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Journals / Animal Health Production and Hygiene / 2026 / Cilt: 15 Sayı: 2

Effects of Locally Applied Clove and Green Tea Hydrosols on Cyclophosphamide-Induced Oral Mucositis in Rats

Ratlarda Siklofosfamid ile İndüklenen Oral Mukozitte Lokal Olarak Uygulanan Karanfil ve Yeşil Çay Hidrosollerinin Etkileri

Emine Tarlabelen Karaytuğ(ADNAN MENDERES UNIVERSITY, FACULTY OF NURSING)0000-0002-4892-5040
Sakine Boyraz Özkavak(Aydın Adnan Menderes University Nursing Faculty)0000-0001-9699-6495
Serap Gökçe Eskin(Aydın Adnan Menderes University Nursing Faculty)0000-0002-8940-6116
Pages
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Publication Date
2026-05-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It is recommended that studies be conducted to investigate the effect of clove and green tea hydrosols on preventing oral mucositis in patients.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study investigated the effect of clove and green tea hydrosol applied locally in a rat model of oral mucositis induced with cyclophosphamide.
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Key Findings & Data:The study was conducted using 40 female Wistar albino rats and were randomly divided into 8 groups.
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Conclusion & Impact:It is recommended that studies be conducted to investigate the effect of clove and green tea hydrosols on preventing oral mucositis in patients.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Karanfil · Oral mukozit · Siklofosfamid

Abstract

This study investigated the effect of clove and green tea hydrosol applied locally in a rat model of oral mucositis induced with cyclophosphamide. This study is a preclinical, in vivo, experimental, and analytical study. The study was conducted using 40 female Wistar albino rats and were randomly divided into 8 groups. A single high-dose cyclophosphamide injection was administered intraperitoneally for mucositis induction. For mucositis care, the rats received twice daily oral spray clove or green tea hydrosol in the experimental groups. These interventions continued until collection of cardiac blood samples and oral mucosal tissue samples from the right cheek on day 4 or day 8. Serum total antioxidant status (TAS) and total oxidant status (TOS) were determined from the blood samples, and the tissue samples were analyzed for erosion, fibrosis, and inflammatory cell infiltration. Mean serum TAS was higher and TOS was lower in the clove and green tea hydrosol groups. There was also significantly less erosion in the clove and green tea hydrosol groups than in the positive control groups (P=0.003). On day 8, the clove and green tea hydrosol groups had less fibrosis and showed minimal inflammatory cell infiltration. In rats, clove and green tea hydrosols were found to be effective in preventing erosion associated with cyclophosphamide-induced oral mucositis. It is recommended that studies be conducted to investigate the effect of clove and green tea hydrosols on preventing oral mucositis in patients.

Özet

Bu araştırmada, ratlarda siklofosfamid ile oluşturulan oral mukozit modelinde lokal uygulanan karanfil ve yeşil çay hidrosolünün etkisi araştırılmıştır. Bu çalışma, preklinik, in vivo deneysel ve analitik bir araştırmadır. Çalışma, 40 dişi Wistar albino rat kullanılarak yürütülmüş ve ratlar rastgele 8 gruba ayrılmıştır. Mukozit indüksiyonu için ratlara intraperitoneal olarak tek yüksek dozda (300 mg/kg) siklofosfamid uygulanmıştır. Mukozit bakımını sağlamak için deney gruplarındaki ratlara günde iki kez karanfil veya yeşil çay hidrosolü ağız spreyi şeklinde uygulanmıştır. Bu uygulamalara kalp kanı ve sağ yanak mukozasından doku örneklerinin alındığı deneyin 4. gün ve 8. gününe kadar devam edilmiştir. Toplanan kan örneklerinden serum toplam antioksidan seviyesi (TAS) ve toplam oksidan seviyesi (TOS) ölçülmüş, doku örnekleri ise erozyon, fibrozis ve inflamatuar hücre infiltrasyonu açısından incelenmiştir. Karanfil ve yeşil çay hidrosolü uygulanan gruplarda serum TAS ortalama değeri daha yüksek, TOS değeri ise daha düşüktü. Ayrıca pozitif kontrol gruplarına kıyasla, karanfil ve yeşil çay hidrosolü uygulanan gruplarda erozyon istatistiksel olarak anlamlı derecede (P=0,003) daha az gelişti. Sekizinci gün karanfil ve yeşil çay hidrosolü gruplarında fibrozis daha az ve inflamatuar hücre infiltrasyonu minimum seviyedeydi. Ratlarda, siklofosfamide bağlı gelişen oral mukozitte karanfil ve yeşil çay hidrosolünün erozyonu önlemede etkili olduğu saptanmıştır. Karanfil ve yeşil çay hidrosolünün hastalarda oral mukoziti önlemeye yönelik etkisinin çalışmalarla araştırılması önerilmektedir.

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Cite This Article

Karaytuğ, E. T., Özkavak, S. B., Eskin, S. G. (2026). Effects of Locally Applied Clove and Green Tea Hydrosols on Cyclophosphamide-Induced Oral Mucositis in Rats. Animal Health Production and Hygiene, Cilt: 15 Sayı: 2. https://doi.org/10.53913/aduveterinary.1822967

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