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Journals / Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi / 2025 / Cilt: 5 Sayı: 2

Effects of Melatonin on FSH/LH/Testosterone and StAR/P450scc/17β-HSD3 Pathways in Acrylamide-Induced Testicular Toxication in Rats

Ratlarda Akrilamid İle İndüklenen Testiküler Toksikasyonda Melatoninin FSH/LH/Testosteron ve StAR/P450scc/17β-HSD3 Yolakları Üzerine Etkileri

Samet Tekin(ATATÜRK ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0003-4116-6720
Yusuf Dağ(ATATÜRK ÜNİVERSİTESİ)0000-0003-0784-5826
Furkan Aykurt(ATATÜRK ÜNİVERSİTESİ)0000-0003-3115-3969
Merve Bolat(ATATÜRK ÜNİVERSİTESİ)0000-0001-5836-5529
İsmail Bolat(ATATÜRK ÜNİVERSİTESİ)0000-0003-1398-7046
Burak Batuhan Laçin(ATATÜRK ÜNİVERSİTESİ)0000-0002-5701-3684
Burak Çınar(ATATÜRK ÜNİVERSİTESİ)0009-0005-5001-2776
Mohamad Warda(ATATÜRK ÜNİVERSİTESİ)0000-0003-0516-4023
Pages
119–125
Publication Date
2025-09-19
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Further mechanistic studies are warranted to elucidate its full protective scope.

📌 3 Key Takeaways

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Objective & Hypothesis:Acrylamide (ACR) is a toxic compound formed during high-temperature food processing that is known to induce oxidative stress and reproductive toxicity.
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Key Findings & Data:Forty adults male Wistar rats were divided into five groups: control, ACR (50 mg/kg), ACR+MEL10 (ACR + 10 mg/kg MEL), ACR+MEL20 (ACR + 20 mg/kg MEL), and MEL20 (20 mg/kg MEL alone).
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Conclusion & Impact:Further mechanistic studies are warranted to elucidate its full protective scope.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Akrilamid toksisitesi · Melatonin koruması · Oksidatif stres

Abstract

Acrylamide (ACR) is a toxic compound formed during high-temperature food processing that is known to induce oxidative stress and reproductive toxicity. This study investigated the protective effects of melatonin (MEL) on ACR-induced testicular toxicity, with a focus on hormonal regulation and steroidogenic pathways. Forty adults male Wistar rats were divided into five groups: control, ACR (50 mg/kg), ACR+MEL10 (ACR + 10 mg/kg MEL), ACR+MEL20 (ACR + 20 mg/kg MEL), and MEL20 (20 mg/kg MEL alone). Treatments were administered via intragastric gavage for 14 days. Serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured via ELISA, alongside testicular steroidogenic enzyme levels (StAR, P450scc, 17β-HSD3). Histopathological analysis was used to assess spermatogenic integrity via Johnsen scoring. ACR exposure significantly reduced the serum FSH, LH, and testosterone levels (p .05). MEL also mitigated histopathological damage, preserving seminiferous tubule integrity and spermatogenic function. These findings suggest that MEL exerts protective effects against ACR-induced testicular toxicity through its antioxidant and antiapoptotic effects, restoring steroidogenic enzyme activity and the hormonal balance. MEL supplementation may be a potential therapeutic strategy against environmental toxicants affecting male reproductive health. Further mechanistic studies are warranted to elucidate its full protective scope.

Özet

Akrilamid (ACR), oksidatif stres ve üreme toksisitesine neden olduğu bilinen yüksek sıcaklıkta gıda işleme sırasında oluşan toksik bir bileşiktir. Bu çalışmada, hormonal düzenleme ve steroidojenik yollara odaklanılarak melatoninin (MEL) ACR kaynaklı testis toksisitesi üzerindeki koruyucu etkileri araştırılmıştır. 40 yetişkin erkek Wistar sıçanı beş gruba ayrılmıştır: kontrol, ACR (50 mg/kg), ACR+MEL10 (ACR + 10 mg/kg MEL), ACR+MEL20 (ACR + 20 mg/kg MEL) ve MEL20 (sadece 20 mg/kg MEL). Tedaviler 14 gün boyunca intragastrik gavaj yoluyla uygulanmıştır. Serum folikül uyarıcı hormon (FSH), luteinize edici hormon (LH) ve testosteron seviyeleri, testis steroidojenik enzim seviyeleri (StAR, P450scc, 17β-HSD3) ile birlikte ELISA yoluyla ölçülmüştür. Histopatolojik analiz, Johnsen puanlaması yoluyla spermatogenik bütünlüğü değerlendirmek için kullanıldı. ACR maruziyeti serum FSH, LH ve testosteron seviyelerini önemli ölçüde düşürdü (p .05). MEL ayrıca histopatolojik hasarı hafifletti, seminifer tübül bütünlüğünü ve spermatogenik işlevi korudu. Bu bulgular, MEL'in antioksidan ve antiapoptotik etkileriyle ACR kaynaklı testis toksisitesine karşı koruyucu etkiler gösterdiğini, steroidojenik enzim aktivitesini ve hormonal dengeyi geri kazandırdığını göstermektedir. MEL takviyesi, erkek üreme sağlığını etkileyen çevresel toksik maddelere karşı potansiyel bir tedavi stratejisi olabilir. Tam koruyucu kapsamını açıklamak için daha fazla mekanik çalışma yapılması gerekmektedir.

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Cite This Article

Tekin, S., Dağ, Y., Aykurt, F., Bolat, M., Bolat, İ., Laçin, B. B., Çınar, B., Warda, M. (2025). Effects of Melatonin on FSH/LH/Testosterone and StAR/P450scc/17β-HSD3 Pathways in Acrylamide-Induced Testicular Toxication in Rats. Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi, Cilt: 5 Sayı: 2, 119–125. https://doi.org/10.62425/jlasp.1678282

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References (33)

  1. Adiguzel, C., Karaboduk, H., & Uzunhisarcikli, M. (2024). Protective Role of Melatonin Against Abamectin-Induced Biochemical, Immunohistochemical, and Ultrastructural Alterations in the Testicular Tissues of Rats. Microscopy and Microanalysis, 30(5), 962-977. https://doi.org/10.1093/mam/ozae080
  2. Afzal, A., Zhang, Y., Afzal, H., Saddozai, U. A. K., Zhang, L., Ji, X. Y., & Khawar, M. B. (2024). Functional role of autophagy in testicular and ovarian steroidogenesis. Frontiers in Cell and Developmental Biology, 12. https://doi.org/10.3389/fcell.2024.1384047
  3. Batuwita, B. K. H. H., Jayasinghe, J. M. J. K., Marapana, R. A. U. J., Jayasinghe, C. V. L., & Jinadasa, B. K. K. K. (2025). Reduction of Asparagine and Reducing Sugar Content, and Utilization of Alternative Food Processing Strategies in Mitigating Acrylamide Formation-A Review. Food and Bioprocess Technology, 18(3), 2101-2144. https://doi.org/10.1007/s11947-024-03565-z
  4. Bolat, I., Bolat, M., Kiliçlioglu, M., Okur, S., Gölgeli, A., Gözegir, B.,…Warda, M. (2024). Investigation of the Effects of Boric Acid against Post Operative Testicular Adhesion Caused by Experimental Laporotomy in Rats. Biological Trace Element Research. 203, 3181-3194. https://doi.org/10.1007/s12011-024-04400-4
  5. Chen, H., Ge, R. S., & Zirkin, B. R. (2009). Leydig cells: From stem cells to aging. Molecular and Cellular Endocrinology, 306(1-2), 9-16. https://doi.org/10.1016/j.mce.2009.01.023
  6. Erdem, E., Sapmaz, T., Aras, S., Sevgin, K., Baki, K. B., Topkaraoglu, S.,…Irkorucu, O. (2024). Melatonin administration in testicular damage caused by low and high-dose rate radiotherapy: An experimental study. Radiation Physics and Chemistry, 220. https://doi.org/10.1016/j.radphyschem.2024.111692
  7. Esteves, S. C., & Humaidan, P. (2025). The role of luteinizing hormone activity in spermatogenesis: from physiology to clinical practice. Reproductive Biology and Endocrinology, 23(Suppl1). https://doi.org/10.1186/s12958-024-01333-4
  8. Farag, O. M., Abd-Elsalam, R. M., El Badawy, S. A., Ogaly, H. A., Alsherbiny, M. A., & Ahmed, K. A. (2021). Portulaca oleracea seeds’ extract alleviates acrylamide-induced testicular dysfunction by promoting oxidative status and steroidogenic pathway in rats. BMC Complementary Medicine and Therapies, 21(1), 1-15. https://doi.org/10.1186/s12906-021-03286-2
  9. Farouk, S. M., Gad, F. A., Almeer, R., Abdel-Daim, M. M., & Emam, M. A. (2021). Exploring the possible neuroprotective and antioxidant potency of lycopene against acrylamide-induced neurotoxicity in rats’ brain. Biomedicine and Pharmacotherapy, 138, 111458. https://doi.org/10.1016/j.biopha.2021.111458
  10. Frungieri, M. B., Calandra, R. S., & Rossi, S. P. (2017). Local Actions of Melatonin in Somatic Cells of the Testis. International Journal of Molecular Sciences, 18(6), 1170. https://doi.org/10.3390/ijms18061170
  11. Gao, J. G., Jiang, Y., Zheng, J. T., & Nie, L. W. (2022). Pubertal exposure to acrylamide disrupts spermatogenesis by interfering with meiotic progression in male mice. Toxicology Letters, 358, 80-87. https://doi.org/10.1016/j.toxlet.2022.01.014
  12. Gul, M., Kayhan Kustepe, E., Erdemli, M. E., Altinoz, E., Gozukara Bag, H. G., Gul, S., & Gokturk, N. (2021). Protective effects of crocin on acrylamide-induced testis damage. Andrologia, 53(9), e14176. https://doi.org/10.1111/and.14176
  13. Halford, N. G., Curtis, T. Y., Muttucumaru, N., Postles, J., Elmore, J. S., & Mottram, D. S. (2012). The acrylamide problem: a plant and agronomic science issue. Journal of Experimental Botany, 63(8), 2841-2851. https://doi.org/10.1093/jxb/ers011
  14. Jimenez-Jorge, S., Guerrero, J. M., Jimenez-Caliani, A. J., Naranjo, M. C., Lardone, P. J., Carrillo-Vico, A.,…Molinero, P. (2007). Evidence for melatonin synthesis in the rat brain during development. Journal of Pineal Research, 42(3), 240-246. https://doi.org/10.1111/j.1600-079X.2006.00411.x
  15. Kumar, V., Chakraborty, A., Kural, M. R., & Roy, P. (2009). Alteration of testicular steroidogenesis and histopathology of reproductive system in male rats treated with triclosan. Reproductive Toxicology, 27(2), 177-185. https://doi.org/10.1016/j.reprotox.2008.12.002
  16. Lebda, M., Gad, S., & Gaafar, H. (2014). Effects of lipoic Acid on acrylamide induced testicular damage. Mater Sociomed, 26(3), 208-212. https://doi.org/10.5455/msm.2014.26.208-212
  17. Li, C., & Zhou, X. (2015). Melatonin and male reproduction. Clinica Chimica Acta, 446, 175-180. https://doi.org/10.1016/j.cca.2015.04.029
  18. Mogol, B. A., & Gökmen, V. (2016). Thermal process contaminants: acrylamide, chloropropanols and furan. Current Opinion in Food Science, 7, 86-92. https://doi.org/10.1016/j.cofs.2016.01.005
  19. Mokhlis, H. A., Rashed, M. H., Saleh, I. G., Eldeib, M. G., El-Husseiny, A. A., Khidr, E. G.,…Aglan, A. (2023). Hydrogen sulfide alleviates acrylamide-induced testicular toxicity in male rats. Toxicology and Environmental Health Sciences, 15(1), 41-51. https://doi.org/10.1007/s13530-022-00156-3
  20. Mukherjee, A., & Haldar, C. (2014). Photoperiodic regulation of melatonin membrane receptor (MT1R) expression and steroidogenesis in testis of adult golden hamster, Mesocricetus auratus. Journal of Photochemistry and Photobiology, 140, 374-380. https://doi.org/10.1016/j.jphotobiol.2014.08.022
  21. Murugesan, P., Muthusamy, T., Balasubramanian, K., & Arunakaran, J. (2007). Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells. Toxicology, 232(3), 170-182. https://doi.org/10.1016/j.tox.2007.01.008
  22. Owagboriaye, F. O., Dedeke, G. A., Ademolu, K. O., Olujimi, O. O., Ashidi, J. S., & Adeyinka, A. A. (2017). Reproductive toxicity of Roundup herbicide exposure in male albino rat. Experimental and Toxicologic Pathology, 69(7), 461-468. https://doi.org/10.1016/j.etp.2017.04.007
  23. Rovira, J., Diekmann, F., Ramirez-Bajo, M. J., Banon-Maneus, E., Moya-Rull, D., & Campistol, J. M. (2012). Sirolimus-associated testicular toxicity: detrimental but reversible. Transplantation, 93(9), 874-879. https://doi.org/10.1097/TP.0b013e31824bf1f0
  24. Tareke, E., Rydberg, P., Karlsson, P., Eriksson, S., & Tornqvist, M. (2002). Analysis of acrylamide, a carcinogen formed in heated foodstuffs. Journal of Agricultural and Food Chemistry, 50(17), 4998-5006. https://doi.org/10.1021/jf020302f
  25. Tijmes, M., Pedraza, R., & Valladares, L. (1996). Melatonin in the rat testis: evidence for local synthesis. Steroids, 61(2), 65-68. https://doi.org/10.1016/0039-128x(95)00197-x
  26. Tyl, R. W., Marr, M. C., Myers, C. B., Ross, W. P., & Friedman, M. A. (2000). Relationship between acrylamide reproductive and neurotoxicity in male rats. Reproductive Toxicology, 14(2), 147-157. https://doi.org/10.1016/s0890-6238(00)00066-6
  27. Walker, W. H., & Cheng, J. (2005). FSH and testosterone signaling in Sertoli cells. Reproduction, 130(1), 15-28. https://doi.org/10.1530/rep.1.00358
  28. Wang, H., Huang, P., Lie, T., Li, J., Hutz, R. J., Li, K., & Shi, F. (2010). Reproductive toxicity of acrylamide-treated male rats. Reproductive Toxicology, 29(2), 225-230. https://doi.org/10.1016/j.reprotox.2009.11.002
  29. Yan, F., Wang, L., Zhao, L., Wang, C., Lu, Q., & Liu, R. (2023). Acrylamide in food: Occurrence, metabolism, molecular toxicity mechanism and detoxification by phytochemicals. Food and Chemical Toxicology, 175, 113696. https://doi.org/10.1016/j.fct.2023.113696
  30. Yassa, H. A., George, S. M., Refaiy Ael, R., & Moneim, E. M. (2014). Camellia sinensis (green tea) extract attenuate acrylamide induced testicular damage in albino rats. Environmental Toxicology, 29(10), 1155-1161. https://doi.org/10.1002/tox.21846
  31. Yildizbayrak, N., & Erkan, M. (2018). Acrylamide disrupts the steroidogenic pathway in Leydig cells: possible mechanism of action. Toxicological and Environmental Chemistry, 100(2), 235-246. https://doi.org/10.1080/02772248.2018.1458231
  32. Yu, K., Deng, S. L., Sun, T. C., Li, Y. Y., & Liu, Y. X. (2018). Melatonin Regulates the Synthesis of Steroid Hormones on Male Reproduction: A Review. Molecules, 23(2), 447. https://doi.org/10.3390/molecules23020447
  33. Zamani, E., Shaki, F., AbedianKenari, S., & Shokrzadeh, M. (2017). Acrylamide induces immunotoxicity through reactive oxygen species production and caspase-dependent apoptosis in mice splenocytes via the mitochondria-dependent signaling pathways. Biomedicine and Pharmacotherapy, 94, 523-530. https://doi.org/10.1016/j.biopha.2017.07.033