Journals / Clinical Dentistry and Research / 2016 / Cilt: 40 - Sayı: 1

BIOFILM FORMATION AND CYTOTOXICITY OF THREE DIFFERENT DENTAL CERAMIC MATERIALS

ÜÇ FARKLI DENTAL SERAMİK MATERYALİN BIYOFİLM OLUŞTURMASI VE SİTOTOKSİSİTESİ

Pages
11–18
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The Pearson Correlation test did not reveal a linear correlation between biofilm formation and fibroblast survival after both 24 and 48 hours.Conclusion: All the dental materials used in this study induced cytotoxic reactions.

📌 3 Key Takeaways

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Objective & Hypothesis:Background and Aim: Ceramic materials are widely used in dental practice because of their many beneficial properties like biological and mechanical features and aesthetics.
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Key Findings & Data:However as the structure and surface characteristics of these restorations change the clinical outcomes may be altered.Materials and methods: Disc shaped dental ceramic specimens with 0.5 mm thickness and 2 mm radius were fabricated from feldspathic ceramic (n=50), lithium di-silicate based ceramic (n=50) and yttrium stabilized zirconia based ceramic (n=50).
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Conclusion & Impact:The Pearson Correlation test did not reveal a linear correlation between biofilm formation and fibroblast survival after both 24 and 48 hours.Conclusion: All the dental materials used in this study induced cytotoxic reactions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Background and Aim: Ceramic materials are widely used in dental practice because of their many beneficial properties like biological and mechanical features and aesthetics. However as the structure and surface characteristics of these restorations change the clinical outcomes may be altered.Materials and methods: Disc shaped dental ceramic specimens with 0.5 mm thickness and 2 mm radius were fabricated from feldspathic ceramic (n=50), lithium di-silicate based ceramic (n=50) and yttrium stabilized zirconia based ceramic (n=50). Four different microorganisms were selected for this study for biofilm formation evaluation. Mouse fibroblast cells (McCoy cells) were used for the in vitro cytotoxicity tests. Optical density (OD) of stained adherent bacteria was determined with a micro ELISA auto reader at wavelength of 620 nm spectrophotometrically. One-way ANOVA, Post Hoc tests, Independent Sample-T tests, Kruskall-Wallis test and the Pearson Correlation tests were used for statistical analysis.Results: The survival of fibroblast cells at Lithium di-silicate based ceramic group (IPS) was the highest after 24 hours. Feldspathic group exhibited the lowest mean optical density after 48 hours. The Pearson Correlation test did not reveal a linear correlation between biofilm formation and fibroblast survival after both 24 and 48 hours.Conclusion: All the dental materials used in this study induced cytotoxic reactions.

Özet

Amaç: Seramik materyaller biyolojik, mekanik ve estetik gibi pek çok yararlı özelliği sayesinde Diş Hekimliği pratiğinde yaygın olarak kullanılır. Ancak yapısı ve yüzey karakteristiği değiştikçe klinik sonuçlar etkilenebilir.Metod: Feldspatik seramikten (n= 50), lityum-disilikat bazlı seramikten (n= 50) ve yitrium-stabilize zirkonya seramikten (n= 50) 0.5 mm kalınlıkta ve 2 mm yarıçaplı disk şeklinde örnekler hazırlandı. Bu çalışmada, biyofilm değerlendirmesi için 4 farklı mikroorganizma seçildi. Sitotoksisite testi için fare fibroblast hücreleri (McCoy cells) kullanıldı. Yüzeye yapışan mikroorganizma boyanmalarının optik yoğunluğu mikroeliza ile 620 nm dalgaboyunda spektrofotometrik olarak ölçüldü. Tek yönlü ANOVA, Post-hoc testleri, bağımsız T testleri, kruskall-Wallis ve Pearson Korelasyon testi kullanılarak istatistiksel analizler yapıldı.Bulgular: Lityum-disilikat (IPS) bazlı seramik grubunda bulunan hücreler 24 saat sonra en yüksek yaşam değerlerini gösterdi. Feldspatik grup en düşük optik yoğunluk değerleri ortaya çıkardı. Pearson korelasyon testi 24 ve 48 saat sonra biyofilm oluşumu ve fibroblast yaşam değerleri arasında doğrusal bir korelasyon göstermedi.Sonuç: Bu çalışmada kullanılan bütün dental materyaller sitotoksik reaksiyon ortaya çıkardı.

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Cite This Article

ŞAHİN, C., KEYF, F., UZUN, G., ÜNSAL, A. (2016). BIOFILM FORMATION AND CYTOTOXICITY OF THREE DIFFERENT DENTAL CERAMIC MATERIALS. Clinical Dentistry and Research, Cilt: 40 - Sayı: 1, 11–18. https://ajindex.com/articles/146338

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References (12)

  1. Williams DF. On the mechanisms of biocompatibility. Biomaterials; 29: 2941-2953.
  2. Napankangas R, Raustia A. Twenty-year follow-up of metal- ceramic single crowns: a retrospective study. The International journal of prosthodontics 2008; 21: 307-311.
  3. Liu PR, Essig ME. Panorama of dental CAD/CAM restorative systems. Compend Contin Educ Dent 2008; 29: 482-8
  4. Miyazaki T, Nakamura T, Matsumura H, Ban S, Kobayashi T. Current status of zirconia restoration. Journal of prosthodontic research. 2013; 57: 236-261. Anusavice KJ. Degradability of dental ceramics. Advances in dental research 1992; 6: 82-89.
  5. Guo Y, Chen D, Lu W, Jia Y, Wang L, Zhang X. Corrosion resistance and in vitro response of a novel Ti35Nb2Ta3Zr alloy with a low Young's modulus. Biomed Mater 2013; 8: 055004. Rosalbino F, Maccio D, Giannoni P, Quarto R, Saccone A. Study of the in vitro corrosion behavior and biocompatibility of Zr-2.5Nb and Zr-1.5Nb-1Ta (at%) crystalline alloys. Journal of materials science Materials in medicine 2011; 22: 1293-1302.
  6. Kurella A, Dahotre NB. Laser induced multi-scale textured zirconia coating on Ti-6Al-4V. Journal of materials science Materials in medicine 2006; 17: 565-572.
  7. Abduo J, Lyons K, Swain M. Fit of zirconia fixed partial denture: a systematic review. J Oral Rehabil 2010; 37: 866-876.
  8. Hickel R, Manhart J. Longevity of restorations in posterior teeth and reasons for failure. The journal of adhesive dentistry 2001; 3: 64. Manhart J, Chen H, Hamm G, Hickel R. Buonocore Memorial Lecture. Review of the clinical survival of direct and indirect restorations in posterior teeth of the permanent dentition. Operative dentistry 2004; 29: 481-508.
  9. Wang YX, Robertson JL, Spillman WB, Jr., Claus RO. Effects of the chemical structure and the surface properties of polymeric biomaterials on their biocompatibility. Pharmaceutical research;21:1362-73.
  10. Sahin C, Ergin A, Ayyildiz S, Cosgun E, Uzun G. Effect of biofilm formation, and biocorrosion on denture base fractures. J Adv Prosthodont 2013; 5: 140-46.
  11. Rutkunas V, Bukelskiene V, Sabaliauskas V, Balciunas E, Malinauskas M, Baltriukiene D. Assessment of human gingival fibroblast interaction with dental implant abutment materials. Journal of materials science Materials in medicine 2015; 26: 169. Baek KJ, Choi Y, Ji S. Gingival fibroblasts from periodontitis patients exhibit inflammatory characteristics in vitro. Archives of oral biology 2013; 58: 1282-1292.
  12. Kokoti M, Sivropoulou A, Koidis P, Garefis P. Comparison of cell proliferat