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Journals / Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi / 2026 / Cilt: 6 Sayı: 2

Protective Effect of Active Anionic Oxygen Administration on Testicular Tissue in Rats with Experimental Sepsis

Deneysel Sepsisli Sıçanlarda Aktif Anyonik Oksijen Uygulamasının Testis Dokusu Üzerindeki Koruyucu Etkisi

Ömer Faruk Başer(KAFKAS ÜNİVERSİTESİ)0000-0002-7201-4490
Mahmut Karapehlivan(KAFKAS UNIVERSITY)0000-0003-0408-534X
Pages
162–173
Publication Date
2026-09-23
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

These findings demonstrate that AAO exerts potent antioxidant and anti-inflammatory effects, attenuates sepsis-induced testicular injury, and preserves steroidogenesis, suggesting its potential as a protective agent for sepsis-associated testicular dysfunction.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to investigate the effects of active anionic oxygen (AAO) on oxidative stress, inflammation, and steroidogenesis in sepsis-induced testicular injury in rats.
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Key Findings & Data:Testicular tissues were analyzed for oxidative stress markers (reduced glutathione [GSH], malondialdehyde [MDA], superoxide dismutase [SOD], and catalase [CAT]), inflammatory cytokines (interleukin-6 [IL-6], tumor necrosis factor-alpha [TNF-α], transforming growth factor-beta 1 [TGF-β1], and interleukin-1 beta [IL-1β]), and steroidogenic markers (3β-hydroxysteroid dehydrogenase [3β-HSD], 17β-hydroxysteroid dehydrogenase [17β-HSD], and steroidogenic acute regulatory protein [StAR]).
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Conclusion & Impact:These findings demonstrate that AAO exerts potent antioxidant and anti-inflammatory effects, attenuates sepsis-induced testicular injury, and preserves steroidogenesis, suggesting its potential as a protective agent for sepsis-associated testicular dysfunction.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Aktif Anyonik Oksijen · Enflamasyon · Oksidatif Stres

Abstract

This study aimed to investigate the effects of active anionic oxygen (AAO) on oxidative stress, inflammation, and steroidogenesis in sepsis-induced testicular injury in rats. Thirty male Wistar Albino rats were randomly assigned to four groups: Control, Sepsis (lipopolysaccharide, LPS), AAO, and Sepsis+AAO. Testicular tissues were analyzed for oxidative stress markers (reduced glutathione [GSH], malondialdehyde [MDA], superoxide dismutase [SOD], and catalase [CAT]), inflammatory cytokines (interleukin-6 [IL-6], tumor necrosis factor-alpha [TNF-α], transforming growth factor-beta 1 [TGF-β1], and interleukin-1 beta [IL-1β]), and steroidogenic markers (3β-hydroxysteroid dehydrogenase [3β-HSD], 17β-hydroxysteroid dehydrogenase [17β-HSD], and steroidogenic acute regulatory protein [StAR]). Sepsis significantly decreased GSH (p < .0001), SOD (p < .01), and CAT (p < .0001) levels, while increasing MDA (p < .0001) levels compared with the control group. In addition, IL-6 (p < .0001), TNF-α (p < .001), TGF-β1 (p < .05), and IL-1β (p < .0001) levels were significantly elevated, whereas the expression of 3β-HSD (p < .01), 17β-HSD (p < .05), and StAR (p < .01) was significantly reduced. Compared with the sepsis group, AAO treatment significantly improved antioxidant status (all p < .05), suppressed inflammatory cytokine levels (all p < .01), and enhanced steroidogenic marker expression (all p < .05). These findings demonstrate that AAO exerts potent antioxidant and anti-inflammatory effects, attenuates sepsis-induced testicular injury, and preserves steroidogenesis, suggesting its potential as a protective agent for sepsis-associated testicular dysfunction.

Özet

Bu çalışma, sıçanlarda sepsis kaynaklı testis hasarında aktif anyonik oksijen (AOO) uygulamasının oksidatif stres, enflamasyon ve steroidogenez üzerindeki etkilerini araştırmayı amaçlamıştır. Otuz iki erkek Sprague-Dawley sıçanı dört gruba ayrılmıştır: Kontrol, Sepsis (LPS), AOO ve Sepsis+AOO. Oksidatif stres parametreleri (glutatyon [GSH], malondialdehit [MDA], süperoksit dismutaz [SOD], katalaz [CAT]), enflamatuvar sitokinler (interlökin-6 [IL-6], tümör nekroz faktörü-alfa [TNF-α], transforme edici büyüme faktörü-beta1 [TGF-β1], interlökin-1 beta [IL-1β]) ve steroidojenik belirteçlerin (3β-hidroksisteroid dehidrogenaz [3β-HSD], 17β-hidroksisteroid dehidrogenaz [17β-HSD], steroidojenik akut düzenleyici protein [StAR]) biyokimyasal analizi için testis dokuları toplanmıştır. Sepsis grubunda, kontrol grubuna kıyasla GSH düzeyleri (p < .001), SOD aktivitesi (p < .01) ve CAT aktivitesi (p < .001) anlamlı derecede azalırken, MDA düzeyleri (p < .001) belirgin şekilde artmıştır. IL-6 (p < .001), TNF-α (p < .01), TGF-β1 (p < .05) ve IL-1β (p < .001) dahil olmak üzere pro-enflamatuvar sitokinler anlamlı derecede yükselirken, steroidojenik enzimlerden 3β-HSD (p < .01), 17β-HSD (p < .05) ve StAR (p < .05) sepsis grubunda anlamlı derecede azalmıştır. Sepsis+AOO grubunda ise, sepsis grubuna kıyasla AOO tedavisi GSH düzeylerini (p < .001), SOD aktivitesini (p < .05) ve CAT aktivitesini (p < .001) anlamlı derecede artırırken, MDA düzeylerini (p < .001) azaltmıştır. Ek olarak, AOO, IL-6 (p < .01), TNF-α (p < .01), TGF-β1 (p < .001) ve IL-1β düzeylerini anlamlı derecede baskılamış ve 3β-HSD (p < .001), 17β-HSD (p < .05) ve StAR (p < .01) ekspresyonunu anlamlı derecede yukarı regüle etmiştir. Bu çalışma, AOO'nun güçlü antioksidan ve anti-enflamatuvar özellikler sergilediğini, sepsis kaynaklı testis oksidatif stresini ve enflamasyonunu etkili bir şekilde baskılarken testis steroidogenezini koruduğunu ve sepsis kaynaklı testis disfonksiyonu için terapötik bir ajan olarak potansiyelini ortaya koymaktadır.

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Cite This Article

Başer, Ö. F., Karapehlivan, M. (2026). Protective Effect of Active Anionic Oxygen Administration on Testicular Tissue in Rats with Experimental Sepsis. Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi, Cilt: 6 Sayı: 2, 162–173. https://doi.org/10.62425/jlasp.1977803

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