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Dergiler / Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi / 2025 / Cilt: 5 Sayı: 2

Ratlarda Akrilamid İle İndüklenen Testiküler Toksikasyonda Melatoninin FSH/LH/Testosteron ve StAR/P450scc/17β-HSD3 Yolakları Üzerine Etkileri

Effects of Melatonin on FSH/LH/Testosterone and StAR/P450scc/17β-HSD3 Pathways in Acrylamide-Induced Testicular Toxication in Rats

Samet Tekin(ATATÜRK ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ)0000-0003-4116-6720
Yusuf Dağ(ATATÜRK ÜNİVERSİTESİ)0000-0003-0784-5826
Furkan Aykurt(ATATÜRK ÜNİVERSİTESİ)0000-0003-3115-3969
Merve Bolat(ATATÜRK ÜNİVERSİTESİ)0000-0001-5836-5529
İsmail Bolat(ATATÜRK ÜNİVERSİTESİ)0000-0003-1398-7046
Burak Batuhan Laçin(ATATÜRK ÜNİVERSİTESİ)0000-0002-5701-3684
Burak Çınar(ATATÜRK ÜNİVERSİTESİ)0009-0005-5001-2776
Mohamad Warda(ATATÜRK ÜNİVERSİTESİ)0000-0003-0516-4023
Sayfa
119–125
Yayın Tarihi
2025-09-19
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

Bu bulgular, MEL'in antioksidan ve antiapoptotik etkileriyle ACR kaynaklı testis toksisitesine karşı koruyucu etkiler gösterdiğini, steroidojenik enzim aktivitesini ve hormonal dengeyi geri kazandırdığını göstermektedir.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Akrilamid (ACR), oksidatif stres ve üreme toksisitesine neden olduğu bilinen yüksek sıcaklıkta gıda işleme sırasında oluşan toksik bir bileşiktir.
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Temel Bulgular & Veri:Bu çalışmada, hormonal düzenleme ve steroidojenik yollara odaklanılarak melatoninin (MEL) ACR kaynaklı testis toksisitesi üzerindeki koruyucu etkileri araştırılmıştır. 40 yetişkin erkek Wistar sıçanı beş gruba ayrılmıştır: kontrol, ACR (50 mg/kg), ACR+MEL10 (ACR + 10 mg/kg MEL), ACR+MEL20 (ACR + 20 mg/kg MEL) ve MEL20 (sadece 20 mg/kg MEL).
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Sonuç & Katkı:Bu bulgular, MEL'in antioksidan ve antiapoptotik etkileriyle ACR kaynaklı testis toksisitesine karşı koruyucu etkiler gösterdiğini, steroidojenik enzim aktivitesini ve hormonal dengeyi geri kazandırdığını göstermektedir.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Akrilamid toksisitesi · Melatonin koruması · Oksidatif stres

Özet

Akrilamid (ACR), oksidatif stres ve üreme toksisitesine neden olduğu bilinen yüksek sıcaklıkta gıda işleme sırasında oluşan toksik bir bileşiktir. Bu çalışmada, hormonal düzenleme ve steroidojenik yollara odaklanılarak melatoninin (MEL) ACR kaynaklı testis toksisitesi üzerindeki koruyucu etkileri araştırılmıştır. 40 yetişkin erkek Wistar sıçanı beş gruba ayrılmıştır: kontrol, ACR (50 mg/kg), ACR+MEL10 (ACR + 10 mg/kg MEL), ACR+MEL20 (ACR + 20 mg/kg MEL) ve MEL20 (sadece 20 mg/kg MEL). Tedaviler 14 gün boyunca intragastrik gavaj yoluyla uygulanmıştır. Serum folikül uyarıcı hormon (FSH), luteinize edici hormon (LH) ve testosteron seviyeleri, testis steroidojenik enzim seviyeleri (StAR, P450scc, 17β-HSD3) ile birlikte ELISA yoluyla ölçülmüştür. Histopatolojik analiz, Johnsen puanlaması yoluyla spermatogenik bütünlüğü değerlendirmek için kullanıldı. ACR maruziyeti serum FSH, LH ve testosteron seviyelerini önemli ölçüde düşürdü (p .05). MEL ayrıca histopatolojik hasarı hafifletti, seminifer tübül bütünlüğünü ve spermatogenik işlevi korudu. Bu bulgular, MEL'in antioksidan ve antiapoptotik etkileriyle ACR kaynaklı testis toksisitesine karşı koruyucu etkiler gösterdiğini, steroidojenik enzim aktivitesini ve hormonal dengeyi geri kazandırdığını göstermektedir. MEL takviyesi, erkek üreme sağlığını etkileyen çevresel toksik maddelere karşı potansiyel bir tedavi stratejisi olabilir. Tam koruyucu kapsamını açıklamak için daha fazla mekanik çalışma yapılması gerekmektedir.

Abstract

Acrylamide (ACR) is a toxic compound formed during high-temperature food processing that is known to induce oxidative stress and reproductive toxicity. This study investigated the protective effects of melatonin (MEL) on ACR-induced testicular toxicity, with a focus on hormonal regulation and steroidogenic pathways. Forty adults male Wistar rats were divided into five groups: control, ACR (50 mg/kg), ACR+MEL10 (ACR + 10 mg/kg MEL), ACR+MEL20 (ACR + 20 mg/kg MEL), and MEL20 (20 mg/kg MEL alone). Treatments were administered via intragastric gavage for 14 days. Serum follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured via ELISA, alongside testicular steroidogenic enzyme levels (StAR, P450scc, 17β-HSD3). Histopathological analysis was used to assess spermatogenic integrity via Johnsen scoring. ACR exposure significantly reduced the serum FSH, LH, and testosterone levels (p .05). MEL also mitigated histopathological damage, preserving seminiferous tubule integrity and spermatogenic function. These findings suggest that MEL exerts protective effects against ACR-induced testicular toxicity through its antioxidant and antiapoptotic effects, restoring steroidogenic enzyme activity and the hormonal balance. MEL supplementation may be a potential therapeutic strategy against environmental toxicants affecting male reproductive health. Further mechanistic studies are warranted to elucidate its full protective scope.

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Bu Makaleye Atıf Yap

Tekin, S., Dağ, Y., Aykurt, F., Bolat, M., Bolat, İ., Laçin, B. B., Çınar, B., Warda, M. (2025). Ratlarda Akrilamid İle İndüklenen Testiküler Toksikasyonda Melatoninin FSH/LH/Testosteron ve StAR/P450scc/17β-HSD3 Yolakları Üzerine Etkileri. Laboratuvar Hayvanları Bilimi ve Uygulamaları Dergisi, Cilt: 5 Sayı: 2, 119–125. https://doi.org/10.62425/jlasp.1678282

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