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Journals / Türk Biyokimya Dergisi / 2019 / Cilt: 44 - Sayı: 4

The expression levels of miR-655-3p, miR127-5p, miR-369-3p, miR-544a in gastric cancer

Mide kanserinde miR-655-3p, miR127-5p, miR-369-3p, miR-544a ekspresyon seviyeleri

Halime Hanım PENÇE
Burcu ÇAYKARA
Süleyman BADEMLER
Hani ALSAADONI
Sadrettin PENÇE
Pages
487–491
Publication Date
2022-11-05
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

SPSS 22 wereused for statistical analysis and p 0.05) was less expressed inthe gastric cancer group than control group. miR-544a wasfound 15.5-fold more expressed in the patient group thancontrol group (fold change: 15.47, p < 0.001).Conclusion: miR127-5p, miR-544a, and miR-655-3p may beevaluated as biomarkers in gastric cancer

📌 3 Key Takeaways

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Objective & Hypothesis:Background: Gastric cancer, one of the most commoncancers in the world, is a multifactorial disease in whichenvironmental and genetic factors play a role.
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Key Findings & Data:In ourstudy, we aimed to determine the expression levels offour miRNAs (miR127-5p, miR-544a, miR-369-3p and miR655-3p) on chromosome 14q32 in gastric cancer.Materials and methods: Total RNA was isolated from bloodsamples taken from 66 gastric cancer and 66 healthy individuals.
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Conclusion & Impact:SPSS 22 wereused for statistical analysis and p 0.05) was less expressed inthe gastric cancer group than control group. miR-544a wasfound 15.5-fold more expressed in the patient group thancontrol group (fold change: 15.47, p < 0.001).Conclusion: miR127-5p, miR-544a, and miR-655-3p may beevaluated as biomarkers in gastric cancer
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background: Gastric cancer, one of the most commoncancers in the world, is a multifactorial disease in whichenvironmental and genetic factors play a role. In ourstudy, we aimed to determine the expression levels offour miRNAs (miR127-5p, miR-544a, miR-369-3p and miR655-3p) on chromosome 14q32 in gastric cancer.Materials and methods: Total RNA was isolated from bloodsamples taken from 66 gastric cancer and 66 healthy individuals. The gene expression levels determined by cDNAand quantitative real-time polymerase chain reactionwere analyzed according to the 2−∆∆Ct method. SPSS 22 wereused for statistical analysis and p 0.05) was less expressed inthe gastric cancer group than control group. miR-544a wasfound 15.5-fold more expressed in the patient group thancontrol group (fold change: 15.47, p < 0.001).Conclusion: miR127-5p, miR-544a, and miR-655-3p may beevaluated as biomarkers in gastric cancer

Özet

Amaç: Dünyadaki en yaygın kanserlerden biri olan mide kanseri, çevresel ve genetik faktörlerin rol oynadığı multifaktöriyel bir hastalıktır. Çalışmamızda mide kanserinde 14q32 kromozomunda dört miRNA’nın (miR127-5p, miR544a, miR-369-3p ve miR-655-3p) ekspresyon seviyelerini belirlemeyi amaçladık. Gereç ve Yöntemler: Total RNA 66 mide kanserinden ve 66 sağlıklı bireyden alınan kan örneklerinden izole edildi. cDNA ve kantitatif gerçek zamanlı polimeraz zincir reaksiyonu ile belirlenen gen ekspresyon seviyeleri 2−∆∆Ct metoduna göre analiz edildi. İstatistiksel analiz için SPSS22 kullanıldı ve p 0,05) mide kanseri grubunda kontrol grubundan daha az ifade edildi. miR-544a hasta grubunda kontrol grubundan 15,5 kat daha fazla ifade edildi (kat değişimi: 15,47, p < 0,001). Sonuç: miR127-5p, miR-544a ve miR-655-3p, mide kanserinde biyobelirteçler olarak değerlendirilebilir.

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Cite This Article

PENÇE, H. H., ÇAYKARA, B., BADEMLER, S., ALSAADONI, H., PENÇE, S. (2022). The expression levels of miR-655-3p, miR127-5p, miR-369-3p, miR-544a in gastric cancer. Türk Biyokimya Dergisi, Cilt: 44 - Sayı: 4, 487–491. https://ajindex.com/articles/620886

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