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Journals / Bozok Veterinary Sciences / 2026 / Sayı: 2026

EARLY HEPATOPROTECTIVE EFFECTS OF GINKGO BILOBA EXTRACT AGAINST DIETHYLNITROSAMINE-INDUCED DAMAGE IN RABBITS

Tavşanlarda Dietilnitrozamin ile İndüklenen Hasara Karşı Ginkgo biloba Ekstresinin Erken Dönem Hepatoprotektif Etkileri

Abdulsamed Kükürt(KAFKAS ÜNİVERSİTESİ)0000-0002-3603-0506
Emine Atakişi(KAFKAS ÜNİVERSİTESİ)0000-0002-5685-1870
Oğuz Merhan(KAFKAS UNIVERSITY)0000-0002-3399-0667
Recai Aci(ADNAN MENDERES UNIVERSITY)0000-0002-1517-3356
Onur Atakişi(KAFKAS UNIVERSITY)0000-0003-1183-6076
Hasan Özen(BALIKESİR ÜNİVERSİTESİ)0000-0002-6820-2536
Ayla Özcan(GAZI UNIVERSITY)0000-0001-6442-8047
Mustafa Ermiş(ERCIYES UNIVERSITY)0000-0003-2267-1238
Pages
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Publication Date
2026-10-28
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

GB was associated with attenuation of early DEN-related oxidative and inflammatory responses, but prevention of tumor formation was not demonstrated.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study evaluated whether Ginkgo biloba extract (GB) attenuates early oxidative, inflammatory, and apoptotic changes induced by diethylnitrosamine (DEN) in rabbits.
📊
Key Findings & Data:Thirty-two rabbits were allocated to control, DEN, DEN+GB, and GB groups (n = 8 each).
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Conclusion & Impact:GB was associated with attenuation of early DEN-related oxidative and inflammatory responses, but prevention of tumor formation was not demonstrated.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Apoptozis · Dietilnitrozamin · Ginkgo biloba

Abstract

This study evaluated whether Ginkgo biloba extract (GB) attenuates early oxidative, inflammatory, and apoptotic changes induced by diethylnitrosamine (DEN) in rabbits. Thirty-two rabbits were allocated to control, DEN, DEN+GB, and GB groups (n = 8 each). DEN was administered once intraperitoneally at 100 mg/kg, and GB was given as one oral capsule daily for 60 days. Plasma tumor necrosis factor alpha (TNF-α) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) were measured on days 7, 30, and 60; hepatic DNA fragmentation was assessed by TUNEL. On day 7, TNF-α and 8-OHdG were higher in the DEN group than in the control and GB groups (p < 0.05), while DEN+GB showed intermediate values. On day 30, TNF-α remained higher in the DEN group than in all other groups (p < 0.05). Neither marker differed among groups on day 60. Few TUNEL-positive cells occurred in the DEN group and were absent or sparse elsewhere. GB was associated with attenuation of early DEN-related oxidative and inflammatory responses, but prevention of tumor formation was not demonstrated.

Özet

Bu çalışmada, tavşanlarda dietilnitrozaminin (DEN) oluşturduğu erken oksidatif, inflamatuvar ve apoptotik değişikliklere karşı Ginkgo biloba ekstresi (GB) değerlendirildi. Otuz iki tavşan kontrol, DEN, DEN+GB ve GB gruplarına ayrıldı (her grupta n = 8). DEN tek doz 100 mg/kg intraperitoneal, GB ise 60 gün boyunca günde bir oral kapsül olarak uygulandı. Plazma tümör nekroz faktörü alfa (TNF-α) ve 8-hidroksi-2′-deoksiguanozin (8-OHdG) düzeyleri 7., 30. ve 60. günlerde; karaciğer DNA parçalanması TUNEL yöntemiyle değerlendirildi. Yedinci günde TNF-α ve 8-OHdG, DEN grubunda kontrol ve GB gruplarından yüksekti (p < 0,05); DEN+GB grubu ara değerler gösterdi. Otuzuncu günde TNF-α DEN grubunda diğer gruplardan yüksek kalırken (p < 0,05), 60. günde iki belirteç için de fark bulunmadı. DEN grubunda az sayıda TUNEL-pozitif hücre gözlendi; diğer gruplarda bu hücreler yoktu veya seyrekti. GB erken oksidatif ve inflamatuvar yanıtın sınırlanmasıyla ilişkiliydi; ancak tümör oluşumunun önlendiği gösterilmedi.

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Cite This Article

Kükürt, A., Atakişi, E., Merhan, O., Aci, R., Atakişi, O., Özen, H., Özcan, A., Ermiş, M. (2026). EARLY HEPATOPROTECTIVE EFFECTS OF GINKGO BILOBA EXTRACT AGAINST DIETHYLNITROSAMINE-INDUCED DAMAGE IN RABBITS. Bozok Veterinary Sciences, Sayı: 2026. https://doi.org/10.58833/bozokvetsci.2046499

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