Journals / Journal of advances in vetbio science and techniques / 2026 / Volume: 10 Issue: 1

Investigation of pollen analysis and antimicrobial effects of honey from Posof (Ardahan) district

Pages
—
Publication Date
2025-04-30
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Antimicrobial activity of the honey samples was tested at different concentrations (0.50%, 0.25%, 0.125%) against eight bacterial strains, including Gram-positive bacteria Staphylococcus aureus (ATCC 29213), Staphylococcus aureus (ATCC 25925), and Enterococcus faecalis (ATCC 29219), and Gram-negative bacteria Klebsiella pneumoniae (ATCC 700603), Escherichia coli (O157:H7 RSSK 09007), Escherichia coli (ATCC 25922), Escherichia coli (ATCC 35218), and Pseudomonas aeruginosa (ATCC 27853) using the disc diffusion method.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to determine the pollen composition and antimicrobial activity of honey samples from Posof District, Ardahan Province, located in the Eastern Anatolia region of Türkiye.
📊
Key Findings & Data:A total of 29 honey samples were collected from various villages in Posof.
💡
Conclusion & Impact:Antimicrobial activity of the honey samples was tested at different concentrations (0.50%, 0.25%, 0.125%) against eight bacterial strains, including Gram-positive bacteria Staphylococcus aureus (ATCC 29213), Staphylococcus aureus (ATCC 25925), and Enterococcus faecalis (ATCC 29219), and Gram-negative bacteria Klebsiella pneumoniae (ATCC 700603), Escherichia coli (O157:H7 RSSK 09007), Escherichia coli (ATCC 25922), Escherichia coli (ATCC 35218), and Pseudomonas aeruginosa (ATCC 27853) using the disc diffusion method.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Honey · pollen analysis · antibacterial activity

Abstract

This study aimed to determine the pollen composition and antimicrobial activity of honey samples from Posof District, Ardahan Province, located in the Eastern Anatolia region of Türkiye. A total of 29 honey samples were collected from various villages in Posof. As a result of melissopalynological analysis in these honey samples, 19 different plant families were identified. A total of 18 honey samples were identified as monofloral, while the remaining 11 were classified as multifloral. One sample was dominated by pollen from the Cistaceae family, while the dominant pollen in other monofloral honey samples belonged to the Fabaceae family. The most abundant pollen types in the honey samples were Fabaceae (40.5%), Rosaceae (11.7%), Lamiaceae (9.3%), Boraginaceae (7.5%), and Cistaceae (7.2%). Antimicrobial activity of the honey samples was tested at different concentrations (0.50%, 0.25%, 0.125%) against eight bacterial strains, including Gram-positive bacteria Staphylococcus aureus (ATCC 29213), Staphylococcus aureus (ATCC 25925), and Enterococcus faecalis (ATCC 29219), and Gram-negative bacteria Klebsiella pneumoniae (ATCC 700603), Escherichia coli (O157:H7 RSSK 09007), Escherichia coli (ATCC 25922), Escherichia coli (ATCC 35218), and Pseudomonas aeruginosa (ATCC 27853) using the disc diffusion method.

🔖

Cite This Article

Uluçay, A. G., Akpınar, S. (2025). Investigation of pollen analysis and antimicrobial effects of honey from Posof (Ardahan) district. Journal of advances in vetbio science and techniques, Volume: 10 Issue: 1. https://doi.org/10.31797/vetbio.1543971

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (52)

  1. Acar, H. (2021). Orta asya türklerinde bal ve balın tıbbi amaçlı kullanımı (apiterapi). Mersin Üniversitesi Tıp Fakültesi Lokman Hekim Tıp Tarihi Ve Folklorik Tıp Dergisi, 11(3), 612-624. https://doi.org/10.31020/mutftd.982010
  2. Almasaudi, S. (2021). The antibacterial activities of honey. Saudi Journal of Biological Sciences, 28(4), 2188-2196. https://doi.org/10.1016/j.sjbs.2020.10.017
  3. Alvarez-Suarez, J. M., Tulipani, S., Romandini, S., Bertoli, E., & Battino, M. (2009). Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism, 3(1), 15-23. https://doi.org/10.1007/s12349-009-0051-6
  4. Anand, S., Deighton, M., Livanos, G., Morrison, P. D., Pang, E. C., & Mantri, N. (2019). Antimicrobial activity of Agastache honey and characterization of its bioactive compounds in comparison with important commercial honeys. Frontiers in Microbiology, 10, 263. https://doi.org/10.3389/fmicb.2019.00263
  5. Anklam, E. (1998). A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, 63, 549–562. https://doi.org/10.1016/S0308-8146(98)00057-0
  6. Apan, M. A., Zorba, M., & Kayaboynu, Ü. (2021). Bal arısı ve bal arısı ürünleri. Sinop Üniversitesi Fen Bilimleri Dergisi, 6(2), 202-223. https://doi.org/10.33484/sinopfbd.992345
  7. Barbattini, R., Greatti, M., Iob M., Sabatini A. G., Marcazzan G. L., & Colombo, R. (1991). Osservazioni su Metcalfa pruinosa (Say) e indagine sulle caratteristiche del miele derivato dalla sua melata. Apicoltura, 7, 113-135.
  8. Bayram, N. E., Yüzer, M. O., & Bayram, S. (2019). Melissopalynology analysis, physicochemical properties, multi-element content and antimicrobial activity of honey samples collected from Bayburt, Turkey. Uludağ Arıcılık Dergisi, 19(2), 161-176. https://doi.org/10.31467/uluaricilik.618289
  9. Bhushanam, M., Madhusudhan, S., Bajpai, M., & Sibi, G. (2021). Physicochemical and antibacterial activities of apis honey types derived from Coorg, Karnataka, India. Journal of Applied and Natural Science, 13(2), 729-734. https://doi.org/10.31018/jans.v13i2.2710
  10. Burgut, A., Kutlu, H., & Tufan, M. (2023). Bal arılarında (Apis mellifera L) Spirulina platensis alginin, Varroa destructor parazitine karşı kullanımı ve bal arısı kolonilerinin performansı üzerine etkilerinin araştırılması. Çukurova Tarım ve Gıda Bilimleri Dergisi, 38(1): 14-25. https://doi.org/10.36846/cjafs.2023.95
  11. Cengiz, M. M. (2018). Arıcılık ve organik bal üretimi i̇çin Narman (Erzurum, Türkiye) doğal meralarında ballı bitki potansiyeli. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi 8(2), 358-64. https://doi.org/10.17714/gumusfenbil.371886
  12. Dülgeroğlu, C., & Aksoy, A. (2018). Küresel i̇klim değişikliğinin Origanum minutiflorum Schwarz & P. H. Davis’in coğrafi dağılımına etkisinin maximum entropi algoritması ile tahmini. Erzincan University Journal of Science and Technology, 11(2), 182-190. https://doi.org/10.18185/erzifbed.384196
  13. Erdoğan, N., Pehlivan, S., & Doğan, C. (2008). Pollen analysis of honeys from Hendek-Akyazı and Kocaali Districts of Adapazarı Province (Turkey). Mellifera, 6(10-12), 20-27.
  14. Erdtman, G. (1969). Handbook of Palynology: an introduction to the study of pollen grains and spores, Denmark: Munksgaard Danmark.
  15. Esen, S. (2010). Ardahan ili, Posof, Damal ve Hanak ilçeleri florası (Master's thesis, Artvin Çoruh Üniversitesi). ProQuest Dissertations & Theses Global.
  16. Faegri, K., & Iversen, J. (1974). Texbook of Pollen Analysis, Denmark: Munksgaard Danmark
  17. Genç, F., & Dodoloğlu, A. (2002). Arıcılığın Temel Esasları. Atatürk Üniversitesi Ziraat Fakültesi Ders Yayınları, Ofset tesisi.
  18. Gençay, Ö. Ç., Özenirler, Ç., Bayram, N. E., Zare, G., & Sorkun, K. (2018). Melissopalynological analysis for geographical marking of Kars honey. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 24(1), 53-59. https://doi.org/10.9775/kvfd.2017.18262
  19. Güneş, M. E. (2021). Chestnut honey as a complementary medicine: determination of antibacterial activity, heavy metal residue and health risk assessment. Journal of Advances in Vetbio Science and Techniques, 6(2), 82-89. https://doi.org/10.31797/vetbio.931144
  20. Gürbüz, S., Özenirler, Ç., Mayda, N., Gençay, Ö. Ç., & Özkök, A. (2019). Pollen spectrum of some honey samples produced in Siirt-Turkey. Hacettepe Journal of Biology and Chemistry, 47(3), 295-303. https://doi.org/10.15671/hjbc.532279
  21. Karagözoğlu, Y., & Kıran, T. R. (2022). Bingöl ili ve yöresine ait arı ürünlerinin biyolojik aktiviteleri. Journal of Medical Topics and Updates, 1(3), 170-180. https://doi.org/10.5281/zenodo.7476195
  22. Karaköse, M., Akbulut, S., & Bayramoğlu, M. M. (2018). Invasive alien species in Espiye (Giresun) forest planning unit. Turkish Journal of Forestry, 19(2), 120-129. https://doi.org/10.18182/tjf.349894
  23. Kambur, M. and Kekeçoğlu, M. (2018). Türkiye bal arısı (Apis mellifera l.) alttürlerinde genetik çeşitlilik kaybı. Anadolu Journal of Agricultural Sciences, 33(1), 73-84. https://doi.org/10.7161/omuanajas.337798
  24. Keskin, Ş., Mayda, N., Keskin, M., & Özkök, A. (2020). Investigation of Bilecik honeys in terms of melissopalynology and chemical analyses. Gıda-Journal of Food, 45(2), 275-289. https://doi.org/10.15237/gida.gd19107
  25. Kösoğlu, M., Topal, E., Tunca, R., Yücel, B., & Yıldızdal, İ. (2019). Bal arılarında kışlama öncesi farklı beslemenin koloni gelişimine etkileri. Anadolu Ege Tarımsal Araştırma Enstitüsü Dergisi, 29(2), 85-92. https://doi.org/10.18615/anadolu.660232
  26. Kunat-Budzyńska, M., Rysiak, A., Wiater, A., Grąz, M., Andrejko, M., Budzyński, M., Brys, M. S., Sudziński, M., Tomczyk, M., Gancarz, M., Rusinek, R., & Ptaszyńska, A. (2023). Chemical composition and antimicrobial activity of new honey varietals. International Journal of Environmental Research and Public Health, 20(3), 2458. https://doi.org/10.3390/ijerph20032458
  27. Külekçi, E., & Bulut, Y. (2016). Evaluation of plant that landscape value of carry some cover plants tourısm potential flora in Oltu and Olur District. Journal of the Institute of Science and Technology, 6(1), 95-95.
  28. Kwakman, P. H. S., Te Velde, A. A., de Boer, L., Vandenbroucke-Grauls, C. M., & Zaat, S. A. (2011). Two major medicinal honeys have different mechanisms of bactericidal activity. PloS one, 6(3), e17709. https://doi.org/10.1371/journal.pone.0017709
  29. Lieux, M. H. (1972). A melissopalynological study of 54 Louisiana (USA) honeys. Review of Palaeobotany and Palynology, 13(2), 95-124. https://doi.org/10.1016/0034-6667(72)90039-5
  30. Louveaux, J., Maurizio, A., & Vorwohl, G. (1978). Methods of melissopalynology. Bee world, 59(4), 139-157. https://doi.org/10.1080/0005772X.1978.11097714
  31. Maurizio, A., & Hodges, F. E. D. (1951). Pollen analysis of honey. Bee world, 32(1), 1-5. https://doi.org/10.1080/0005772X.1951.11094660
  32. Mercan, N., Guvensen, A., Celik, A., & Katircioglu, H. (2007). Antimicrobial activity and pollen composition of honey samples collected from different provinces in Turkey. Natural Product Research, 21(3), 187-195. https://doi.org/10.1080/14786410600906277
  33. Mısır, M., İşkil, R., & Kaya, Z. (2023). Arıt bölgesi (Bartın) ballarında polen analizi. Bartın Orman Fakültesi Dergisi, 25(1), 71-95. https://doi.org/10.24011/barofd.1092387
  34. Oliveira, A., Sousa, J. C., Silva, A. C., Melo, L., & Sillankorva, S. (2018). Chestnut honey and bacteriophage application to control Pseudomonas aeruginosa and Escherichia coli biofilms: evaluation in an ex vivo wound model. Frontiers in Microbiology, 9, 1725. https://doi.org/10.3389/fmicb.2018.01725
  35. Onbaşlı, D. (2019). Apiterapi ve i̇nsan sağlığı üzerine etkileri. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16(1), 49-56. https://doi.org/10.32707/ercivet.538001
  36. Özbakır, G. Ö., & Alişiroğlu, D. G. (2019). Nutritional physiology and metabolism of honey bees. Hayvansal Üretim, 60(1), 67-74. https://doi.org/10.29185/hayuretim.523081
  37. Özkök, A., & Bayram, N. E. (2021). Kestane (Castanea sativa) balı örnekleri̇ni̇n botani̇k ori̇ji̇nleri̇ni̇n doğrulanması ve toplam polen sayıları. Uludağ Arıcılık Dergisi, 21(1), 54-65. https://doi.org/10.31467/uluaricilik.899782
  38. Özler, H. (2015). Melissopalynological analysis of honey samples belonging to different districts of Sinop, Turkey. Mellifera, 15(1), 1-11.
  39. Öztürk, F., & Görhan, K. (2021). A research on bee plants of Mersin central districts (Mediterranean, Taurus, Yenişehir, Mezitli). Mas Journal of Applied Sciences, 8(8). https://doi.org/10.52520/masjaps.99
  40. Pranskuniene, Z., Grisiute, R., Pranskunas, A., & Bernatoniene, J. (2022). Ethnopharmacology for skin diseases and cosmetics during the COVİD-19 pandemic in Lithuania. International Journal of Environmental Research and Public Health, 19(7), 4054. https://doi.org/10.3390/ijerph19074054
  41. Savcı, G., İlçim, A., & Karahan, F. (2018). Hatay’da yayılış gösteren bazı Geranium L. (Geraniaceae) türlerinin palinolojisi ve tohum mikromorfolojileri. Commagene Journal of Biology, 2(2), 28-34. https://doi.org/10.31594/commagene.482198
  42. Saxena, S., Gautam, S., & Sharma, A. (2010). Microbial decontamination of honey of Indian origin using gamma radiation and its biochemical and organoleptic properties. Journal of Food Science, 75(1), M19-M27. https://doi.org/10.1111/j.1750-3841.2009.01405.x
  43. Sorkun, K. (2008). Türkiye’nin nektarlı bitkileri, polenleri ve balları. Ankara: Palme yayıncılık.
  44. Sorkun, K., & Doğan, C. (1995). Pollen analysis of Rize-Anzer (Turkish) honey. Apiacta, 3, 75-81
  45. Sorkun, K., Çelemli, Ö. G., Özenirler, Ç., Bayram, N. E. and Güzel, F. (2014). Palynological investigation of honey produced in Ardahan-Turkey. Bee World, 91(3), 80-83. https://doi.org/10.1080/0005772X.2014.11417612
  46. Stavropoulou, E., Ieronymaki, E., Dimitroulia, E., Constantinidis, T. C., Vrioni, G., Tsatsanis, C., & Tsakris, A. (2022). Anti-inflammatory and antibacterial effects and mode of action of greek arbutus, chestnut, and fir honey in mouse models of inflammation and sepsis. Microorganisms, 10(12), 2374. https://doi.org/10.3390/microorganisms10122374
  47. Şenkul, Ç., & Kaya, S. (2017). Türki̇ye endemi̇k bi̇tki̇leri̇ni̇n coğrafi̇ dağılışları. Türk Coğrafya Dergi̇si̇, 69, 109-120. Şık, L., Güvensen, A., Durmuşkahya, C., & Erol, O. (2017). Pollen analysis of honeys from Ardahan/Turkey. Biological Diversity and Conservation, 10(2), 12-19
  48. Şimşek, F. (2023). Some physical and chemical properties of bee bread and its effects on health. Gida/the Journal of Food, 48(4), 807-818. https://doi.org/10.15237/gida.gd23038
  49. Tel, A. Z., Ortaç, İ., & İlçim, A. (2019). A floristic research on Karatepe-Aslantaş national park and some protected natural/cultural areas (Osmaniye, Türkiye). Commagene Journal of Biology, 3(2), 103-109. https://doi.org/10.31594/commagene.641668
  50. Warakomska Z., & Jaroszynska T. (1992). Analysis of the honeydew honey’s Roztocze. Pszczelnicze Zeszyty Naukowe, 36, 149-156.
  51. Yalazi, E., & Zorba, M. (2020). Antioxidant and antimicrobial properties of honeydew honey of Ida mountains. Turkish Journal of Agriculture - Food Science and Technology, 8(3), 587-593. https://doi.org/10.24925/turjaf.v8i3.587-593.3022.
  52. Zubair, R., & Aziz, N. (2015). As smooth as honey - the historical use of honey as topical medication. Jama Dermatology, 151(10), 1102. https://doi.org/10.1001/jamadermatol.2015.1764