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Journals / JAREM / 2019 / Cilt: 9 - Sayı: 1

Alzheimer Hastalığında BACE1 ve Clusterin Genleri Ekspresyon Seviyelerinin Analizi

Analysis of the BACE1 and Clusterin Genes Expression Levels in Alzheimer’s Disease

Hani ALSAADONI
Burcu ÇAYKARA
SADRETTİN PENÇE
Saffet TÜZGEN
HALİME HANIM PENÇE
Journal
JAREM
Pages
45–49
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Sonuç: Çalışmamızda BACE1 ve Clusterin genleri kan mRNA düzeylerinin Alzheimer hastalığı ile ilişkili olduğunu belirledik.

📌 3 Key Takeaways

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Objective & Hypothesis:Amaç: Calışmamızda Alzheimer hastaları ve sağlıklı bireylerin kan örneklerinde β-amiloid öncü protein parçalayıcı enzim 1 (BACE1) ve clusterin (CLU) genlerinin mRNA ekspresyonunu araştırmayı amaçladık.
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Key Findings & Data:Yöntemler: Kan örneklerinde Clusterin ve BACE1 ekspresyon seviyeleri Real Time polimeraz zincir reaksiyonu (RT-PCR) ile incelenmiştir.
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Conclusion & Impact:Sonuç: Çalışmamızda BACE1 ve Clusterin genleri kan mRNA düzeylerinin Alzheimer hastalığı ile ilişkili olduğunu belirledik.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Amaç: Calışmamızda Alzheimer hastaları ve sağlıklı bireylerin kan örneklerinde β-amiloid öncü protein parçalayıcı enzim 1 (BACE1) ve clusterin (CLU) genlerinin mRNA ekspresyonunu araştırmayı amaçladık. Yöntemler: Kan örneklerinde Clusterin ve BACE1 ekspresyon seviyeleri Real Time polimeraz zincir reaksiyonu (RT-PCR) ile incelenmiştir. Hasta ve kontrol gruplarının ekspresyon düzeyleri 2-ΔΔCt yöntemine göre hesaplandı. İstatistiksel analiz için SPSS versiyon 22 (IBM Corp., Armonk, NY, ABD) kullanıldı ve p<0,05 istatistiksel olarak anlamlı kabul edildi. Bulgular: Alzheimer hasta grubunun kanlarında kontrol grubuna kıyasla BACE1 ve Clusterin gen ekspresyonları sırasıyla 14 ve 13 kat daha yüksek bulundu. Sonuç: Çalışmamızda BACE1 ve Clusterin genleri kan mRNA düzeylerinin Alzheimer hastalığı ile ilişkili olduğunu belirledik.

Özet

Objective: This study aimed to explore the mRNA expressions of the β-site amyloid precursor protein-cleaving enzyme 1 (BACE1) and clusterin (CLU) genes in blood samples of patients with Alzheimer’s disease (AD) and healthy subjects. Methods: The expression levels of CLU and BACE1 in blood samples of subjects were examined using the real-time polymerase chain reaction (RT-PCR). The expression levels of patient and control groups were calculated using the 2-ΔΔCt method. The Statistical Package for Social Sciences (SPSS) version 22 (IBM Corp., Armonk, NY, USA) was used for statistical analysis, and a p of ˂0.05 was accepted as statistically significant. Results: The BACE1 and CLU genes were found 14-fold and 13-fold more expressed in the blood of the AD group than on the control group, respectively. Conclusion: Our study showed that the blood mRNA levels of the BACE1 and CLU genes were associated with AD.

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Cite This Article

ALSAADONI, H., ÇAYKARA, B., PENÇE, S., TÜZGEN, S., PENÇE, H. H. (2019). Alzheimer Hastalığında BACE1 ve Clusterin Genleri Ekspresyon Seviyelerinin Analizi. JAREM, Cilt: 9 - Sayı: 1, 45–49. https://ajindex.com/articles/178276

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