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Journals / Journal of Agriculture / 2026 / Cilt: 9 Sayı: 1

Linking Total Cell Protein Profiles Revealed by SDS-PAGE in Erwinia amylovora Strains from Pear Orchards in Türkiye to Virulence Level Variation

Türkiye’deki armut bahçelerinden elde edilen Erwinia amylovora strainlerinin SDS-PAGE ile ortaya konulan toplam hücre protein profillerinin virülenslik düzey değişimleriyle ilişkilendirilmesi

Ayşegül Gedük(SELÇUK ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ)0000-0003-0299-1701
Kubilay Kurtuluş Baştaş(SELCUK UNIVERSITY)0000-0002-2367-1849
Pages
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Publication Date
2026-07-08
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The SDS-PAGE-derived profiles revealed a predominantly conserved proteomic architecture, with overall similarity values ranging from 70% to 100%.

📌 3 Key Takeaways

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Objective & Hypothesis:Erwinia amylovora, the causal agent of fire blight, is a globally significant and highly destructive pathogen of pome fruit species, imposing severe economic constraints on sustainable agricultural systems.
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Key Findings & Data:In this study, the total cellular protein profiles of 78 E. amylovora strains collected from pear orchards across seven distinct regions of Türkiye were analyzed to elucidate phenotypic relationships and assess intra-population diversity.
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Conclusion & Impact:The SDS-PAGE-derived profiles revealed a predominantly conserved proteomic architecture, with overall similarity values ranging from 70% to 100%.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:ateş yanıklığı · Pyrus communis · fenotipik çeşitlilik

Abstract

Erwinia amylovora, the causal agent of fire blight, is a globally significant and highly destructive pathogen of pome fruit species, imposing severe economic constraints on sustainable agricultural systems. In this study, the total cellular protein profiles of 78 E. amylovora strains collected from pear orchards across seven distinct regions of Türkiye were analyzed to elucidate phenotypic relationships and assess intra-population diversity. Protein extraction was conducted following standardized protocols, and high-resolution SDS-PAGE was employed to generate reproducible electrophoretic fingerprints. The resulting banding patterns were quantitatively analyzed using GelJ v2 software, and phylogenetic relationships were inferred through cluster analysis. The SDS-PAGE-derived profiles revealed a predominantly conserved proteomic architecture, with overall similarity values ranging from 70% to 100%. Cluster analysis resolved the strains into five principal groups; however, a single dominant cluster encompassed the majority of isolates, indicating a largely homogeneous population structure. Despite this high degree of similarity, subtle yet consistent variations in band intensity and electrophoretic mobility were observed among selected strains, reflecting limited but biologically meaningful phenotypic heterogeneity. Notably, strain-specific deviations in the 35–55 kDa protein region and variations in band intensity, particularly among low-virulence isolates, suggest differential protein expression or possible post-translational modifications rather than large-scale structural proteomic divergence. Importantly, virulence levels exhibited a broad range (0–100%) but did not show a consistent correlation with overall protein profile similarity, indicating that pathogenic variability is likely governed by fine-scale regulatory mechanisms rather than gross proteomic differences. Geographical origin had no discernible impact on clustering patterns, as strains from diverse regions, were interspersed across the dendrogram. This finding suggests extensive epidemiological connectivity and a stable, widely distributed population structure across Türkiye.

Özet

Ateş yanıklığı etmeni olan Erwinia amylovora, yumuşak çekirdekli meyve türlerinde küresel ölçekte önemli ve son derece yıkıcı bir patojen olup, sürdürülebilir tarımsal sistemler üzerinde ciddi ekonomik kısıtlamalar oluşturmaktadır. Bu çalışmada, Türkiye’nin yedi farklı bölgesindeki armut bahçelerinden elde edilen 78 E. amylovora strainin toplam hücresel protein profilleri analiz edilerek fenotipik ilişkilerin ortaya konulması ve popülasyon içi çeşitliliğin değerlendirilmesi amaçlanmıştır. Protein ekstraksiyonu standart protokoller izlenerek gerçekleştirilmiş ve tekrarlanabilir elektroforetik parmak izlerinin elde edilmesi için yüksek çözünürlüklü SDS-PAGE yöntemi kullanılmıştır. Elde edilen bant desenleri GelJ v2 yazılımı kullanılarak kantitatif olarak analiz edilmiş ve filogenetik ilişkiler kümeleme analizi ile belirlenmiştir. SDS-PAGE temelli profiller, %70 ile %100 arasında değişen benzerlik değerleriyle, büyük ölçüde korunmuş bir proteomik yapı ortaya koymuştur. Kümeleme analizi strainleri beş ana gruba ayırmış; ancak tek bir baskın kümenin izolatların büyük çoğunluğunu içermesi, popülasyon yapısının genel olarak homojen olduğunu göstermiştir. Bu yüksek benzerlik düzeyine rağmen, seçilmiş bazı strainler arasında bant yoğunluğu ve elektroforetik hareketlilikte ince fakat tutarlı farklılıklar gözlenmiş, bu durum sınırlı ancak biyolojik açıdan anlamlı bir fenotipik heterojenliğe işaret etmiştir. Özellikle 35–55 kDa protein bölgesinde straine özgü sapmalar ve bant yoğunluğundaki değişimler, özellikle düşük virülenslikli izolatlar arasında, geniş ölçekli yapısal proteomik farklılıklardan ziyade farklı protein ekspresyonu veya olası post-translasyonel modifikasyonları düşündürmektedir. Önemli olarak, virülenslik düzeyleri geniş bir aralıkta (%0–100) değişiklik göstermiş, ancak genel protein profili benzerliği ile tutarlı bir korelasyon ortaya koymamıştır. Bu durum, patojenisite değişkenliğinin büyük ölçekli proteomik farklılıklardan ziyade düzenleyici mekanizmalar tarafından kontrol edildiğini göstermektedir.

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Cite This Article

Gedük, A., Baştaş, K. K. (2026). Linking Total Cell Protein Profiles Revealed by SDS-PAGE in Erwinia amylovora Strains from Pear Orchards in Türkiye to Virulence Level Variation. Journal of Agriculture, Cilt: 9 Sayı: 1. https://doi.org/10.46876/ja.1931758

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