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Journals / İstanbul Medical Journal / 2019 / Cilt: 20 - Sayı: 5

MTHFR C677T and A1298C Gene Polymorphisms in Human Kidney Cancer Tissues

İnsan Böbrek Kanseri Dokularında MTHFR C677T ve A1298C Gen Polimorfizmleri

Alper ÖTÜNÇTEMUR
Halime Hanım PENÇE
Sadrettin PENÇE
Burcu ÇAYKARA
Hani ALSAADONI
Pages
408–412
Publication Date
2022-10-19
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Odds ratio of MTHFR C677T CC genotype was found 1.8 compared to CT genotype (p

📌 3 Key Takeaways

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Objective & Hypothesis:Introduction: The potential effect of 5,10- methylenetetrahydrofolate reductase (MTHFR) on DNA methylation, DNA repair and DNA synthesis has made MTHFR a cancer-inducing gene.
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Key Findings & Data:In this study, we aimed to evaluate C677T and A1298C gene polymorphisms in kidney cancer.
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Conclusion & Impact:Odds ratio of MTHFR C677T CC genotype was found 1.8 compared to CT genotype (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Introduction: The potential effect of 5,10- methylenetetrahydrofolate reductase (MTHFR) on DNA methylation, DNA repair and DNA synthesis has made MTHFR a cancer-inducing gene. In this study, we aimed to evaluate C677T and A1298C gene polymorphisms in kidney cancer. Methods: During the normal treatment procedure, 100 tumor and 100 surrounding healthy kidney tissue samples were obtained from patients after surgery. DNA was isolated from the tissues by DNA Isolation kit. Polymerase chain reaction andrestriction fragment length polymorphism methods were used. Genotype and allele distributions were analyzed using SPSS 22 and p0.05). MTHFRC677T CC genotype (51%) was found to be higher in kidney cancer tissues than CT genotype (34%). Odds ratio of MTHFR C677T CC genotype was found 1.8 compared to CT genotype (p

Özet

Amaç: 5,10-metilenetetrahidrofolat redüktaz’ın (MTHFR) DNA metilasyonu, DNA onarımı ve DNA sentezi üzerindeki potansiyel etkisi MTHFR’yi kanseri indükleyen bir gen yapmıştır. Çalışmamızda böbrek kanserinde C677T ve A1298C gen polimorfizmlerini değerlendirmeyi amaçladık. Yöntemler: Normal tedavi prosedürü sırasında cerrahi operasyon sonrası hastalardan 100 tümör ve 100 sağlıklı çevre böbrek dokusu örneği alındı. DNA izolasyon kiti ile dokulardan DNA izole edildi. Polimeraz zincir reaksiyonu ve restriksiyon fragman uzunluğu polimorfizmi yöntemi uygulandı. Genotip ve allel dağılımları SPSS 22 ile analiz edildi ve p0,05). Böbrek kanserli dokularda MTHFR C677T CC genotipinin (%51) CT genotipine (%34) oranla daha fazla olduğu bulundu. MTHFR C677T CC genotipinin CT genotipine oranla elde edilen tahmini rölatif riski 1,8 olarak belirlendi (p

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Cite This Article

ÖTÜNÇTEMUR, A., PENÇE, H. H., PENÇE, S., ÇAYKARA, B., ALSAADONI, H. (2022). MTHFR C677T and A1298C Gene Polymorphisms in Human Kidney Cancer Tissues. İstanbul Medical Journal, Cilt: 20 - Sayı: 5, 408–412. https://ajindex.com/articles/551903

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