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Journals / Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi / 1998 / Cilt: 3 - Sayı: 4

Rapid diagnosis of respiratory syncytial virus in cell cultures using direct immunoperoxidase technique

Hücre kültürlerinde respiratory syncytial virüs infeksiyonunun direkt immünoperoksidaz tekniği ile hızlı tanısı

Aykut ÖZKUL
Murat ÖZSAN
Pages
258–262
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

By means of this technique, viruses and/or viral antigens were identified earliest at 36 hours postinoculation in monolayer Vero cells inoculated with RSV.

📌 3 Key Takeaways

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Objective & Hypothesis:Respiratory syncytial virus (RSV) may cause severe lower respiratory tract infections particularly in babies.
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Key Findings & Data:In this study, a direct immunoperoxidase technique in which horse radish peroxidase labelled antibody was used has been developed for rapid diagnosis of RSV.
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Conclusion & Impact:By means of this technique, viruses and/or viral antigens were identified earliest at 36 hours postinoculation in monolayer Vero cells inoculated with RSV.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Respiratory syncytial virus (RSV) may cause severe lower respiratory tract infections particularly in babies. In this study, a direct immunoperoxidase technique in which horse radish peroxidase labelled antibody was used has been developed for rapid diagnosis of RSV. By means of this technique, viruses and/or viral antigens were identified earliest at 36 hours postinoculation in monolayer Vero cells inoculated with RSV.

Özet

Bu araştırmada, özellikle bebeklerde ciddi alt solunum yolu infeksiyonu yapabilen Respiratory syncytial virüs (RSV)'ün hücre kültürlerinde hızlı tanısını sağlamak amacıyla, yaban turbu peroksidaz enzimi ile işaretli antikor kullanılan, direkt immünoperoksidaz tekniği geliştirildi. Bu teknik ile RSV inokUle edilen monolayer Vero hücrelerinde virüs ve/veya viral antijenler en erken, inokülasyonu izleyen 36. saatte identifiye edildi.

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Cite This Article

ÖZKUL, A., ÖZSAN, M. (1998). Rapid diagnosis of respiratory syncytial virus in cell cultures using direct immunoperoxidase technique. Flora İnfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi, Cilt: 3 - Sayı: 4, 258–262. https://ajindex.com/articles/224413

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References (1)

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