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Journals / Journal of advances in vetbio science and techniques / 2026 / Volume: 10 Issue: 2

Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes.

Ebru Afşar(Cappadocia University)0000-0002-7817-855X
Işıl Eranıl(Cappadocia University)0000-0001-9557-8523
Erkan Özkan0000-0002-5251-2302
Erdem Aslan0000-0002-4992-5915
Kadirhan Doğan0000-0002-0249-1435
Mehmet Öz0000-0003-4167-2623
Pages
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Publication Date
2025-08-31
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

Conclusion: Our results suggest that oral application of P. gingivalis-LPS decreased pro-caspase 3, an inactive form of caspase-3 by HT2AR, probably due to increased cleaved caspase-3 levels, and TQ administration increased pro-caspase-3 levels, leading to improved liver morphological structure.

📌 3 Key Takeaways

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Objective & Hypothesis:Background: The present study aimed to assess the effect of Porphyromonas gingivalis-Lipopolysaccharide (P.gingivalis-LPS) on hepatic disorder mediated by 5-hydroxytryptamine receptor 2A (HT2AR) and whether there is any impact of Thymoquinone (TQ) administration.
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Key Findings & Data:Methods: 10 µl P. gingivalis-LPS (1mg/ml) injected 6 times at 48-hour intervals into the palatal gingiva of rats.
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Conclusion & Impact:Conclusion: Our results suggest that oral application of P. gingivalis-LPS decreased pro-caspase 3, an inactive form of caspase-3 by HT2AR, probably due to increased cleaved caspase-3 levels, and TQ administration increased pro-caspase-3 levels, leading to improved liver morphological structure.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:5-Hydroxytryptamine 2A receptors · Porphyromonas gingivalis · Thymoquinone

Abstract

Background: The present study aimed to assess the effect of Porphyromonas gingivalis-Lipopolysaccharide (P.gingivalis-LPS) on hepatic disorder mediated by 5-hydroxytryptamine receptor 2A (HT2AR) and whether there is any impact of Thymoquinone (TQ) administration. Methods: 10 µl P. gingivalis-LPS (1mg/ml) injected 6 times at 48-hour intervals into the palatal gingiva of rats. TQ was given by oral gavage (10 mg/kg per day) for two weeks. HT2AR and pro-caspase-3 levels were measured by the enzyme-linked immunosorbent assay (ELISA). Morphological changes in the liver were identified by hematoxylin and eosin staining. Results: In the P.gingivalis-LPS injected group, the hepatic HT2AR levels increased while pro-caspase-3 levels decreased, disturbing the liver morphological structure. TQ administration increased pro-caspase-3 levels and improved morphological structure despite increased HT2AR levels. Conclusion: Our results suggest that oral application of P. gingivalis-LPS decreased pro-caspase 3, an inactive form of caspase-3 by HT2AR, probably due to increased cleaved caspase-3 levels, and TQ administration increased pro-caspase-3 levels, leading to improved liver morphological structure. Therephore, our study indicated that oral pathogen P. gingivalis might impact the liver HT2AR-mediated, and TQ may have a beneficial effect.

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Cite This Article

Afşar, E., Eranıl, I., Özkan, E., Aslan, E., Doğan, K., Öz, M. (2025). Investigation of the changes in hepatic 5-hydroxytryptamine 2A receptor levels caused by lipopolysaccharide of Porphyromonas gingivalis and the effect of Thymoquinone on these changes.. Journal of advances in vetbio science and techniques, Volume: 10 Issue: 2. https://doi.org/10.31797/vetbio.1655445

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