AI Summary & Key Takeaways
AI InsightThe mean relative caspase-3 activity washigher than control group in each group at each time interval (p<0.05)and activity increased by time in N-butyl cyanoacrylate line and in2-octyl cyanoacrylate line groups (p<0.05).Conclusion: Our findings indicate that N-butyl cyanoacrylate and2-octyl cyanoacrylate cause cytotoxicity in cell-culture media.
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Abstract
Background: This study aims to investigate the cytotoxic effects andapoptotic potential of N-butyl cyanoacrylate and 2-octyl cyanoacrylateused in surgical treatment of chronic venous insufficiency.Methods: N-butyl cyanoacrylate and 2-octyl cyanoacrylate werecultured in cell-culture using human umbilical endothelial cell-line.Cytotoxicity and viability were assessed at 24 and 72 hours with lactatedehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assays, respectively. Apoptotic potential was documented at24 and 72 hours with relative caspase-3 activity.Results: The mean cytotoxicity at 24 and 72 hours were: N-butylcyanoacrylate with an area of dot/line: 37.0±3.9%/29.3±2.7% and46.4±1.6%/45.1±7.1%, 2-octyl cyanoacrylate with an area of dot/line:39.0±7.0%/37.3±4.6% and 47.0±2.3%/40.7±7.5%. Cytotoxicity increasedby time in each group (p<0.05). The mean viability at 24 and 72 hours were:N-butyl cyanoacrylate with an area of dot/line: 53.4±7.7%/72.0±5.7%and 35.7±1.9%/37.8±3.7%, 2-octyl cyanoacrylate with an area ofdot/line: 54.3±4.4%/73.5±19.9% and 33.6±2.8%/30.7±4.5%. The meanviability decreased by time in each group (p<0.05). The mean relativecaspase-3 activity at 24 and 72 hours were: control group: 0.084±0.006and 0.065±0.002, N-butyl cyanoacrylate with an area of dot/line:0.940±0.037/0.924±0.053 and 0.999±0.072/1.056±0.015, 2-octylcyanoacrylate with an area of dot/line: 0.900±0.044/0.928±0.018 and0.989±0.084/0.999±0.072. The mean relative caspase-3 activity washigher than control group in each group at each time interval (p<0.05)and activity increased by time in N-butyl cyanoacrylate line and in2-octyl cyanoacrylate line groups (p<0.05).Conclusion: Our findings indicate that N-butyl cyanoacrylate and2-octyl cyanoacrylate cause cytotoxicity in cell-culture media. We mayalso postulate that they induce apoptosis in cell-culture media.
Özet
Amaç: Bu çalışmada kronik venöz yetmezliğin cerrahi tedavisinde kullanılan N-butil siyanoakrilat ve 2-oktil siyanoakrilatın sitotoksik etkileri ve apoptotik potansiyelleri araştırıldı. Çalışma planı: N-butil siyanoakrilat ve 2-oktil siyanoakrilat insan umbilikal endotelyal hücre hattı kullanılarak hücre kültüründe kültüre edildi. Sitotoksisite ve viabilite 24. ve 72. saatlerde sırasıyla laktat dehidrogenaz ve 3-(4,5-dimetiltiyazol-2-il)-2,5-difeniltetrazolium bromid çalışmaları ile değerlendirildi. Apoptotik potansiyel kaspaz-3 aktivitesi ile 24. ve 72. saatlerde belgelendi. Bulgular: Yirmi dört ve 72. saatlerdeki ortalama sitotoksisite şöyle idi: dot/line alanı olan N-butil siyanoakrilat: %37.0±3.9/%29.3±2.7 ve %46.4±1.6/%45.1±7.1, dot/line alanı olan 2-oktil siyanoakrilat: %39.0±7.0/%37.3±4.6 ve %47.0±2.3/%40.7±7.5. Her grupta sitotoksisite zamanla arttı (p<0.05). Yirmi dört ve 72. saatlerdeki ortalama viabilite şöyle idi: dot/line alanı olan N-butil siyanoakrilat: %53.4±7.7/%72.0±5.7% ve %35.7±1.9/%37.8±3.7, dot/line alanı olan 2-oktil siyanoakrilat: %54.3±4.4/%73.5±19.9% ve %33.6±2.8/%30.7±4.5. Her grupta viabilite zamanla azaldı (p<0.05). Yirmi dört ve 72. saatlerdeki ortalama relatif kaspaz-3 aktivitesi şöyle idi: kontrol grubu: 0.084±0.006 ve 0.065±0.002, dot/line alanı olan N-butil siyanoakrilat: 0.940±0.037/0.924±0.053 ve 0.999±0.072/1.056±0.015, dot/line alanı olan 2-oktil siyanoakrilat: 0.900±0.044/0.928±0.018 ve 0.989±0.084/0.999±0.072. Her zaman aralığında, her grupta ortalama kaspaz-3 aktivitesi kontrol grubundan daha yüksek idi (p<0.05) ve aktivite N-butil siyanoakrilat ve 2-oktil siyanoakrilat gruplarında zamanla arttı (p<0.05). Sonuç: Bulgularımız, N-butil siyanoakrilat ve 2-oktil siyanoakrilatın hücre kültürü ortamında sitotoksisiteye neden olduğuna işaret etmektedir. Hücre kültürü ortamında apoptozu indüklediklerini de söyleyebilir.