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Journals / Animal Health Production and Hygiene / 2024 / Cilt: 13 Sayı: 2

Molecular Typing of Vibrio species Isolated from Seabass (Dicentrarchus labrax) and Detection of Antibiotic Resistance

Oğuzhan Dolgun0000-0002-9681-6500
Şükrü Kırkan(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0001-5111-8656
Hafize Tuğba Yüksel Dolgun(AYDIN ADNAN MENDERES ÜNİVERSİTESİ)0000-0002-1125-5792
Pages
9–20
Publication Date
2024-12-30
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, it was determined that Vibrio species have an important role as a primary agent in fish diseases, molecular methods give more reliable results in identification, and there is single and multiple antibiotic resistance among isolates.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study was conducted to isolate Vibrio species from sea bass, identify them using molecular methods, and determine their antibiotic resistance.
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Key Findings & Data:In the research, 100 sea bass samples taken from fish farms in the Aegean region between May and September 2021 were examined.
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Conclusion & Impact:In this study, it was determined that Vibrio species have an important role as a primary agent in fish diseases, molecular methods give more reliable results in identification, and there is single and multiple antibiotic resistance among isolates.
🔬Design:Araştırma Makalesi
📍Scope:2021 Verileri
⚙️Variables:Antibiotic resistance · identification · PCR

Abstract

This study was conducted to isolate Vibrio species from sea bass, identify them using molecular methods, and determine their antibiotic resistance. In the research, 100 sea bass samples taken from fish farms in the Aegean region between May and September 2021 were examined. After the phenotypic and genotypic identification of the isolates obtained from the samples, antibiotic resistances were determined by the disk diffusion method and antibiotic resistance genes were determined by multiplex PCR. In this study, 46 (46%) Vibrio spp. isolates were obtained from 100 sea bass samples by conventional and biochemical methods. The obtained 46 isolates were confirmed to be Vibrio spp. by 16S rRNA PCR. From 46 isolates, 22 (47,8%) isolates were identified as V. alginolyticus, 13 (28,2%) isolates as V. harveyi, 3 (6,5%) isolates as V. parahaemolyticus, 1 (2%) isolate as V. vulnificus and 7 (15%) isolates as Vibrio spp. by multiplex PCR. The highest resistance was found to ampicillin (84,8%) in the isolates in the antibiogram. All isolates were found to be susceptible to enrofloxacin and sulfamethoxazole-trimethoprim (100%). In isolates, the highest resistance gene was found to be trimethoprim resistance gene (63%), and the lowest resistance gene was found to be fluoroquinolone resistance gene (6,5%). In this study, it was determined that Vibrio species have an important role as a primary agent in fish diseases, molecular methods give more reliable results in identification, and there is single and multiple antibiotic resistance among isolates.

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Cite This Article

Dolgun, O., Kırkan, Ş., Dolgun, H. T. Y. (2024). Molecular Typing of Vibrio species Isolated from Seabass (Dicentrarchus labrax) and Detection of Antibiotic Resistance. Animal Health Production and Hygiene, Cilt: 13 Sayı: 2, 9–20. https://doi.org/10.53913/aduveterinary.1528574

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