AI Summary & Key Takeaways
AI InsightEvaluating CAPE at different doses, in relation to longer storage timesand fertilization rates, will contribute to a better understanding of its potential applications in reproductive biotechnology.
📌 3 Key Takeaways
Abstract
This study was conducted to investigate the effects of caffeic acid phenyl ester (CAPE), one of the biologically active compo-nents of bee propolis, on spermatological parameters during short-term storage (up to 72 hours at +4 °C) of ram semen. Eja-culates from five Merino rams were mixed and divided into four groups: control, 10 μg/ml, 50 μg/ml, and 100 μg/ml CAPEtreatment groups. Sperm samples were evaluated for motility, abnormal spermatozoon rate, and plasma membrane integrity(HOS test) at 0, 24, 48, and 72 hours. The results revealed that CAPE contributed to the preservation of semen quality, especi-ally at the 100 μg/ml dose. According to the study findings, while no significant differences were found between the groups interms of motility and abnormal spermatozoon rates at 0, 24, and 48 hours, increases in plasma membrane integrity (HOS test),particularly in the 100 μg/ml group, were found to be statistically significant. At 72 hours, the significant increases in motilityvalues and decreases in abnormal spermatozoon rates in the 10 and 100 μg/ml groups indicate that CAPE supports sperm cellmembrane stabilization during short-term storage. The lack of any studies using CAPE for short-term storage of ram semenmakes this research a valuable contribution to the field. Evaluating CAPE at different doses, in relation to longer storage timesand fertilization rates, will contribute to a better understanding of its potential applications in reproductive biotechnology.