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Journals / International Journal of Chemistry and Technology / 2026 / Volume: 8 Issue: 1

Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant

Osman Bedrettin Akkaya(Kahramanmaraş Sütçü İmam Üniversitesi)0000-0002-8606-881X
İbrahim Seyfettin Çelik(Kahramanmaraş Sütçü İmam Üniversitesi)0000-0001-6946-4477
Erdal Ertaş(KİLİS 7 ARALIK ÜNİVERSİTESİ)0000-0002-4043-5960
Nazan Çömlekcioğlu(Kahramanmaraş Sütçü İmam Üniversitesi)0000-0001-7729-5271
Ashabil Aygan(Kahramanmaraş Sütçü İmam Üniversitesi)0000-0003-4936-9872
Pages
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Publication Date
2024-06-05
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was observed that H. perforatum extracts killed cancer cells at concentrations of 0.1 mg mL-1 and above on H1299, MCF-7 and HUVEC cancer cell lines.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Hypericum perforatum L. (St John's wort), although primarily utilized in traditional medicine, is also frequently employed in modern therapy.
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Key Findings & Data:Following the GC-MS analysis of the extracts, a total of 18 distinct fatty acids were identified.
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Conclusion & Impact:It was observed that H. perforatum extracts killed cancer cells at concentrations of 0.1 mg mL-1 and above on H1299, MCF-7 and HUVEC cancer cell lines.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:1st H1299 · 2nd bioactive compounds · 3rd anticarcinogenic activity

Abstract

Hypericum perforatum L. (St John's wort), although primarily utilized in traditional medicine, is also frequently employed in modern therapy. Within the scope of the study, H. perforatum was collected from Kahramanmaraş region and dried. Extracts derived from above-ground parts were analyzed to determine their levels of total phenolic and flavonoid compounds, as well as their antioxidant, anticancer and antimicrobial properties. Following the GC-MS analysis of the extracts, a total of 18 distinct fatty acids were identified. The main fatty acid components were identified as behenic (37.90 %), linoleic (21.22 %), gamma-linolenic (15.87 %), oleic (9.45 %) and palmitic acid (7.64 %). The plant extracts were found to have a total phenolic content of 60.22 mg GAE g-1 and a flavonoid content of 7.68 µg QE g-1, as determined through analysis. Additionally, FRAP and IC50 values were determined as 26.96 µg AAE g-1 and 0.44 µg mL-1, respectively. The antimicrobial activities of H. perforatum extracts were investigated among a total of 11 microorganisms, including 9 bacteria and 2 yeasts. It has been observed that extracts possess significant antimicrobial activity against all tested microorganisms. H. perforatum extracts were observed to have dose-dependent inhibition of all organisms. It was observed that H. perforatum extracts killed cancer cells at concentrations of 0.1 mg mL-1 and above on H1299, MCF-7 and HUVEC cancer cell lines.

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Cite This Article

Akkaya, O. B., Çelik, İ. S., Ertaş, E., Çömlekcioğlu, N., Aygan, A. (2024). Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant. International Journal of Chemistry and Technology, Volume: 8 Issue: 1. https://doi.org/10.32571/ijct.1445857

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References (49)

  1. Galeotti, N. J. Ethnopharmacology. 2017, 200, 136-146.
  2. Kapoor, S.; Chandel, R.; Kaur, R.; Kumar, S., Kumar, R.; Janghu, S.;. & Kumar, V. Biochem. Syst. Ecol. 2023, 110, 104702.
  3. Seyis, F.; Yurteri, E.; Ozcan, A.; Cirak, C. S. Afr. J. Bot. 2020, 135, 391-403.
  4. Baytop T. Nobel Medical Bookstores. 1999, İstanbul, 166-167.
  5. El-Chaghaby, G. A.; Mohammed, F. S.; Rashad, S.; Uysal, I.; Kocer, O.; Lekesiz, O., Sevindik, M. Egypt. J. Bot. 2024, 64(1), 1-26.
  6. Nobakht, S. Z.; Akaberi, M.; Mohammadpour, A. H.; Moghadam, A. T.; Emami, S. A. Iran. J. Basic Med. Sci. 2022, 25(9), 1045.
  7. Kwiecień, I.; Miceli, N.; Kędzia, E.; Cavò, E.; Taviano, M. F.; Beerhues, L.; Ekiert, H. Molecules. 2023, 28(5), 2376.
  8. Bayram, E.; Geren, H.; Avci, A. B.; Arabaci, O. J. Ege Univ. Fac. Agric. 2004, 41(2).
  9. Ekren, S. J. Agric. Sci. 2010, 16(4).
  10. Filippini, R.; Piovan, A.; Borsarini, A.; Caniato, R. Fitoterapia 2010, 81, 115–119.
  11. Sahebkar-Khorasani, M.; Safarian, M.; Jarahi, L.; Yousefi, M.; Salari, R.; Meshkat, M.; Ayati, M.H.; Bahrami-Taghanaki, H.; Kargozar, R.; Azizi, H. Eur. J. Integr. Med. 2022, 52, 102119.
  12. Kholghi, G.; Arjmandi-Rad, S.; Zarrindast, M.-R.; Vaseghi, S. Naunyn Schmiedebergs Arch. Pharmacol. 2022, 395, 629–642.
  13. Nahrstedt, A.; Butterweck, V. J. Nat. Prod. 2010, 73, 1015–1021.
  14. Linde, K. St. John’s Wort-An overview. Forsch. Komplementarmed. 2009, 16, 146–155.
  15. Davis, P.H. Flora of Turkey and the East Aegean Islands, Edinburgh University. 1965, Press.
  16. Comlekcioglu, N. Braz. Arch. Biol. Technol. 2019, 62, e19180330.
  17. Blainski, A.; Lopes, G.C.; De Mello, J.C.P. Molecules. 2013, 18, 6852-6865. https://doi.org/10.3390/molecules18066852.
  18. Chang, C.C.; Yang, M. H.; Wen, H. M.; Chern, J. C. J Food Drug Anal. 2002, 10 (3): 178-182.
  19. Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Food Sci. Technol. 1995, Volume 28, Issue 1, 1995, Pages 25-30.
  20. Benzie, I. F.; Strain, J. J. AnalBiochem. 1996, 239 (1): 70-76.
  21. NCCLS (National Committee for Clinical Laboratory Standards), 1993.Dilution, Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standards. NCCLS Document. M7-A3.
  22. Collins, C. H.; Lyne, P. M.; Grange, J. M. 1989. Collins and Lyne’s Microbiological Methods, Sixth Edition, Butterworths Co. Ltd. London.
  23. Pelliccia, F.; Marazzi, G.; Greco, C.; Franzoni, F.; Speziale, G.; Gaudio, C. Int. J. Cardiol. 2013, 170: S3- S7.
  24. Jabeur, I.; Pereira, E.; Barros, L.; Calhelha, R. C.; Soković, M.; Oliveira, M. B. P.; Ferreira, I. C. Food Res. Int. 2017, 100: 717-723.
  25. Hosni, K.; Msaâda, K.; Taârit, M. B.; Marzouk, B. Arab. J. Chem. 2013, 10, S2736–S2741.
  26. Bakir, T. K. Turk. J. Agric. Food Sci. Technol. 2018, 6(11): 1647-1652.
  27. Stojanovic, G.; Palic, R.; Tarr, C.H.; Reddy, C.M.; Marinkovic, O. Biochem. Syst. Ecol. 2003, 31, 223–226.
  28. Sevindik M. Mycopath. 2018, 16(2): 47-49.
  29. Mohammed, F. S.; Sabik, A. E.; Dogan, M.; Selamoglu, Z., Sevindik, M. Bull. Biotechnol. 2020, 1(2), 43-45.
  30. Raziq, N.; Muhammad, N.; Chishti, K. A.; Saeed, M.; Rahman, S.; Khan, H. Afr. J. Pharm. Pharmacol. 2011, Vol. 5(16), pp. 1872-1876.
  31. Piatti, D.; Marconi R.; Caprioli, G.; Angeloni, S.; Ricciutelli, M.; Zengin, G.; Maggi, F.; Pagni, L.; Sagratini, G. L. Appl. Sci. 2023, 13, 13304.
  32. Mahomoodally, M.F.; Zengin, G.; Roumita, S.; Caprioli, G.; Mustafa, A.M.; Piatti, D.; Yıldıztugay, E.; Ak, G.; Karada˘g, A.E.; Khalid, A.; et al. Chem. Biodivers. 2023, 20, e202201181.
  33. Napoli, E.; Siracusa, L.; Ruberto, G.; Carrubba, A.; Lazzara, S.; Speciale, A.; Cimino, F.; Saija, A.; Cristani, M. Phytochemistry. 2018, 152, 162–173.
  34. Alahmad, A.; Alghoraibi, I.; Zein, R.; Kraft, S.; Dräger, G.; Walter, J.G.; Scheper, T. ACS Omega. 2022, 7, 13475–13493.
  35. Ozturk, N.; Tuncel, M.; Poto˘glu-Erkara, ˙I. Pharm. Biol. 2009, 47, 120–127.
  36. Lourenco, S.C.; Moldão-Martins, M.; Alves, V.D. Molecules. 2019, 24, 4132.
  37. Conforti, F.; Statti, G. A., Tundis, B.; Bianchi, A.; Agrimonti, C.; Sacchetti, G.; Andreotti, E.; Menichini, F.; Poli, F. Nat. Prod. Res. 2005, 19(3), 295,303.
  38. Agnol, R. D.; Ferraz, A.; Bernardi, A. P.; Albring, D.; Nor, Sarmento, C. L.; Lamb, L., Hass, M.; Poser, G.; Schapoval, E. S. Phytomedicine. 2003, 10, 511–516.
  39. Torre, L. A.; Siegel, R. L.; Ward, E. M.; Jemal, A. Cancer Epidemiol Biomarkers Prev. 2016, 25 (1): 16–27. https://doi.org/10.1158/1055-9965.EPI-15-0578.
  40. Karakas, N.; Okur, M. E.; Ozturk, I.; Ayla, S.; Karadag, A. E., Polat, D. C. Medeni Med J. 2019, 34(3):297-304.
  41. Nair, H. K.; Rao, K.V.; Aalinkeel, R.; Mahajan, S., Chawda, R.; Schwartz, S. A. Clin. Diagn. Lab Immunol. 2004, 11 63–69.
  42. Matic, I. Z.; Ergun S.; Crnogorac M. Đ.; Misir S., Aliyazicioglu Y., Damjanović A.; Džudžević-Čančar H., Stanojković T.; Konanc K., Petrović N. Cytotechnology. 2021, 73: 373–389.
  43. Kapoor, S.; Chandel, R.; Kaur, R.; Kumar, S., Kumar, R.; Janghu, S.;. & Kumar, V. Biochem. Syst. Ecol. 2023, 110, 104702.
  44. Seyis, F.; Yurteri, E.; Ozcan, A.; Cirak, C. S. Afr. J. Bot. 2020, 135, 391-403.
  45. Nobakht, S. Z.; Akaberi, M.; Mohammadpour, A. H.; Moghadam, A. T.; Emami, S. A. Iran. J. Basic Med. Sci. 2022, 25(9), 1045.
  46. Ekren, S. J. Agric. Sci. 2010, 16(4).
  47. Comlekcioglu, N. Braz. Arch. Biol. Technol. 2019, 62, e19180330.
  48. Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Food Sci. Technol. 1995, Volume 28, Issue 1, 1995, Pages 25-30.
  49. Conforti, F.; Statti, G. A., Tundis, B.; Bianchi, A.; Agrimonti, C.; Sacchetti, G.; Andreotti, E.; Menichini, F.; Poli, F. Nat. Prod. Res. 2005, 19(3), 295,303.