In addition to spectral data, docking studies revealed that NH-A_DNA and NH-B_DNA complexes had different interaction and conformational trends to each DNA isomer.
📌 3 Key Takeaways
🎯
Objective & Hypothesis:Interaction mode of a naproxen derivative (NH) with ct-DNA was explored by absorption and fluorescence spectroscopy.
📊
Key Findings & Data:The experimental results revealed the static quenching as a result of groove binding between the naproxen derivative and ct-DNA.
💡
Conclusion & Impact:In addition to spectral data, docking studies revealed that NH-A_DNA and NH-B_DNA complexes had different interaction and conformational trends to each DNA isomer.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Quenching · Groove binding · Naproxen
Abstract
Interaction mode of a naproxen derivative (NH) with ct-DNA was explored by absorption and fluorescence spectroscopy. The experimental results revealed the static quenching as a result of groove binding between the naproxen derivative and ct-DNA. Computational studies were carried out to deeper understanding of the interactions. Molecular docking calculations shown that the interaction between NH and ct-DNA is resulted by groove binding. In addition to spectral data, docking studies revealed that NH-A_DNA and NH-B_DNA complexes had different interaction and conformational trends to each DNA isomer.
References1. M. A. Husain, Z. Yaseen, S. U. Rehman, T. Sarwar, and M. Tabish, Naproxen Intercalates with DNA and Causes Photocleavage Through ROS Generation, The FEBS Journal, 24 (2013) 6569–6580. References2. K. Gurova, New hopes from old drugs: revisiting DNA-binding small molecules as anticancer agents, Future Oncol., 5 (2009) 1685–1704. References3. D. R. Boer, A. Canals, and M. Coll, DNA-binding drugs caught in action: the latest 3D pictures of drug-DNA complexes, Dalton Trans., 3 (2009) 399-414. References4. U. Pindur, M. Jansen, and T. Lemster, Advances in DNA-ligands with groove binding, intercalating and/or alkylating activity: chemistry, DNA-binding and biology, Curr. Med. Chem., 12 ( 2005) 2805-2847. References5. L. Strekowskı and B. Wılson, Noncovalent interactions with DNA: an overview, Mutat Res-Fund Mol M., 623 ( 2007) 3–13. References6. J. Li, B. Li, Y. Wu, S. Shuang, C. Dong, and M. Choi, Luminescence and binding properties of two isoquinoline alkaloids chelerythrine and sanguinarine with ctDNA, Spectrochim Acta A Mol Biomol Spectrosc., 95 (2012) 80–85. References7. Y. Temerk, M. Ibrahim, H. Ibrahim, and M. Kotb, Interactions of an anticancer drug Formestane with single and double stranded DNA at physiological conditions, J Photochem Photobiol B., 149 (2012) 27–36. References8. L. J. Marnett and A. S. Kalgutkar, Design of selective inhibitors of cyclooxygenase-2 as nonulcerogenic anti-inflammatory agents, Curr. Opin. Chem. Biol., 2 (1998) 482–490. References9. A. S. Kalgutkar, A. B. Marnett, B. C. Crews, R. P. Remmel, and L. J. Marnett, Ester and amide derivatives of the nonsteroidal antiinflammatory drug, indomethacin, as selective cyclooxygenase-2 inhibitors, J. Med. Chem., 43 (2000) 2860–2870. References10. Y. C. Kim, Y. Karton, X. D. Ji, N. Melman, J. Linden, and K. A. Jacobson, Acyl‐hydrazide derivatives of a xanthine carboxylic congener (XCC) as selective antagonists at human A2B adenosine receptors, Drug Dev. Res., 47 (1999) 178–188. References11. M. Nakka, M.S. Begum, B.F.M. Varaprasad, L.V. Reddy, A. Bhattacharya, M. Helliwell, Naproxen and ibuprofen based acyl hydrazone derivatives: Synthesis, structure analysis and cytotoxicity studies. J Chem Pharm Res. 2 (2010) 393-409. References12. D. Sarigol, A. Uzgoren-Baran, B.C. Tel, E.I. Somuncuoglu, I. Kazkayasi, K. Ozadali-Sari, et al., Novel thiazolo [3, 2-b]-1, 2, 4-triazoles derived from naproxen with analgesic/anti-inflammatory properties: Synthesis, biological evaluation and mole