SavedAdd your journal

Journals / Journal of advances in vetbio science and techniques / 2026 / Volume: 10 Issue: 1

Complications and therapeutic approaches in a sciatic nerve injury rat model

Volga Öztürk(Manisa Turgutlu Hospital, Orthopedics and Traumatology, Türkiye)0009-0009-7195-3435
Ali Engin Dastan(UNIVERSITY OF HEALTH SCIENCES, FACULTY OF MEDİCİNE, MEDICINE PR.)0000-0003-3637-8550
Yasemen Adalı Ruşen(IZMIR DEMOCRACY UNIVERSITY)0000-0002-8004-7364
Elif Barış(İZMİR EKONOMİ ÜNİVERSİTESİ)0000-0001-6838-7932
Pages
—
Publication Date
2025-04-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

These findings suggest the need for improved post-operative care and highlight the importance of therapeutic interventions like opioid analgesics to mitigate these complications and enhance recovery outcomes in peripheral nerve injury models.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Sciatic nerve injury (SNI) is a common model for studying peripheral nerve damage and regeneration.
📊
Key Findings & Data:For this purpose eighteen female wistar albino rats were divided into three groups: control, sham-operated, and ANI.
💡
Conclusion & Impact:These findings suggest the need for improved post-operative care and highlight the importance of therapeutic interventions like opioid analgesics to mitigate these complications and enhance recovery outcomes in peripheral nerve injury models.
🔬Design:Modeling & Simulation
📍Scope:Comprehensive Sample
⚙️Variables:Complications · Laboratory animal models · Sciatic nerve injury

Abstract

Sciatic nerve injury (SNI) is a common model for studying peripheral nerve damage and regeneration. This study investigates the complications associated with acute nerve injury (ANI) by laceration of sciatic nerve in rats including infection, edema, and cannibalism, and evaluates the effectiveness of therapeutic interventions to modulate the observed complications. For this purpose eighteen female wistar albino rats were divided into three groups: control, sham-operated, and ANI. The ANI model induced with dissection and repair of the right sciatic nerve. Post-surgical care included the administration of diclofenac sodium for pain management. Observations were made for signs of infection, edema, hematoma, and survival rates within 10 days. The ANI group showed significant complications, including a 41.6% incidence of symptoms of pain (paraesthesia, allodynia, hyperalgesia, decreased activity, piloerection, excessive licking, un-groomed appearance) within 3 days, which increased to 60% by day 5. Edema was observed in 8.3% of the ANI rats, and 33.3% developed hematomas. Cannibalism rates also increased, particularly within 10 days post-injury. Survival rates in the ANI group decreased to 16.6% by day 10, indicating severe post-operative complications. The current study highlights the critical complications associated with ANI in rats, particularly the high rates of pain related symptoms (i.e. paresthesia and cannibalism). These findings suggest the need for improved post-operative care and highlight the importance of therapeutic interventions like opioid analgesics to mitigate these complications and enhance recovery outcomes in peripheral nerve injury models.

🔖

Cite This Article

Öztürk, V., Dastan, A. E., Ruşen, Y. A., Barış, E. (2025). Complications and therapeutic approaches in a sciatic nerve injury rat model. Journal of advances in vetbio science and techniques, Volume: 10 Issue: 1. https://doi.org/10.31797/vetbio.1564478

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (22)

  1. Andersson, G., Orädd, G., Sultan, F., & Novikov, L. N. (2018). In vivo diffusion tensor imaging, diffusion kurtosis imaging, and tractography of a sciatic nerve injury model in rat at 9.4T. Scientific Reports, 8(1), 12911. https://doi.org/10.1038/s41598-018-30961-1
  2. Austin, P. J., Wu, A., & Moalem-Taylor, G. (2012). Chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats. Journal of Visualized Experiments: JoVE, 61.3393 https://doi.org/10.3791/3393
  3. Bostan, H., Cabalar, M., Altinay, S., Kalkan, Y., Tumkaya, L., Kanat, A., Balik, S., Erkut, A., Altuner, D., Salihoglu, Z., & Kocer, A. (2018). Sciatic nerve injury following analgesic drug injection in rats: A histopathological examination. Northern Clinics of Istanbul, 5(3), 176–185. https://doi.org/10.14744/nci.2017.28190
  4. Ellis, A., & Bennett, D. L. H. (2013). Neuroinflammation and the generation of neuropathic pain. British Journal of Anaesthesia, 111(1), 26–37. https://doi.org/10.1093/bja/aet128
  5. Feng, X., & Yuan, W. (2015). Dexamethasone enhanced functional recovery after sciatic nerve crush injury in rats. BioMed Research International, 2015(1), 627923. https://doi.org/https://doi.org/10.1155/2015/627923
  6. Guo, N., & Gu, X. (2014). Sciatic nerve neuropathy in cynomolgus monkey macaca fascicularis: altered leg usage and peripheral nerve firing. Journal of Neurology & Neurophysiology, 05(06), 1-6. https://doi.org/10.4172/2155-9562.1000247
  7. Heinzel, J. C., Hercher, D., & Redl, H. (2020). The course of recovery of locomotor function over a 10-week observation period in a rat model of femoral nerve resection and autograft repair. Brain and Behavior, 10(4), e01580. https://doi.org/10.1002/brb3.1580
  8. Huang, W., Yi, S., & Zhao, L. (2023). Genetic features of young and aged animals after peripheral nerve injury: implications for diminished regeneration capacity. Cellular and Molecular Neurobiology, 43(8), 4363–4375. https://doi.org/10.1007/s10571-023-01431-8
  9. Kaya, H., Sabah, D., Keçeci, B., Küçük, L., Erbaş, O., Oltulu, F., Yiğittürk, G., & Taskiran, D. (2021). Comparison of the effects of extracorporeal irradiation and liquid nitrogen on nerve recovery in a rat model. Journal of Investigative Surgery, 34(7), 773–783. https://doi.org/10.1080/08941939.2019.1691686
  10. Kaya, K., & Alizade, A. (2022). Evaluation of diclofenac sodium injection induced neuropathy. Acta Medica Mediterranea, 38(1), 733–739. https://doi.org/10.19193/0393-6384_2022_1_114
  11. Kusama-Eguchi, K., Kawaguchi, K., Yakubo, S., Kitanaka, S., Matsuzaki, K., Takamiya, T., Fukuda, N., Masuko, T., Hirose, D., Makino, M., Ueda, Y., Ikegami, F., & Iijima, H. (2016). Saikokaryukotsuboreito during pregnancy protects rat neonates from maternal cannibalism and death in a neurolathyrism experimental model. Traditional & Kampo Medicine, 3(2), 107–111. https://doi.org/https://doi.org/10.1002/tkm2.1047
  12. Liu, L., Tian, D., Liu, C., Yu, K., & Bai, J. (2019). Metformin enhances functional recovery of peripheral nerve in rats with sciatic nerve crush injury. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 25, 10067–10076. https://doi.org/10.12659/MSM.918277
  13. Pertin, M., Gosselin, R.-D., & Decosterd, I. (2012). The spared nerve injury model of neuropathic pain. Methods in Molecular Biology (Clifton, N.J.), 851, 205–212. https://doi.org/10.1007/978-1-61779-561-9_15
  14. Sonohata, M., Doi, A., Uchihashi, K., Hashimoto, A., Kii, S., Inoue, T., & Mawatari, M. (2023). Short-term collagen nerve wrapping facilitates motor and sensory recovery from nerve degeneration in a sciatic nerve injury rat model. Journal of Pain Research, 16, 1683–1695. https://doi.org/10.2147/JPR.S401126
  15. Sun, H., Yang, T., Li, Q., Zhu, Z., Wang, L., Bai, G., Li, D., Li, Q., & Wang, W. (2012). Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor. Archives of Medical Science: AMS, 8(5), 924–930. https://doi.org/10.5114/aoms.2012.31623
  16. Wang, C.-Z., Chen, Y.-J., Wang, Y.-H., Yeh, M.-L., Huang, M.-H., Ho, M.-L., Liang, J.-I., & Chen, C.-H. (2014). Low-level laser irradiation improves functional recovery and nerve regeneration in sciatic nerve crush rat injury model. PloS One, 9(8), e103348. https://doi.org/10.1371/journal.pone.0103348
  17. Xing, L., Cheng, Q., Zha, G., & Yi, S. (2017). Transcriptional profiling at high temporal resolution reveals robust immune/inflammatory responses during rat sciatic nerve recovery. Mediators of Inflammation, 2017, 3827841. https://doi.org/10.1155/2017/3827841
  18. Yu, C., Wang, X., & Qin, J. (2023). Effect of necrostatin-1 on sciatic nerve crush injury in rat models. Journal of Orthopaedic Surgery and Research, 18(1), 74. https://doi.org/10.1186/s13018-023-03565-3
  19. Zhang, R.-R., Chen, S.-L., Cheng, Z.-C., Shen, Y.-Y., Yi, S., & Xu, H. (2020). Characteristics of cytokines in the sciatic nerve stumps and DRGs after rat sciatic nerve crush injury. Military Medical Research, 7(1), 57. https://doi.org/10.1186/s40779-020-00286-0
  20. Austin, P. J., Wu, A., & Moalem-Taylor, G. (2012). Chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats. Journal of Visualized Experiments: JoVE, 61.3393 https://doi.org/10.3791/3393
  21. Liu, L., Tian, D., Liu, C., Yu, K., & Bai, J. (2019). Metformin enhances functional recovery of peripheral nerve in rats with sciatic nerve crush injury. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research, 25, 10067–10076. https://doi.org/10.12659/MSM.918277
  22. Sun, H., Yang, T., Li, Q., Zhu, Z., Wang, L., Bai, G., Li, D., Li, Q., & Wang, W. (2012). Dexamethasone and vitamin B(12) synergistically promote peripheral nerve regeneration in rats by upregulating the expression of brain-derived neurotrophic factor. Archives of Medical Science: AMS, 8(5), 924–930. https://doi.org/10.5114/aoms.2012.31623