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Journals / Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi / 2026 / Cilt: 5 Sayı: 1

Sıçanlarda Etanol ile İndüklenen Gastrik Ülser Modelinde Idebenon ve Krisinin Koruyucu Etkileri

THE PROTECTIVE EFFECTS OF IDEBENONE AND CHRYSIN ON ETHANOL-INDUCED GASTRIC ULCER IN RATS

Arzu Gezer(ATATURK UNIVERSITY)0000-0002-1658-2098
Ali Sefa Mendil(ATATURK UNIVERSITY)0000-0003-2722-3290
Gürsel Bedir(ATATURK UNIVERSITY)0000-0001-8859-7814
Hilal Üstündağ(Erzincan Binali Yıldırım Üniversitesi)0000-0003-3140-0755
Pages
30–37
Publication Date
2025-03-17
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Nekrotik lezyonlardaki azalma ve gastrik mukozal savunma mekanizmalarının güçlenmesi, idebenon ve krisinin gastrik ülserlerin önlenmesi ve tedavisi için terapötik potansiyel sunabileceğini düşündürmektedir.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Bu çalışmada, sıçanlarda etanol ile indüklenen gastrik ülsere karşı idebenon ve krisinin koruyucu etkileri araştırılmıştır.
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Key Findings & Data:Yirmi sekiz Sprague-Dawley erkek sıçan dört gruba ayrılmıştır: 1 ml serum fizyolojik alan kontrol grubu, gastrik ülser indüklemek için etanol (ET) (5 ml/kg) alan ET grubu ve iki tedavi grubu; biri ET'yi takiben idebenon (200 mg/kg), diğeri ET'yi takiben krisin (100 mg/kg) alan gruplar.
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Conclusion & Impact:Nekrotik lezyonlardaki azalma ve gastrik mukozal savunma mekanizmalarının güçlenmesi, idebenon ve krisinin gastrik ülserlerin önlenmesi ve tedavisi için terapötik potansiyel sunabileceğini düşündürmektedir.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Chrysin · ethyl alcohol · gastric ulcer

Abstract

Bu çalışmada, sıçanlarda etanol ile indüklenen gastrik ülsere karşı idebenon ve krisinin koruyucu etkileri araştırılmıştır. Yirmi sekiz Sprague-Dawley erkek sıçan dört gruba ayrılmıştır: 1 ml serum fizyolojik alan kontrol grubu, gastrik ülser indüklemek için etanol (ET) (5 ml/kg) alan ET grubu ve iki tedavi grubu; biri ET'yi takiben idebenon (200 mg/kg), diğeri ET'yi takiben krisin (100 mg/kg) alan gruplar. Tedaviler oral gavaj yoluyla uygulanmıştır. Gastrik mukozal hasarı ve tedavilerin koruyucu mekanizmasını değerlendirmek için, somatostatin ve aquaporin-1 düzeylerine odaklanan histopatolojik incelemeler ve immünohistokimyasal analizler gerçekleştirilmiştir. Histopatolojik incelemelerde, ET ile muamele edilen grupta şiddetli dejeneratif ve nekrotik değişiklikler gözlenmiştir. Sonuçlar, tedavi gruplarında gastrik mukozal hasarın ET grubuna kıyasla önemli ölçüde azaldığını ve nekrotik lezyonların şiddetinde anlamlı bir azalma olduğunu göstermiştir. Kantitatif analiz, tedavi gruplarında somatostatin ve aquaporin-1 ekspresyonunda anlamlı bir artış olduğunu ortaya koymuştur (P < 0,05), bu da artmış gastrik mukozal savunmayı göstermektedir. İdebenon ve krisinin, ET ile indüklenen gastrik ülserlerde histopatolojik hasarı önemli ölçüde azalttığı ve somatostatin ile aquaporin-1 ekspresyonunu artırdığı bulunmuştur. Nekrotik lezyonlardaki azalma ve gastrik mukozal savunma mekanizmalarının güçlenmesi, idebenon ve krisinin gastrik ülserlerin önlenmesi ve tedavisi için terapötik potansiyel sunabileceğini düşündürmektedir.

Özet

Objective: In this study, the protective effects of idebenone and chrysin against ethanol-induced gastric ulcers in rats were investigated. Material and methods: Twenty-eight Sprague-Dawley male rats were categorized into four groups: a control group receiving 1 ml saline, an ethanol (ET) group receiving ET (5 ml/kg) to induce gastric ulcers, and two treatment groups, one receiving ET followed by idebenone (200 mg/kg) and the other receiving ET followed by chrysin (100 mg/kg). Treatments were administered orally via gavage. Histopathological examinations and immunohistochemical analyses focusing on somatostatin and aquaporin-1 levels were performed to evaluate gastric mucosal damage and the protective mechanism of the treatments. Results: In histopathological examinations, severe degenerative and necrotic changes were observed in the ET treated group. The results showed that gastric mucosal damage was significantly reduced in the treatment groups compared to the ET group and there was a significant reduction in the severity of necrotic lesions. Quantitative analysis revealed a significant increase in somatostatin and aquaporin-1 expression in the treatment groups (P < 0.05), indicating increased gastric mucosal defence. Conclusion: Idebenone and chrysin were found to significantly mitigate histopathological damage and increase the expression of somatostatin and aquaporin-1 in ET-induced gastric ulcers. The reduction in necrotic lesions and enhancement of gastric mucosal defense mechanisms suggest that idebenone and chrysin may offer therapeutic potential for the prevention and treatment of gastric ulcers.

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Cite This Article

Gezer, A., Mendil, A. S., Bedir, G., Üstündağ, H. (2025). Sıçanlarda Etanol ile İndüklenen Gastrik Ülser Modelinde Idebenon ve Krisinin Koruyucu Etkileri. Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi, Cilt: 5 Sayı: 1, 30–37. https://doi.org/10.62425/jlasp.1561168

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References (29)

  1. Akpinar, E., Kutlu, Z., Kose, D., Aydin, P., Tavaci, T., Bayraktutan, Z., Yuksel, T. N., Yildirim, S., Eser, G., & Dincer, B. (2022). Protective effects of idebenone against sepsis induced acute lung damage. Journal of investigative surgery, 35(3), 560-568. https://doi.org/10.1080/08941939.2021.1898063
  2. Baykalir, B. G., Arslan, A. S., Mutlu, S. I., Ak, T. P., Seven, I., Seven, P. T., Yaman, M., & Gul, H. F. (2020). The protective effect of chrysin against carbon tetrachloride-induced kidney and liver tissue damage in rats. International Journal for Vitamin and Nutrition Research. https://doi.org/10.1024/0300-9831/a000653
  3. Bodis, B., Nagy, G., Németh, P., & Mózsik, G. (2001). Active water selective channels in the stomach: investigation of aquaporins after ethanol and capsaicin treatment in rats. Journal of Physiology-Paris, 95(1-6), 271-275. https://doi.org/10.1016/S0928-4257(01)00037-7
  4. Bogdanov, S. (2012). Pollen: Nutrition, functional properties, health. Magnesium, 20, 350.
  5. Cores, Á., Carmona-Zafra, N., Clerigué, J., Villacampa, M., & Menéndez, J. C. (2023). Quinones as neuroprotective agents. Antioxidants, 12(7), 1464. https://doi.org/10.3390/antiox12071464
  6. Fagundes, F. L., Piffer, G. d. M., Périco, L. L., Rodrigues, V. P., Hiruma-Lima, C. A., & Dos Santos, R. d. C. (2020). Chrysin modulates genes related to inflammation, tissue remodeling, and cell proliferation in the gastric ulcer healing. International journal of molecular sciences, 21(3), 760. https://doi.org/10.3390/ijms21030760
  7. George, M. Y., Esmat, A., Tadros, M. G., & El-Demerdash, E. (2018). In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesis. European Journal of Pharmacology, 818, 486-498. https://doi.org/10.1016/j.ejphar.2017.11.008
  8. Gezer, A., Laloglu, A., & Bölükbaş, M. K. (2023). Protective Effects of Alpha Lipoic Acid Against Ionizing Radiation-Induced Hepatotoxicity in Rats. The Eurasian Journal of Medicine, 55(2), 104. https://doi.org/10.5152/eurasianjmed.2023.0148
  9. Gezer, A., & Sari, E. K. (2023). Investigation of apoptotic and autophagic effects of chronic roflumilast use on testicular tissue in rats by immunohistochemical and immunofluorescence methods. Iranian Journal of Basic Medical Sciences, 26(3), 276. https://doi.org/10.22038/IJBMS.2023.65948.14507
  10. Hardin, J., Wallace, L., Wong, J., O’loughlin, E., Urbanski, S., Gall, D., MacNaughton, W., & Beck, P. (2004). Aquaporin expression is downregulated in a murine model of colitis and in patients with ulcerative colitis, Crohn’s disease and infectious colitis. Cell and tissue research, 318(2), 313-323. https://doi.org/10.1007/s00441-004-0932-4
  11. Inatsu, S., Ohsaki, A., & Nagata, K. (2006). Idebenone acts against growth of Helicobacter pylori by inhibiting its respiration. Antimicrobial agents and chemotherapy, 50(6), 2237-2239. https://doi.org/10.1128/aac.01118-05
  12. İpek, B. E., Yüksel, M., Cumbul, A., Ercan, F., Cabadak, H., Aydın, B., & Alican, İ. (2022). The effect of metformin on ethanol-and Indomethacin-Induced gastric ulcers in rats. The Turkish Journal of Gastroenterology, 33(9), 767. https://doi.org/10.5152/tjg.2022.21195
  13. Kaya, C. (2022). Sıçanlarda indometazin ile indüklenen mide ülseri üzerine idebenon'un etkilerinin araştırılması Tekirdağ Namık Kemal Üniversitesi.
  14. Kim, D.-U., Na, J. Y., Paik, S. S., Jee, S., Lee, Y. H., & Kim, Y. J. (2023). Mucosal distribution of somatostatin-secreting gastric Delta cells in children with gastrointestinal reflux diseases. Frontiers in Pediatrics, 11. https://doi.org/10.3389/fped.2023.1275842
  15. Kim, M., Hwang, I. Y., Lee, J.-H., Son, K. H., Jeong, C.-S., & Jung, J. (2011). Protective effect of Cimicifuga heracleifolia ethanol extract and its constituents against gastric injury. Journal of Health Science, 57(3), 289-292. https://doi.org/10.1248/jhs.57.289
  16. Küçükler, S., Kandemir, F. M., & Yıldırım, S. (2022). Protective effect of chrysin on indomethacin induced gastric ulcer in rats: role of multi-pathway regulation. Biotechnic & Histochemistry, 97(7), 490-503. https://doi.org/10.1080/10520295.2021.2014569
  17. Liao, S., Gan, L., Lv, L., & Mei, Z. (2021). The regulatory roles of aquaporins in the digestive system. Genes & Diseases, 8(3), 250-258. https://doi.org/10.1016/j.gendis.2019.12.011
  18. Mazzoni, M., Lattanzio, G., Bonaldo, A., Tagliavia, C., Parma, L., Busti, S., Gatta, P. P., Bernardi, N., & Clavenzani, P. (2021). Effect of essential oils on the oxyntopeptic cells and somatostatin and ghrelin immunoreactive cells in the european sea bass (Dicentrarchus labrax) gastric mucosa. Animals, 11(12), 3401. https://doi.org/10.3390/ani11123401
  19. Naz, S., Imran, M., Rauf, A., Orhan, I. E., Shariati, M. A., Shahbaz, M., Qaisrani, T. B., Shah, Z. A., Plygun, S., & Heydari, M. (2019). Chrysin: Pharmacological and therapeutic properties. Life sciences, 235, 116797. https://doi.org/10.1016/j.lfs.2019.116797
  20. Oncel, S., & Basson, M. D. (2022). Gut homeostasis, injury, and healing: New therapeutic targets. World Journal of Gastroenterology, 28(17), 1725. https://doi.org/10.3748/wjg.v28.i17.1725
  21. Serafim, C., Araruna, M. E., Júnior, E. A., Diniz, M., Hiruma-Lima, C., & Batista, L. (2020). A review of the role of flavonoids in peptic ulcer (2010–2020). Molecules, 25(22), 5431. https://doi.org/10.3390/molecules25225431
  22. Shastri, S., Shinde, T., Perera, A. P., Gueven, N., & Eri, R. (2020). Idebenone protects against spontaneous chronic murine colitis by alleviating endoplasmic reticulum stress and inflammatory response. Biomedicines, 8(10), 384. https://doi.org/10.3390/biomedicines8100384
  23. Shastri, S., Shinde, T., Sohal, S. S., Gueven, N., & Eri, R. (2020). Idebenone protects against acute murine colitis via antioxidant and anti-inflammatory mechanisms. International Journal of Molecular Sciences, 21(2), 484. https://doi.org/10.3390/ijms21020484
  24. Suárez-Rivero, J. M., Pastor-Maldonado, C. J., Povea-Cabello, S., Álvarez-Córdoba, M., Villalón-García, I., Munuera-Cabeza, M., Suárez-Carrillo, A., Talaverón-Rey, M., & Sánchez-Alcázar, J. A. (2021). Coenzyme Q10 analogues: Benefits and challenges for therapeutics. Antioxidants, 10(2), 236. https://doi.org/10.3390/antiox10020236
  25. Şimşek, H., Akaras, N., Gür, C., Küçükler, S., & Kandemir, F. M. (2023). Beneficial effects of Chrysin on Cadmium‐induced nephrotoxicity in rats: Modulating the levels of Nrf2/HO‐1, RAGE/NLRP3, and Caspase-3/Bax/Bcl-2 signaling pathways. Gene, 875, 147502. https://doi.org/10.1016/j.gene.2023.147502
  26. Toktay, E., & Selli, J. (2022). Histopathological Overview of Experimental Ulcer Models. https://doi.org/10.5152/eurasianjmed.2022.22312
  27. Yaghoobi, M., & Armstrong, D. (2022). Peptic ulcer disease. Yamada's Textbook of Gastroenterology, 924-976. https://doi.org/10.1002/9781119600206.ch49
  28. Ye, Y., Ran, J., Yang, B., & Mei, Z. (2023). Aquaporins in Digestive System. In Aquaporins. Springer, 145-154. https://doi.org/10.1007/978-981-19-7415-1_10
  29. Zhou, D., Yang, Q., Tian, T., Chang, Y., Li, Y., Duan, L.-R., Li, H., & Wang, S.-W. (2020). Gastroprotective effect of gallic acid against ethanol-induced gastric ulcer in rats: Involvement of the Nrf2/HO-1 signaling and anti-apoptosis role. Biomedicine & Pharmacotherapy, 126, 110075. https://doi.org/10.1016/j.biopha.2020.110075