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Journals / Journal of advances in vetbio science and techniques / 2026 / Issue: 2

Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever

Adem Şahan(HARRAN ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER PR.)0000-0002-4779-0893
Erdem Gülersoy(HARRAN UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF CLINICAL SCIENCES, DEPARTMENT OF INTERNAL MEDICINE)0000-0001-8511-0150
Ender Dinçer(DOKUZ EYLUL UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF PRE-CLINICAL SCIENCES, VIROLOGY)0000-0003-2885-8415
Canberk Balıkçı(HARRAN UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF CLINICAL SCIENCES, DEPARTMENT OF INTERNAL MEDICINE)0000-0001-7473-5163
İsmail Günal(HARRAN UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF CLINICAL SCIENCES, DEPARTMENT OF INTERNAL MEDICINE)0000-0003-3679-4132
Esma Kısmet(HARRAN UNIVERSITY, FACULTY OF VETERINARY MEDICINE, DEPARTMENT OF CLINICAL SCIENCES, DEPARTMENT OF INTERNAL MEDICINE)0000-0002-2906-074X
Pages
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Publication Date
2026-07-13
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Additionally, complications such as jugular vein distension, which has not been widely emphasized in previous studies, and subcutaneous emphysema, which is less common, have been observed and should be considered in clinical evaluation.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Bovine ephemeral fever (BEF), also known as three-day sickness, is a vector-borne viral disease of cattle.
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Key Findings & Data:It causes significant economic losses due to high fever and a sudden decline in milk yield and is characterized by low mortality but high morbidity (80–100%).
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Conclusion & Impact:Additionally, complications such as jugular vein distension, which has not been widely emphasized in previous studies, and subcutaneous emphysema, which is less common, have been observed and should be considered in clinical evaluation.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Bovine ephemeral fever · cattle · clinical findings

Abstract

Bovine ephemeral fever (BEF), also known as three-day sickness, is a vector-borne viral disease of cattle. It causes significant economic losses due to high fever and a sudden decline in milk yield and is characterized by low mortality but high morbidity (80–100%). The aim of this study was to determine the distribution of acute clinical findings in naturally infected Holstein and Simmental cattle with BEF, to evaluate breed-related differences, and to identify subcutaneous emphysema. A total of 32 PCR-confirmed cattle were evaluated. Vital parameters and clinical findings were assessed and recorded within the first 12 hours after the onset of clinical signs. The distribution of clinical findings was as follows (n=32): Appetite was affected in all animals, with complete anorexia observed in 75% of cases; jugular vein distension, recumbency, tremor and salivation (34.4%); lameness (31.2%); mastitis and complete loss of milk production (12.5%); constipation (6.25%); and subcutaneous emphysema (3.1%). No statistically significant difference was found in the distribution of clinical findings between Holstein and Simmental breeds. Subcutaneous emphysema had the lowest incidence among the observed clinical findings. Additionally, jugular vein distension, a clinical finding not commonly described in the literature, was recorded in both breeds during the acute phase of BEF. The combined evaluation of the clinical findings identified in this study may facilitate the diagnosis of BEF, particularly under field conditions, increase clinical suspicion, and support the early initiation of treatment protocols. Additionally, complications such as jugular vein distension, which has not been widely emphasized in previous studies, and subcutaneous emphysema, which is less common, have been observed and should be considered in clinical evaluation.

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Cite This Article

Şahan, A., Gülersoy, E., Dinçer, E., Balıkçı, C., Günal, İ., Kısmet, E. (2026). Distribution of clinical findings and evaluation of infrequent subcutaneous emphysema in naturally infected Simmental and Holstein cattle with bovine ephemeral fever. Journal of advances in vetbio science and techniques, Issue: 2. https://doi.org/10.31797/vetbio.1908558

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References (37)

  1. Abdel-Hamied, E., Elgendy, I. A., Abdel-Samad, A. G., Abd-Elrahman, A. M., & Saeed, H. E. (2026). Integrated evaluation of immune response, inflammatory biomarkers, clinical features and hemato-biochemical changes in bovine ephemeral fever-affected cattle. Irish Veterinary Journal, 79, 18. https://doi.org/10.1186/s13620-026-00332-8
  2. Akakpo, A. J. (2015). Three-day fever. Revue Scientifique et Technique (Office International des Epizooties), 34(2), 533–538, 525–532. https://doi.org/10.20506/rst.34.2.2377
  3. Amin, A. (2016). Histopathological and immunohistochemical studies on bovine ephemeral fever in cattle. Journal of Advanced Veterinary Research, 6(3), 93–100.
  4. Ammar, K. M., Wassel, M. S., & Shafiek, N. G. (2005). Some studies on bovine ephemeral fever in cattle and buffaloes in Egypt. Journal of Veterinary Medical Research, 15(2), 278–283. https://doi.org/10.21608/jvmr.2005.78019
  5. Ashraf, R., Rashid, S., Riaz, M., Bajwa, M. S., Jamil, A., & Khalid, M. T. (2023). Bovine ephemeral fever (BEF). In One Health Triad (Vol. 1, pp. 134–142). Unique Scientific Publishers. https://doi.org/10.47278/book.oht/2023.21
  6. Ayvazoğlu, C., & Ayvazoğlu Demir, P. (2022). Bovine ephemeral fever in Turkey and its economic effect. Van Veterinary Journal, 33(3), 71–75. https://doi.org/10.36483/vanvetj.1141040
  7. Buczinski, S., Francoz, D., Fecteau, G., & Difruscia, R. (2010). Heart disease in cattle with clinical signs of heart failure: 59 cases. Canadian Veterinary Journal, 51(10), 1123–1129.
  8. Çamkerten, G., Çamkerten, I., Ün, H., Balkan, B. M., Ipek, H., Taskin, A., & Sahin, T. (2019). Oxidant-antioxidant status in bovine ephemeral fever. Indian Journal of Animal Research, 53(3), 408–411.
  9. Çatik, S., Cihan, H., Mecitoğlu, Z., Özyiğit, M. Ö., & Şentürk, S. (2015). Ephemeral fever outbreak in a dairy herd in Adana region. Journal of Biological and Environmental Sciences, 9(25), 27-32.
  10. Caymmi, L. G., Mendonça, M. F. F., Leal, P. V., Pimentel, L. A., De Oliveira-Filho, J. C., Peixoto, T. C., Souza, A. C. S. N., De Lucena, R. B., & Riet-Correa, F. (2025). Chronic edema associated with cor pulmonale in cattle. Animals, 15(17), 2501. https://doi.org/10.3390/ani15172501
  11. Collins, G. S., Reitsma, J. B., Altman, D. G., & Moons, K. G. M. (2015). Transparent reporting of a multivariable prediction model for individual prognosis or diagnosis (TRIPOD): The TRIPOD statement. Journal of British Surgery, 102(3), 148-158. https://doi.org/10.1136/bmj.g7594
  12. Constable, P. D., Hinchcliff, K. W., Done, S. H., & Grünberg, W. (2017). Veterinary medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences.
  13. Down, P. M., Green, M. J., & Hudson, C. D. (2013). Rate of transmission: A major determinant of the cost of clinical mastitis. Journal of Dairy Science, 96(10), 6301–6314. https://doi.org/10.3168/jds.2012-6470
  14. Fentener Van Vlissingen, J. M., Borrens, M., Girod, A., Lelovas, P., Morrison, F., & Torres, Y. S. (2015). The reporting of clinical signs in laboratory animals: FELASA working group report. Laboratory Animals, 49(4), 267–283. https://doi.org/10.1177/0023677215584249
  15. Görücü, F., & Demirkan, İ. (2021). The treatment of tracheal rupture and generalized subcutaneous emphysema in a dog. Kocatepe Veterinary Journal, 14(3), 376–381. https://doi.org/10.30607/kvj.953057
  16. Grünberg, W., & Constable, P. D. (2009). Function and dysfunction of the ruminant forestomach. In D. E. Anderson & D. M. Rings (Eds.), Current Veterinary Therapy: Food Animal Practice (pp. 12–19). Elsevier Inc. https://doi.org/10.1016/B978-141603591-6.10006-5
  17. Hala El Daous, H., El Din, N. A., Nafei, E., Abdallah, M., Abd-El Hamed, A. M., Abdel-Wadoud, I. M., & Hafez, E. (2025). Biosecurity measures, bacterial prevalence and economic implications of environmental mastitis and hygienic milking practices on an Egyptian dairy farm. International Journal of Veterinary Science, 14(1), 79–89. https://doi.org/10.47278/journal.ijvs/2024.193
  18. Lavon, Y., Ezra, E., Friedgut, O., & Behar, A. (2023). Economic aspects of bovine ephemeral fever (BEF) outbreaks in dairy cattle herds. Veterinary Sciences, 10(11), 645. https://doi.org/10.3390/vetsci10110645
  19. Lunagariya, P. M., Shah, S. V., Desai, B. D., Pandya, D. P., & Divekar, B. S. (2015). Management of bovine ephemeral fever in crossbred cattle. Intas Polivet, 16(2), 411–413.
  20. Moons, K. G., Altman, D. G., Reitsma, J. B., Ioannidis, J. P., Macaskill, P., Steyerberg, E. W., Vickers, A. J., Ransohoff, D. F., & Collins, G. S. (2015). Transparent reporting of a multivariable prediction model for individual prognosis or diagnosis (TRIPOD): Explanation and elaboration. Annals of Internal Medicine, 162(1), W1–W73. https://doi.org/10.7326/M14-0698
  21. Mosa, A. H. (2025). Case report of bovine ephemeral fever in Babylon Governorate, Iraq: Clinical presentation and treatment. Hosts and Viruses, 12, 129–134. https://doi.org/10.17582/journal.hv/2025/12.129.134
  22. Nadeem, S., Aslam, R., Sajjad-Ur-Rahman., & Khan, M. K. (2024). Risk analysis and seroprevalence of bovine ephemeral fever virus in Punjab, Pakistan. Veterinary Medicine-Czech, 69(3), 67–76. https://doi.org/10.17221/95/2023-VETMED
  23. Özyörük, F., Özgünlük, İ., İnel Turgut, S., Şengül, Y., Yığman, A. A., & Gülyaz, V. (2025). Environmental and breed-related determinants of bovine ephemeral fever outbreaks in southeastern Türkiye. Veterinary Medicine and Science, 11, e70257. https://doi.org/10.1002/vms3.70257
  24. Paksoy, N., Balikçi, C., Merhan, O., Dinçer, E., Şahan, A., & Bozukluhan, K. (2024). Evaluation of acute phase response in cattle with naturally infected bovine ephemeral fever virus. Acta Veterinaria Brno, 93(2), 129–134. https://doi.org/10.2754/avb202493020129
  25. Percie du Sert, N., Hurst, V., Ahluwalia, A., Alam, S., Avey, M. T., Baker, M., Browne, W. J., Clark, A., Cuthill, I. C., Dirnagl, U., Emerson, M., Garner, P., Holgate, S. T., Howells, D. W., Karp, N. A., Lazic, S. E., Lidster, K., MacCallum, C. J., Macleod, M., Pearl, E. J., Petersen, O. H., Rawle, F., Reynolds, P., Rooney, K., Sena, E. S., Silberberg, S. D., Steckler, T., & Würbel, H. (2020). Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0. PLoS Biology, 18(7), e3000411. https://doi.org/10.1371/journal.pbio.3000411
  26. Polat Dinçer, P. F., Şahan, A., & Nayir, M. B. (2024). Effect of circulating calcitriol (1,25(OH)₂D₃) levels on Th1/Th2 immune response in bovine ephemeral fever. Large Animal Review, 30(4), 157–162.
  27. Pyasi, S., Sahu, N. R., Mohanty, U., & Nayak, D. (2025). Bovine ephemeral fever: From underestimated illness to emerging threat - A review of pathogenesis, economic impact, and future control strategies. Research in Veterinary Science, 196, 105904. https://doi.org/10.1016/j.rvsc.2025.105904
  28. Şahin, T., Çamkerten, İ., & Gürgöze, S. Y. (2004). İneklerdeki üç gün hastalığında bazı biyokimyasal parametreler. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 15(1), 71–73.
  29. St George, T. D. (2004). Bovine ephemeral fever. In J. A. W. Coetzer & R. C. Tustin (Eds.), Infectious diseases of livestock (2nd ed., pp. 1183–1193). Oxford University Press.
  30. Theodoridis, A., & Coetzer, J. A. (1979). Subcutaneous and pulmonary emphysema as complications of bovine ephemeral fever. Onderstepoort Journal of Veterinary Research, 46(3), 125–127.
  31. Uren, M. F., St George, T. D., & Zakrzewski, H. (1989). The effect of anti-inflammatory agents on the clinical expression of bovine ephemeral fever. Veterinary Microbiology, 19(2), 99–111. https://doi.org/10.1016/0378-1135(89)90076-x
  32. Walker, P. J., & Klement, E. (2015). Epidemiology and control of bovine ephemeral fever. Veterinary Research, 46, 124. https://doi.org/10.1186/s13567-015-0262-4
  33. Walker, P. J., Blasdell, K. R., & Joubert, A. (2012). Ephemeroviruses: Arthropod-borne rhabdoviruses of ruminants, with large, complex genomes. In R. G. Dietzgen & I. V. Kuzmin (Eds.), Rhabdoviruses: Molecular taxonomy, evolution, genomics, ecology, host-vector interactions, cytopathology and control (pp. 59–88). Caister Academic Press.
  34. Zaghawa, A. A., Housawi, F., Al-Naeem, A., Elsify, A., & Hegazy, Y. M. (2017). Bovine ephemeral fever epidemics in Kingdom Saudi Arabia: Clinical, epidemiological and molecular investigation. Journal of Infection in Developing Countries, 11(11), 854–860. https://doi.org/10.3855/jidc.8201
  35. Zahid, M., Durrani, A. Z., Ijaz, M., Muhammad, K., Usman, M., Husnain, M., & Kamal, N. (2022). Use of physiological and clinical biomarkers as indicators in field study of bovine ephemeral fever. Pakistan Journal of Zoology, 54(1), 255–263.
  36. Zhao, X., & Lacasse, P. (2008). Mammary tissue damage during bovine mastitis: Causes and control. Journal of Animal Science, 86(Suppl. 13), 57–65. https://doi.org/10.2527/jas.2007-0302
  37. Zheng, F., & Qiu, C. (2012). Phylogenetic relationships of the glycoprotein gene of bovine ephemeral fever virus isolated from mainland China, Taiwan, Japan, Turkey, Israel and Australia. Virology Journal, 9, 268. https://doi.org/10.1186/1743-422X-9-268.