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Journals / Journal of Agriculture / 2026 / Cilt: 9 Sayı: 2

Determination of the Phenolic Composition Profile of Walnut Leaf (Juglans regia L.) Extract Obtained Using an Ethanol-Water Solvent System by the HPLC-DAD Method and Its Conversion into a Cosmetic Skin Care Cream

Etanol-Su Çözücü Sistemi ile Elde Edilen Ceviz Yaprağı (Juglans regia L.) Ekstraktının HPLC-DAD Yöntemiyle Fenolik Bileşim Profilinin Belirlenmesi ve Kozmetik Bakım Kremine Dönüştürülmesi

Musa Karadağ(ığdır üniversitesi)0000-0003-2498-3403
Pages
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Publication Date
2026-10-02
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The findings indicate that the walnut leaf extract contains substantial levels of phenolic acids and flavonoids—particularly gallic acid, catechin, rutin, and quercetin—consistent with the general trends reported in the existing literature.

📌 3 Key Takeaways

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Objective & Hypothesis:Walnut (Juglans regia L.) leaves are a plant material widely valorized in traditional medicine, cosmetic formulations, and the food supplement industry due to their rich phenolic composition.
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Key Findings & Data:In this study, the phenolic composition profile of an extract (10000 ppm) obtained from walnut leaves using an ethanol–water solvent system was investigated by reversed-phase high-performance liquid chromatography with diode-array detection (HPLC-DAD).
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Conclusion & Impact:The findings indicate that the walnut leaf extract contains substantial levels of phenolic acids and flavonoids—particularly gallic acid, catechin, rutin, and quercetin—consistent with the general trends reported in the existing literature.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Juglans regia · ceviz yaprağı · HPLC-DAD

Abstract

Walnut (Juglans regia L.) leaves are a plant material widely valorized in traditional medicine, cosmetic formulations, and the food supplement industry due to their rich phenolic composition. In this study, the phenolic composition profile of an extract (10000 ppm) obtained from walnut leaves using an ethanol–water solvent system was investigated by reversed-phase high-performance liquid chromatography with diode-array detection (HPLC-DAD). The analysis was performed at 300 nm signal and 500 nm reference wavelengths over a 0–65 min run time. A total of 16 peaks with retention times (tR) ranging from 3.266 to 35.712 min were identified in the resulting chromatogram: ascorbic acid, gallic acid, protocatechuic acid, catechin, hydroxybenzoic acid, gentisic acid, p-coumaric acid, rutin, ferulic acid, naringin, o-coumaric acid, neohesperidin, coumarin, resveratrol, quercetin, and trans-cinnamic acid. The peaks with the highest absorbance intensity were ascorbic acid (tR=3.266 min) and catechin (tR=6.440 min), both eluting in the early retention window (tR<10 min), which accounted for a considerable proportion of the total peak area. The findings indicate that the walnut leaf extract contains substantial levels of phenolic acids and flavonoids—particularly gallic acid, catechin, rutin, and quercetin—consistent with the general trends reported in the existing literature. These results support the potential use of walnut leaf extract as a natural phenolic source with antioxidant potential in cosmetic, food supplement, and functional product formulations.

Özet

Ceviz (Juglans regia L.) yaprakları; geleneksel tıpta, kozmetik formülasyonlarda ve gıda takviyesi endüstrisinde yaygın biçimde değerlendirilen, zengin fenolik bileşim içeriğine sahip bir bitkisel materyaldir. Bu çalışmada, ceviz yapraklarından etanol-su çözücü sistemi kullanılarak elde edilen ekstraktın (10000 ppm) fenolik bileşim profili ters faz yüksek performanslı sıvı kromatografisi-diyot dizi dedektörü (HPLC-DAD) yöntemiyle incelenmiştir. Analiz 300 nm sinyal ve 500 nm referans dalga boylarında, 0-65 dakikalık analiz süresinde gerçekleştirilmiştir. Elde edilen kromatogramda alıkonma zamanı (tR) 3,266-35,712 dakika aralığında değişen toplam 16 pik tanımlanmıştır: askorbik asit, gallik asit, protokatekuik asit, kateşin, hidroksibenzoik asit, gentisik asit, parakumarik asit, rutin ferulik asit naringin o-kumarik asit neohesperidin kumarin resveratrol kuersetin ve trans-sinamik asit. Kromatogramda en yüksek absorbans şiddetine sahip pikler askorbik asit (tR=3.266 dk) ve kateşin (tR=6.440 dk) olarak belirlenmiş; erken eluasyon bölgesinde (tR<10 dk) yoğunlaşan bu bileşiklerin toplam pik alanının önemli bir kısmını oluşturduğu gözlenmiştir. Bulgular ceviz yaprağı ekstraktının başta gallik asit kateşin rutin ve kuersetin olmak üzere önemli düzeyde fenolik asit ve flavonoid içeriğine sahip olduğunu göstermekte olup literatürdeki önceki çalışmalarla genel eğilim bakımından örtüşmektedir. Sonuçlar ceviz yaprağı ekstraktının antioksidan potansiyel taşıyan doğal bir fenolik kaynak olarak kozmetik gıda takviyesi ve fonksiyonel ürün formülasyonlarında değerlendirilebileceğini desteklemektedir.

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Cite This Article

Karadağ, M. (2026). Determination of the Phenolic Composition Profile of Walnut Leaf (Juglans regia L.) Extract Obtained Using an Ethanol-Water Solvent System by the HPLC-DAD Method and Its Conversion into a Cosmetic Skin Care Cream. Journal of Agriculture, Cilt: 9 Sayı: 2. https://doi.org/10.46876/ja.2022414

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