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Journals / Bozok Veterinary Sciences / 2024 / Cilt: 5 Sayı: 2

Procalcitonin and Cytokin Levels in Calves with Diarhoe

İshalli Buzağılarda Prokalsitonin ve Sitokin Düzeyleri

Oğuzhan Aslandoğan0009-0002-1550-727X
Vehbi Güneş(Erciyes Üniversitesi Veteriner Fakültesi)0000-0002-4047-4409
Pages
35–42
Publication Date
2024-12-31
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, it was evaluated that serum PCT levels are a reliable inflammatory biomarker in infectious calf diarrhea and can be used in monitoring and determining the severity of the disease.

📌 3 Key Takeaways

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Objective & Hypothesis:Procalcitonin (PCT) is an acute phase protein released in thyroid C cells.
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Key Findings & Data:It is considered a measurable marker in provocative reactions in bacterial and parasitic infections due to its ability to increase rapidly after the production of specific cytokines (TNF-α, IL-6 and IL-8).
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Conclusion & Impact:As a result, it was evaluated that serum PCT levels are a reliable inflammatory biomarker in infectious calf diarrhea and can be used in monitoring and determining the severity of the disease.
🔬Design:Klinik Araştırma
📍Scope:Comprehensive Sample
⚙️Variables:Diyare · prokalsitonin · buzağı

Abstract

Procalcitonin (PCT) is an acute phase protein released in thyroid C cells. It is considered a measurable marker in provocative reactions in bacterial and parasitic infections due to its ability to increase rapidly after the production of specific cytokines (TNF-α, IL-6 and IL-8). This study aimed to define the relationships between procalcitonin (PCT) and selected proinflammatory cytokine (TNF-α, IL-6) levels in the serum of calves with diarrhea and the severity of the disease diagnosis. For this purpose, 45 diarrheal calves of different breeds (Holstein, Simmental, Jersey) aged 1-30 days and 15 healthy calves (Group 1) aged 1-30 days were included in the study. Diarrheal calves used in the study were divided into 3 groups. Group 2: mild (n=15), Group 3: severe (n=15), and Group 4: comatose (n=15) patient groups. Blood samples suitable for complete blood count, blood gas analysis and ELISA analysis were taken from the jugular vein of the calves. TNF-α, IL-6 and PCT analyzes were performed in accordance with the application procedure of commercial ELISA kits. The mean PCT concentrations of calves with mild (456.54 ± 115.35 ng/ml), severe (473.19 ± 120.50 ng/ml) and comatose (459.51 ± 99.14 ng/ml) diarrhea were statistically higher than the values obtained from healthy calves (315.70 ± 83.90 ng/ml). was found to be significantly higher (p≤0.01). As a result, it was evaluated that serum PCT levels are a reliable inflammatory biomarker in infectious calf diarrhea and can be used in monitoring and determining the severity of the disease.

Özet

Prokalsitonin (PCT), tiroid C hücrelerinde salınan bir akut faz proteinidir. Özel sitokinlerin (TNF-α, IL-6 ve IL-8) üretiminden sonra hızlı bir şekilde artma kabiliyeti nedeniyle bakteriyel ve parazitik enfeksiyonlardaki provokatif reaksiyonlarda ölçülebilir bir belirteç olarak kabul edilmektedir. Bu çalışmada, ishalli buzağıların serumunda prokalsitonin (PCT) ve seçilmiş proinflamatuar sitokin (TNF-α, IL-6) düzeyleri ile hastalığın teşhisi şiddeti arasındaki ilişkilerin tanımlanması amaçlandı. Bu amaçla 1-30 günlük yaşta farklı ırklardan (holstein, simental, jersey) 45 adet ishalli buzağı ve 15 adet 1-30 günlük sağlıklı buzağı (Grup 1) çalışmaya dahil edildi. Çalışmada kullanılan ishalli buzağılar 3 gruba ayrıldı. Grup 2: hafif (n=15), Grup 3: şiddetli (n=15), Grup 4: ise komatöz (n=15) hasta gruplarını oluşturdu. Buzağıların vena jugularis’lerinden tam kan sayımı, kan gazı analizleri ve ELISA analizleri için uygun kan örnekleri alındı. TNF-α, IL-6 ve PCT analizleri ticari ELISA kitlerinin uygulama prosedürüne uygun olarak yapıldı. Hafif (456.54 ± 115.35 ng/ml), şiddetli (473.19 ± 120.50 ng/ml) ve komatöz (459.51 ± 99.14 ng/ml) ishalli buzağıların ortalama PCT konsantrasyonları, sağlıklı buzağılardan (315.70 ± 83.90 ng/ml) elde edilen değerlerden istatistiksel olarak anlamlı düzeyde yüksek bulundu (p≤0.01). Sonuç olarak serum PCT düzeylerinin enfeksiyöz buzağı ishallerinde güvenilir bir yangısel biyobelirteç olduğu, hastalığın takibi ve şiddetinin belirlenmesinde kullanılabileceği değerlendirilmiştir.

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Cite This Article

Aslandoğan, O., Güneş, V. (2024). Procalcitonin and Cytokin Levels in Calves with Diarhoe. Bozok Veterinary Sciences, Cilt: 5 Sayı: 2, 35–42. https://doi.org/10.58833/bozokvetsci.1500824

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