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Journals / Hacettepe Journal of Biology and Chemistry / 2021 / Cilt: 49 - Sayı: 1

In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Esin AKBAY
Y. Doruk ARACAGÖK
Ali Hakan GÖKER
Mehmet Ali ONUR
Nilüfer CİHANGİR
Pages
25–36
Publication Date
2021-01-01
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen.
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Key Findings & Data:A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours.
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Conclusion & Impact:The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Cytotoxicity · naproxen · 6-O-desmethylnaproxen

Abstract

The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by MTT, while apoptosis was determined by AO/PI double staining method. The control group was free from any agent (DMEM only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.

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Cite This Article

AKBAY, E., ARACAGÖK, Y. D., GÖKER, A. H., ONUR, M. A., CİHANGİR, N. (2021). In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells. Hacettepe Journal of Biology and Chemistry, Cilt: 49 - Sayı: 1, 25–36. https://ajindex.com/articles/255852

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