphology changed after charantin A treatment, including cytoplasm leakage, irregular morphology, and apoptotic bodies. In addition, the flow cytometry assay confirmed that charantin A promoted the occurrence of cells’ G2/M block and apoptosis, as evidenced by an increased ratio of cells in the sub-G1 phase. Furthermore, cell membrane disruption and increase of intracellular calcium levels were observed following charantin A treatment. These findings provide new clues regarding the role of cucurbitane-type triterpene glycosides as potential novel pesticides for pest control.

🔖

Cite This Article

LİNG, B., GUO, Z., WANG, G., ZHANG, M., LİANG, G., LI, Q. (2018). Evaluation of Cytotoxic Activity in vitro of Charantin A Extracted from Momordica charantia. Records of Natural Products, Cilt: 12 - Sayı: 5, 416–425. https://ajindex.com/articles/308605

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (13)

  1. Y.B. Zhang, H. Liu, C.Y. Zhu, M.X. Zhang, Y.L. Li, B. Ling and G.C. Wang (2014). Cucurbitane-type triterpenoids from the leaves of Momordica charantia, J. Asian Nat. Prod. Res. 16, 358-363
  2. N. Beloin, M. Gbeassor, K. Akpagana, J. Hudson, K. De Soussa, K. Koumaglo and J. Arnason (2005). Ethnomedicinal uses of Momordica charantia (Cucurbitaceae) in Togo and relation to its phytochemistry and biological activity, J. Ethnopharmacol. 96, 49-55
  3. J. Li, L.W. Zhang, E.Z. Yan and G.J. An (2005). The research progress on momordicoside, Food Res. Dev. 26, 21-24
  4. M. Abe and K. Matsuda (2000). Feeding deterrents from Momordica charantia leaves to cucurbitaceous feeding beetle species, Appl. Entomol. Zool. 35, 143-149
  5. X. Cao, C.Y. Zhu, M.X. Zhang and B. Ling (2015). Bioactivity of momordicin I against Ostrinia furnacalis (Lepidoptera: Pyralidae) and its effects on the metabolizing enzyme activities in larval bodies of the moth, Acta Entomol. Sin. 58, 625-633
  6. B. Ling, G.C. Wang, Y.A. Ji, M.X. Zhang and G.W. Liang (2008). Antifeedant activity and active ingredients against plutella xylostella from Momordica charantia leaves, Agric. Sci. Chin. 7, 1466-1473
  7. B. Ling, Y.L. Xiang, G.C. Wang, S.H. Chen and M.X. Zhang (2009). Antifeedant and antioviposition activities of Momordica charantia leaf ethanol extract against Liriomyza sativae, Chin. J. Appl. Ecol. 20, 836-842
  8. D.B. Mekuria, T. Kashiwagi, S. Tebayashi and C.S. Kim (2005). Cucurbitane triterpenoid oviposition deterrent from Momordica charantia to the leafminer, Liriomyza trifolii, Biosci. Biotech. Bioch. 69, 1706-1710
  9. H. Yasui, A. Kato and M. Yazawa (1998). Antifeedants to armyworms, Spodoptera litura and Pseudaletia separata, from bitter gourd leaves, Momordica charantia, J. Chem. Ecol. 24, 803-813
  10. Y. Luo, Z.J. Guo, G.C. Wang, X. Cao, M.X. Zhang and B. Ling (2013). Inhibitory effects of momordicin II on an experimental population of Ostrinia furnacalis (Güenée) and Spodoptera litura (Fabricius), Int. Conf. Biol. Med. Chem. Eng. BMCE2013, 221-227
  11. H. Liu, G.C. Wang, M.X. Zhang and B. Ling (2014). The cytotoxicology of momordicins I and II on Spodoptera litura cultured cell line SL-1, Pestic. Biochem. Physiol. 122, 110-118
  12. H. Rembold and R.S. Annadurai (2014). Azadirachtin inhibits proliferation of Sf 9 cells in monolayer culture, Z. Naturforsch. C. 48, 495-499
  13. A. Salehzadeh, A. Akhkha, W. Cushley, R. Adams, J. Kusel and R. Strang (2003). The antimitotic effe