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Journals / Journal of advances in vetbio science and techniques / 2026 / Volume: 9 Issue: 2

Investigation of cytogenetic, electrophoretic, histopathological and biochemical effects of walnut (juglans regia l.) Leaf extract in rats experimentally induced diabetes by streptozotocin

Pinar Aksu Kılıçle(KAFKAS UNIVERSITY)0000-0002-3567-5775
Evren Koç(KAFKAS UNIVERSITY)0000-0002-0022-9433
Ebru Karadağ Sarı(KAFKAS UNIVERSITY)0000-0002-5917-9565
Abdullah Doğan(KAFKAS UNIVERSITY)0000-0003-2008-6713
Hasan Asker(Uşak Üniversitesi)0000-0002-5703-2164
Yağmur Yıldız Asker(KAFKAS UNIVERSITY)0000-0003-0635-0098
Pages
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Publication Date
2024-08-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result, it was concluded that the application of JR leaf extract may have a protective effect against damage caused by diabetes.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, the effects of Juglans regia L. (JR) leaf extract on histological damage and cytogenetic, electrophoretic, and biochemical parameters in the liver and kidney tissues of diabetic rats were investigated.
📊
Key Findings & Data:In the study, 60 male rats (Sprague-Dawley) were separated into six groups.
💡
Conclusion & Impact:As a result, it was concluded that the application of JR leaf extract may have a protective effect against damage caused by diabetes.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Diabetes · Juglans regia L. · kidney

Abstract

In this study, the effects of Juglans regia L. (JR) leaf extract on histological damage and cytogenetic, electrophoretic, and biochemical parameters in the liver and kidney tissues of diabetic rats were investigated. In the study, 60 male rats (Sprague-Dawley) were separated into six groups. According to sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) data, it was determined that there were increases and decreases in different serum protein expressions because of the treatment of JR leaf extract in the diabetes group. In the kidney tissue of the diabetes group, an increase in volume in the glomeruli and narrowing of the Bowman's space as well as thickening of the walls of tubules were detected. Vacuolization and shedding were observed in the epithelial cells of tubules in the cortical regions in kidney. In diabetic + JR extract groups administered JR leaf extract at doses of 250 mg/kg and 500 mg/kg, serum AST and ALT levels were reduced compared to the diabetic group. Diabetic rats' livers showed spotted necrosis and fibrosis in the portal area, biliary tract proliferation, mild inflammation, increased vascularization, unicellular necrosis in hepatocytes, and sinusoidal dilatation. The JR leaf extract group did not exhibit these problems. JR leaf extract influences reducing hepatotoxic and oxidative damage due to diabetes and increasing the level of antioxidant enzymes. As a result, it was concluded that the application of JR leaf extract may have a protective effect against damage caused by diabetes.

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Cite This Article

Kılıçle, P. A., Koç, E., Sarı, E. K., Doğan, A., Asker, H., Asker, Y. Y. (2024). Investigation of cytogenetic, electrophoretic, histopathological and biochemical effects of walnut (juglans regia l.) Leaf extract in rats experimentally induced diabetes by streptozotocin. Journal of advances in vetbio science and techniques, Volume: 9 Issue: 2. https://doi.org/10.31797/vetbio.1454981

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References (48)

  1. Arora, S., Brits, E., Kaur, S., Kaur, K., Sohi, R. S., Kumar, S., & Verschaeve, L. (2005). Evaluation or genotoxicity of medicinal plant extracts by the comet and VITOTOX (R) tests. Journal of Environmental Pathology Toxicology and Oncology, 24(3), 193-200. https://doi.org/10.1615/JEnvPathToxOncol.v24.i3.50
  2. Asokan, S. M., Wang, T., Su, W. T., & Lin, W. T. (2019). Antidiabetic effects of a short peptide of potato protein hydrolysate in STZ-Induced diabetic mice. Nutrients, 11(4), 779. https://doi.org/10.3390/nu11040779
  3. Aydin, S., Gokce, Z., & Yilmaz, O. (2015). The effects of Juglans regia L. (walnut) extract on certain biochemical paramaters and in the prevention of tissue damage in brain, kidney, and liver in CCl4 applied Wistar rats. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi, 40(3), 241-250. https://doi.org/10.1515/tjb-2015-0009
  4. Caylak, E., & Nur, G. (2024a). In vitro characterisation of antioxidant, anti-aging, anti-alzheimer, antiobesity, antidiabetic activities of silver nanoparticles synthesised from salvia willeana. Ann Clin Anal Med, 15(Suppl 1), S1-7. https://doi.org/10.4328/ACAM.22120
  5. Caylak, E., & Nur, G. (2024b). In vitro DNA damage prevention, antioxidant and antidiabetic activities of Achillea biebersteinii/millefolium plant extracts and synthesized ZnO nanoparticles. Ann Clin Anal Med, 15(2), 136-140. https://doi.org/10.4328/ ACAM.22057
  6. Cerda, B., Tomas-Barberan, F. A., & Espin, J. C. (2005). Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans: Identification of biomarkers and individual variability. Journal of Agricultural and Food Chemistry, 53(2), 227-235. https://doi.org/10.1021/jf049144d
  7. Çelik, Ö., & Koç, E. (2019). Rumex crispus extract exerts anti-diabetic properties in streptozotocin-induced diabetes in rats. Journal of the Institute of Science and Technology, 9(4), 1937-1943. https://doi.org/10.21597/jist.585885
  8. Deprem, T., Yıldız, S. E., Sarı, E. K., Bingöl, S. A., Taşçı, S. K., Aslan, Ş., Sözmen, M., & Nur, G. (2015). Distribution of glutathione peroxidase 1 in liver tissues of healthy and diabetic rats treated with capsaicin. Biotechnic&Histochemistry, 90(1), 1-7. https://doi.org/10.3109/10520295.2014.919024
  9. Deveci, H. A., Nur, G., & Aksu Kılıçle, P. (2021). Subakut malathion uygulamasının oksidatif stres biyobelirteçlerine etkisi. Journal of Advances in VetBio Science and Techniques, 6(3), 193-201. https://doi.org/10.31797/vetbio.917112
  10. Eidi, A., Moghadam, J. Z., Mortazavi, P., Rezazadeh, S., & Olamafar, S. (2013). Hepatoprotective effects of Juglans regia extract against CCl4-induced oxidative damage in rats. Pharmaceutical Biology, 51(5), 558-565. https://doi.org/10.3109/13880209.2012.749920
  11. Eisenthal, R., & Danson, M. J. (1993). Enzyme assays. Oxford University Press.
  12. Erel, O. (2004). A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clinical Biochemistry, 37(4), 277-285. https://doi.org/10.1016/j.clinbiochem.2003.11.015
  13. Forino, M., Stiuso, P., Lama, S., Ciminiello, P., Tenore, G. C., Novellino, E., & Taglialatela-Scafati, O. (2016). Bioassay-guided identification of the antihyperglycaemic constituents of walnut (Juglans regia) leaves. Journal of Functional Foods, 26, 731-738. https://doi.org/10.1016/j.jff.2016.08.053
  14. Ghiravani, Z., Hosseini, M., Taheri, M. M. H., Fard, M. H., & Abedini, M. R. (2016). Evaluation of hypoglycemic and hypolipidemic effects of internal septum of walnut fruit in alloxan-induced diabetic rats. African Journal of Traditional Complementary and Alternative Medicines, 13(2), 94-100. https://doi.org/10.4314/ajtcam.v13i2.12
  15. Giacco, F., & Brownlee, M. (2010). Oxidative stress and diabetic complications. Circulation Research, 107(9), 1058-1070. https://doi.org/10.1161/Circresaha.110.223545
  16. Gundogdu, M., Akdeniz, F., Ozbek, F. E., Demirci, S., & Adiguzel, V. (2016). A promising method for recovery of oil and potent antioxidant extracts from Pistacia khinjuk Stocks seeds. Industrial Crops and Products, 83, 515-521. https://doi.org/10.1016/j.indcrop.2015.12.076
  17. Halvorsen, B. L., Holte, K., Myhrstad, M. C. W., Barikmo, I., Hvattum, E., Remberg, S. F., Wold, A. B., Haffner, K., Baugerod, H., Andersen, L. F., Moskaug, J. O., Jacobs, D. R., & Blomhoff, R. (2002). A systematic screening of total antioxidants in dietary plants. Journal of Nutrition, 132(3), 461-471. https://doi.org/10.1093/jn/132.3.461
  18. Hoogwerf, B. J. (2020). Type of diabetes mellitus: Does it matter to the clinician? Cleveland Clinic Journal of Medicine, 87(2), 100-108. https://doi.org/10.3949/ccjm.87a.19020
  19. Hosseini, S. A., Mohammadi, J., Delaviz, H., & Shariati, M. (2018). Effect of Juglans regia and Nasturtum officinalis biochemical parameters following toxicity of kidney by CCl4 in Wistar rats. Electronic Journal of General Medicine, 15(3), 1-7. https://doi.org/10.29333/ejgm/86193
  20. Konukoglu, D. (2008). Properties, functions of omega-3 and omega-6 fatty acids and relationship between essential fatty acids and cardiovascular diseases. Turkish Journal of Family Practice, 12, 121-129. https://doi.org/10.2399/tahd.08.121
  21. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259), 680-685. https://doi.org/10.1038/227680a0
  22. Li, C. S., Wang, Z. H., Li, X. L., & Chen, J. (2019). Effects of semicarbazide-sensitive amine oxidase inhibitors on morphology of aorta and kidney in diabetic rats. Bmc Endocrine Disorders, 19. https://doi.org/10.1186/s12902-019-0392-1
  23. Lipinski, B. (2001). Pathophysiology of oxidative stress in diabetes mellitus. Journal of Diabetes and Its Complications, 15(4), 203-210. https://doi.org/10.1016/S1056-8727(01)00143-X
  24. Liu, Y., Li, L., Yi, B., Hu, Z. X., Li, A. M., Yang, C., Zheng, L., & Zhang, H. (2019). Activation of vitamin D receptor attenuates high glucose-induced cellular injury partially dependent on CYP2J5 in murine renal tubule epithelial cell. Life Sciences, 234. https://doi.org/10.1016/j.lfs.2019.116755
  25. Luna, L. G. (1968). Manual of histologic staining methods of the armed forces institute of pathology (3rd ed.). McGraw-Hill. Book Co.
  26. Memisogullari, R. (2005). The role of free radicals and the effect of antioxidants in diabetes. Duzce Medical Journal, 7(3), 30-39.
  27. Mohammadi, J., Saadipour, K., Delaviz, H., & Mohammadi, B. (2011). Anti-diabetic effects of an alcoholic extract of Juglans regia in an animal model. Turkish Journal of Medical Sciences, 41(4), 685-691. https://doi.org/10.3906/sag-1004-802
  28. Mollica, A., Zengin, G., Locatelli, M., Stefariucci, A., Macedonio, G., Bellagamba, G., Onaolapo, O., Onaolapo, A., Azeez, F., Ayileka, A., & Novellino, E. (2017). An assessment of the nutraceutical potential of Juglans regia L. leaf powder in diabetic rats. Food and Chemical Toxicology, 107, 554-564. https://doi.org/10.1016/j.fct.2017.03.056
  29. Muzaffer, U., Paul, V. I., Prasad, N. R., & Karthikeyan, R. (2018). Juglans regia L. protects against UVB induced apoptosis in human epidermal keratinocytes. Biochemistry and Biophysics Reports, 13, 109-115. https://doi.org/10.1016/j.bbrep.2018.01.004
  30. Nur, G., Caylak, E., Deveci, H. A., Aksu Kilicle, P., & Deveci A. (2023). The protective effect of caffeic acid phenethyl ester in the nephrotoxicity induced by α-cypermethrin. Open Medicine, vol. 18, no. 1, pp. 20230781. https://doi.org/10.1515/med-2023-0781.
  31. O'Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. The Journal of Biological Chemistry, 250(10), 4007-4021. https://doi.org/10.1016/S0021-9258(19)41496-8
  32. Ozer, O., Mutlu, B., & Kicvak, B. (2007). Antityrosinase activity of some plant extracts and formulations containing ellagic acid. Pharmaceutical Biology, 45(6), 519-524. https://doi.org/10.1080/13880200701446746
  33. Pereira, J. A., Oliveira, I., Sousa, A., Valentao, P., Andrade, P. B., Ferreira, I. C. F. R., Ferreres, F., Bento, A., Seabra, R., & Estevinho, L. (2007). Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food and Chemical Toxicology, 45(11), 2287-2295. https://doi.org/10.1016/j.fct.2007.06.004
  34. Preston, R. J., Dean, B. J., Galloway, S., Holden, H., McFee, A. F., & Shelby, M. (1987). Mammalian in vivo cytogenetic assays. Analysis of chromosome aberrations in bone marrow cells. Mutation Research, 189(2), 157-165. https://doi.org/10.1016/0165-1218(87)90021-8
  35. Rabiei, K., Ebrahimzadeh, M. A., Saeedi, M., Bahar, A., Akha, O., & Kashi, Z. (2018). Effects of a hydroalcoholic extract of Juglans regia (walnut) leaves on blood glucose and major cardiovascular risk factors in type 2 diabetic patients: a double-blind, placebo-controlled clinical trial. BMC Complement Altern Med, 18(1), 206. https://doi.org/10.1186/s12906-018-2268-8
  36. Rusu, M. E., Georgiu, C., Pop, A., Mocan, A., Kiss, B., Vostinaru, O., Fizesan, I., Stefan, M. G., Gheldiu, A. M., Mates, L., Moldovan, R., Muntean, D. M., Loghin, F., Vlase, L., & Popa, D. S. (2020). Antioxidant effects of walnut (Juglans regia L.) kernel and walnut septum extract in a D-galactose-induced aging model and in naturally aged rats. Antioxidants, 9(5). https://doi.org/10.3390/antiox9050424
  37. Saxena, A. K., Srivastava, P., Kale, R. K., & Baquer, N. Z. (1993). Impaired antioxidant status in diabetic rat-liver - effect of vanadate. Biochemical Pharmacology, 45(3), 539-542. https://doi.org/10.1016/0006-2952(93)90124-F
  38. Schmid, W. (1975). The micronucleus test. Mutation Research, 31(1), 9-15. https://doi.org/10.1016/0165-1161(75)90058-8
  39. Sharma, P., Verma, P. K., Sood, S., Pankaj, N. K., Agarwal, S., & Raina, R. (2021). Neuroprotective potential of hydroethanolic hull extract of Juglans regia L. on isoprenaline induced oxidative damage in brain of Wistar rats. Toxicology Reports, 8, 223-229. https://doi.org/10.1016/j.toxrep.2021.01.006
  40. Stampar, F., Solar, A., Hudina, M., Veberic, R., & Colaric, M. (2006). Traditional walnut liqueur - cocktail of phenolics. Food Chemistry, 95(4), 627-631. https://doi.org/10.1016/j.foodchem.2005.01.035
  41. Tapsell, L. C., Gillen, L. J., Patch, C. S., Batterham, M., Owen, A., Bare, M., & Kennedy, M. (2004). Including walnuts in a low-fat/modified-fat diet improves HDL cholesterol-to-total cholesterol ratios in patients with type 2 diabetes. Diabetes Care, 27(12), 2777-2783. https://doi.org/10.2337/diacare.27.12.2777
  42. Uluman, E., & Aksu-Kılıçle, P.(2020). The investigation of the possible antigenotoxic in vivo effects of pomegranate (Punica granatum L.) peel extract on mitomycin-C genotoxicity. Turkish Journal of Veterinary & Animal Sciences, 44(2), 382-390. https://doi.org/10.3906/vet-1911-79
  43. Weber, K., Pringle, J. R., & Osborn, M. (1972). Measurement of molecular weights by electrophoresis on SDS-acrylamide gel. Methods Enzymol, 26, 3-27. https://doi.org/10.1016/s0076-6879(72)26003-7
  44. Yaman, T., & Doğan, A. (2016). Streptozotosin ile diyabet oluşturulan sıçanlarda meşe palamudu (Quercus branti lindl.) ekstraktların karaciğer ve pankreası koruyucu etkileri. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 1(2), 7-15.
  45. Yapislar, H., Haciosmanoglu, E., Sarioglu, T., Degirmencioglu, S., Sogut, I., Poteser, M., & Ekmekcioglu, C. (2022). Anti-inflammatory effects of melatonin in rats with induced type 2 diabetes mellitus. Life (Basel), 12(4). https://doi.org/10.3390/life12040574
  46. Yıldız, S. E., Deprem, T., Sarı, E. K., Bingöl, S. A., Taşçı, S. K., Aslan, Ş., Nur, G., & Sözmen, M. (2015). Immunohistochemical distrubition of leptin in kidney tissues of melatonin treated diabetic rats. Biotechnic&Histochemistry, 90(4), 270-277. https://doi.org/10.3109/10520295.2014.983548
  47. Yiğit, D., Yiğit, N., Aktaş, E., & Özgen, U. (2009). Antimicrobial activity of walnut (Juglans Regia L.). Turkish Microbiological Society, 39(1-2), 7-11.
  48. Yoshikawa, T., Toyokuni, S., Yamamoto, Y., & Naito, Y. (2000). Free radicals in chemistry biology and medicine. OICA International (UK) Limited.