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Journals / International Journal of Secondary Metabolite / 2026 / Volume: 11 Issue: 3

Cytotoxic effect of Eleutherococcus trifoliatus (L.) S.Y. Hu stem bark extracts on Gastric, Lung, and Hepatocellular cancer cells

Nguyen Quan(University of Science, Vietnam National University Ho Chi Minh City)0000-0002-6436-4693
Bui Thi Kim Ly(Thu Dau Mot University, Vietnam)0000-0002-8433-7035
Hoang Thanh Chi(Thu Dau Mot University)0000-0002-6638-1235
Pages
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Publication Date
2024-08-29
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The chloroform fraction had the most potential to be further exploited in the direction of inhibiting cancer cells.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Eleutherococcus trifoliatus is a popular medicinal species used in Asian folk medicine with many uses in treating human diseases.
📊
Key Findings & Data:The anticancer effect of the total extract was investigated and indicated the dramatical ability to inhibit the cell growth of liver cancer HCC-J5 cells (IC50 = 19.35 ± 4.89 µg/mL), lung cancer cells A549 (IC50 = 5.34 ± 1.62 µg/mL) and gastric cancer cells AGS (IC50 = 0.22 ± 0.20 µg/mL); the selectivity in effects was also observed.
💡
Conclusion & Impact:The chloroform fraction had the most potential to be further exploited in the direction of inhibiting cancer cells.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Eleutherococcus trifoliatus · Anticancer · HCC-J5

Abstract

Eleutherococcus trifoliatus is a popular medicinal species used in Asian folk medicine with many uses in treating human diseases. Scientific research on this plant has been limited, but some scientific publications have described this herb's anti-inflammatory, antibacterial, antioxidant, and anti-cancer activities. Although it is a frequently used part of traditional medicine, current scientific evidence on the biological activities of E. trifoliatus stem bark is still lacking. By performing chemical reactions, free radical scavenging experiments, toxicity experiments on brine shrimp, and cytotoxicity tests using MTT dye, the study has shown the diversity in the metabolic composition of E. trifoliatus stem bark as well as the antioxidant capacity and safety of the total extract. The anticancer effect of the total extract was investigated and indicated the dramatical ability to inhibit the cell growth of liver cancer HCC-J5 cells (IC50 = 19.35 ± 4.89 µg/mL), lung cancer cells A549 (IC50 = 5.34 ± 1.62 µg/mL) and gastric cancer cells AGS (IC50 = 0.22 ± 0.20 µg/mL); the selectivity in effects was also observed. The chloroform fraction had the most potential to be further exploited in the direction of inhibiting cancer cells.

Özet

Eleutherococcus trifoliatus is a popular medicinal species used in Asian folk medicine with many uses in treating human diseases. Scientific research on this plant has been limited, but some scientific publications have described this herb's anti-inflammatory, antibacterial, antioxidant, and anti-cancer activities. Although it is a frequently used part of traditional medicine, current scientific evidence on the biological activities of E. trifoliatus stem bark is still lacking. By performing chemical reactions, free radical scavenging experiments, toxicity experiments on brine shrimp, and cytotoxicity tests using MTT dye, the study has shown the diversity in the metabolic composition of E. trifoliatus stem bark as well as the antioxidant capacity and safety of the total extract. The anticancer effect of the total extract was investigated and indicated the dramatical ability to inhibit the cell growth of liver cancer HCC-J5 cells (IC50 = 19.35 ± 4.89 µg/mL), lung cancer cells A549 (IC50 = 5.34 ± 1.62 µg/mL) and gastric cancer cells AGS (IC50 = 0.22 ± 0.20 µg/mL); the selectivity in effects was also observed. The chloroform fraction had the most potential to be further exploited in the direction of inhibiting cancer cells.

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Cite This Article

Quan, N., Ly, B. T. K., Chi, H. T. (2024). Cytotoxic effect of Eleutherococcus trifoliatus (L.) S.Y. Hu stem bark extracts on Gastric, Lung, and Hepatocellular cancer cells. International Journal of Secondary Metabolite, Volume: 11 Issue: 3. https://doi.org/10.21448/ijsm.1415631

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References (48)

  1. Ayoola, G., Coker, H., Adesegun, S., Adepoju-Bello, A., Obaweya, K., Ezennia, E., & Atangbayila, T. (2008). Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria. Tropical Journal of Pharmaceutical Research, 7(3), 7. https://doi.org/10.4314/tjpr.v7i3.14686
  2. Baumli, J., Antal, N., Casoni, D., & Cimpoiu, C. (2023). Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle. Plants, 12(18), 3233. https://www.mdpi.com/2223-7747/12/18/3233
  3. Blois, M.S. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature, 181(4617), 1199-1200. https://doi.org/10.1038/1811199a0
  4. Ciulei, I. (1993). Plante medicinale, fitochimie si fitoterapie. (Vol. II). Editura medicală.
  5. Chen, Z., Cheng, S., Lin, H., Wu, W., Liang, L., Chen, X.,., Zhang, K. (2021). Antibacterial, anti-inflammatory, analgesic, and hemostatic activities of Acanthopanax trifoliatus (L.) merr. Food Science & Nutrition, 9(4), 2191-2202. https://doi.org/10.1002/fsn3.2190
  6. Chien, T.-M., Hsieh, P.-C., Huang, S.-S., Deng, J.-S., Ho, Y.-L., Chang, Y.-S., & Huang, G.-J. (2015). Acanthopanax trifoliatus inhibits lipopolysaccharide-induced inflammatory response in vitro and in vivo. The Kaohsiung Journal of Medical Sciences, 31(10), 499-509. https://doi.org/10.1016/j.kjms.2015.07.007
  7. Dedvisitsakul, P., & Watla-Iad, K. (2022). Antioxidant activity and antidiabetic activities of Northern Thai indigenous edible plant extracts and their phytochemical constituents. Heliyon, 8(9), e10740. https://doi.org/10.1016/j.heliyon.2022.e10740
  8. Edeoga, H.O., Okwu, D.E. & Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. African Journal of Biotechnology, 4(7), 685-688.
  9. Einafshar, E., Mobasheri, L., Hasanpour, M., Rashidi, R., & Ghorbani, A. (2024). Pro-apoptotic effect of chloroform fraction of Moraea sisyrinchium bulb against glioblastoma cells. Biomedicine & Pharmacotherapy, 170, 115931. https://doi.org/10.1016/j.biopha.2023.115931
  10. Ferraz, C.A., Pastorinho, M.R., Palmeira-de-Oliveira, A., & Sousa, A.C.A. (2022). Ecotoxicity of plant extracts and essential oils: A review. Environmental Pollution, 292, 118319. https://doi.org/10.1016/j.envpol.2021.118319
  11. Ganogpichayagrai, A., & Suksaard, C. (2020). Proximate composition, vitamin and mineral composition, antioxidant capacity, and anticancer activity of Acanthopanax trifoliatus. Journal of Advanced Pharmaceutical Technology & Research, 11(4), 179-183. https://doi.org/10.4103/japtr.JAPTR_61_20
  12. Harwig, J., & Scott, P.M. (1971). Brine shrimp (Artemia salina L.) larvae as a screening system for fungal toxins. Applied Microbiology, 21(6), 1011 1016. https://doi.org/10.1128/am.21.6.1011-1016.1971
  13. Huyen, P.T., Tap, N.V., Son, L.T., Phuong, N.D., Long, C.H., My, D.V.,., Thin, N N. (2006). Nghiên cứu khả năng nhân giống và bảo tồn ngũ gia bì hương và ngũ gia bì gai ở Việt Nam [Research on the possibility of propagating and preserving Eleutherococcus nodiflorus and Eleutherococcus trifoliatus in Vietnam]. Tạp chí Dược liệu, 11(3), 108-113.
  14. Indrayanto, G., Putra, G.S., & Suhud, F. (2021). Validation of in-vitro bioassay methods: Application in herbal drug research. Profiles of Drug Substances, Excipients and Related Methodology, 46, 273-307. https://doi.org/10.1016/bs.podrm.2020.07.005
  15. Jha, D., Panda, L., Pandian, L., Ramaiah, S., & Anbarasu, A. (2012). Detection and Confirmation of Alkaloids in Leaves of Justicia adhatoda and Bioinformatics Approach to Elicit Its Anti-tuberculosis Activity. Applied Biochemistry and Biotechnology, 168. https://doi.org/10.1007/s12010-012-9834-1
  16. Kancheva, V.D., & Kasaikina, O.T. (2013). Bio-antioxidants - a chemical base of their antioxidant activity and beneficial effect on human health. Current Medicinal Chemistry, 20(37), 4784-4805. https://doi.org/10.2174/09298673113209990161
  17. Li, D., Li, Z., Yang, J., Hao, Z., Yang, J., & Lin, C. (2021). Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury. Science and Technology of Food Industry, 42(18), 380-386. https://doi.org/10.13386/j.issn1002-0306.2021010238
  18. Li, D.L., Zheng, X., Chen, Y.C., Jiang, S., Zhang, Y., Zhang, W.M.,. Zhang, K. (2016). Terpenoid composition and the anticancer activity of Acanthopanax trifoliatus. Archives of Pharmacal Research, 39(1), 51-58. https://doi.org/10.1007/s12272-015-0655-y
  19. Li, X., Wu, Q., Xie, Y., Ding, Y., Du, W.W., Sdiri, M., & Yang, B.B. (2015). Ergosterol purified from medicinal mushroom Amauroderma rude inhibits cancer growth in vitro and in vivo by up-regulating multiple tumor suppressors. Oncotarget, 6(19), 17832-17846. https://doi.org/10.18632/oncotarget.4026
  20. Lin, Y., Pan, J., Liu, Y., Yang, H., Wu, G., & Pan, Y. (2023). Acanthopanax trifoliatus (L.) Merr polysaccharides ameliorates hyperglycemia by regulating hepatic glycogen metabolism in type 2 diabetic mice. Frontiers in Nutrition, 10, 1 13. https://doi.org/10.3389/fnut.2023.1111287 Lobo, V., Patil, A., Phatak, A., & Chandra, N. (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4(8), 118 126. https://doi.org/10.4103/0973-7847.70902
  21. Loi, D.T. (2004). Những cây thuốc và vị thuốc Việt Nam [Vietnamese medicinal plants and herbs] (D.T. Loi, Ed. Vol. 1). Medical publishing house.
  22. Ly, B., Quan, N., Dao, L., Nguyen, H., Lam, M., & Hoang, C. (2019). Evaluation of Antimicrobial, Antioxidant and Cytotoxic Activities of Dialium cochinchinensis Seed Extract. Indian Journal of Pharmaceutical Sciences, 81(5), 975 980. https://doi.org/10.36468/pharmaceutical-sciences.594
  23. MacWillliam, I.C., & Wenn, R.V. (1972). Interpretation of colour tests for polyphenols and melanoidins. Brewing Industry Research Foundation, Nutfield, Surrey, 78, 309.
  24. Muhammed, A., Nakalembe, I., Olila, D., Afodun, A., & Mbugua, R. (2021). Anticancer and Apoptotic Potentials of Gliricidia sepium Leaf Extract on Breast HCC1395, Prostate DU145 and Colorectal CT26 Cell Lines. Journal of Cancer and Tumor International, 11(4), 89-100. https://doi.org/10.9734/jcti/2021/v11i430163
  25. Mujeeb, F., Bajpai, P., & Pathak, N. (2014). Phytochemical evaluation, antimicrobial activity, and determination of bioactive components from leaves of Aegle marmelos. BioMed Research International, 2014, 497606. https://doi.org/10.1155/2014/497606
  26. Muselli, A., Hoi, T.M., Cu, L.D., Moi, L.D., Bessière, J.-M., Bighelli, A., & Casanova, J. (1999). Composition of the essential oil of Acanthopanax trifoliatus (L.) Merr. (Araliacaea) from Vietnam. Flavour and Fragrance Journal, 14(1), 41 44. https://doi.org/10.1002/(SICI)1099-1026(199901/02)14:1
  27. Nath, M.C., Chakravorty, M.K., & Chowdhury, S.R. (1946). Liebermann-Burchard Reaction for Steroids. Nature, 157(3978), 103-104. https://doi.org/https://doi.org/10.1038/157103b0
  28. Nogueira, F., & Rosário, V.E.d. (2010). Methods for assessment of antimalarial activity in the different phases of the Plasmodium life cycle. Revista Pan-Amazônica de Saúde, 1, 109-124.
  29. Peña-Morán, O.A., Villarreal, M.L., Álvarez-Berber, L., Meneses-Acosta, A., & Rodríguez-López, V. (2016). Cytotoxicity, Post-Treatment Recovery, and Selectivity Analysis of Naturally Occurring Podophyllotoxins from Bursera fagaroides var. fagaroides on Breast Cancer Cell Lines. Molecules, 21(8). https://doi.org/10.3390/molecules21081013
  30. Phuong, N.T., Lee, K.A., Jeong, S.J., Fu, C.X., Choi, J.K., Kim, Y.H., & Kang, J.S. (2006). Capillary electrophoretic method for the determination of diterpenoid isomers in Acanthopanax species. Journal of Pharmaceutical and Biomedical Analysis, 40(1), 56-61. https://doi.org/10.1016/j.jpba.2005.05.023
  31. Rieser, M.J., Gu, Z.M., Fang, X.P., Zeng, L., Wood, K.V., & McLaughlin, J.L. (1996). Five novel mono-tetrahydrofuran ring acetogenins from the seeds of Annona muricata. Journal of Natural Products, 59(2), 100-108. https://doi.org/10.1021/np960037q
  32. Santos Filipe, M., Isca, V.M.S., Ntungwe, N.E., Princiotto, S., Díaz-Lanza, A.M., & Rijo, P. (2022). Lethality Bioassay using Artemia salina L. Journal of Visualized Experiments: JoVE, 2022 Oct 11 (188). https://doi.org/10.3791/64472
  33. Sithisarn, P., & Jarikasem, S. (2009). Antioxidant activity of Acanthopanax trifoliatus. Medical Principles and Practice, 18(5), 393-398. https://doi.org/10.1159/000226294
  34. Thi, T., Quan, N., Thanh Chi, H., & Ly, B. (2023). An Investigation into the Phytochemical Content, Antibacterial Effect, and Antioxidant Capacity of the Ethanol Extract of Salacca wallichiana Mart. Peels. European Journal of Biology, 82(2), 179 185. https://doi.org/10.26650/EurJBiol.2023.1316545
  35. Thu, N.T.B., & Tuan, D.T. (2013). Anti-inflammatory property of Acanthopanax trifoliatus by suppressing the activity of NF- κB on the macrophagocytes Raw 264.7. Tạp chí Dược học, 53(6), 64-67.
  36. Wang, H.-C., Tseng, Y.-H., Wu, H.-R., Chu, F.-H., Kuo, Y.-H., & Wang, S.-Y. (2014). Anti-proliferation Effect on Human Breast Cancer Cells via Inhibition of pRb Phosphorylation by Taiwanin E Isolated from Eleutherococcus trifoliatus. Natural Product Communications, 9(9), 1934578X1400900921. https://doi.org/s10.1177/1934578x1400900921
  37. Wang, H.Q., Li, D.L., Lu, Y.J., Cui, X.X., Zhou, X.F., Lin, W.P.,. Zheng, X. (2014). Anticancer activity of Acanthopanax trifoliatus (L) Merr extracts is associated with inhibition of NF-kB activity and decreased Erk1/2 and Akt phosphorylation. Asian Pacific Journal of Cancer Prevention, 15(21), 9341 9346. https://doi.org/10.7314/apjcp.2014.15.21.9341
  38. Weerapreeyakul, N., Nonpunya, A., Barusrux, S., Thitimetharoch, T., & Sripanidkulchai, B. (2012). Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chinese Medicine, 7(1), 15. https://doi.org/10.1186/1749-8546-7-15
  39. Wiart, C. (2007). Ethnopharmacology of medicinal plants: Asia and the Pacific. Springer Science & Business Media.
  40. Zhang, X., Bai, Y., Wang, Y., Wang, C., Fu, J., Gao, L.,., Deng, Y. (2020). Anticancer Properties of Different Solvent Extracts of Cucumis melo L. Seeds and Whole Fruit and Their Metabolite Profiling Using HPLC and GC-MS. BioMed Research International, 2020, 5282949. https://doi.org/10.1155/2020/5282949
  41. Ciulei, I. (1993). Plante medicinale, fitochimie si fitoterapie. (Vol. II). Editura medicală.
  42. Chen, Z., Cheng, S., Lin, H., Wu, W., Liang, L., Chen, X.,., Zhang, K. (2021). Antibacterial, anti-inflammatory, analgesic, and hemostatic activities of Acanthopanax trifoliatus (L.) merr. Food Science & Nutrition, 9(4), 2191-2202. https://doi.org/10.1002/fsn3.2190
  43. Edeoga, H.O., Okwu, D.E. & Mbaebie, B.O. (2005). Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. African Journal of Biotechnology, 4(7), 685-688.
  44. Huyen, P.T., Tap, N.V., Son, L.T., Phuong, N.D., Long, C.H., My, D.V.,., Thin, N N. (2006). Nghiên cứu khả năng nhân giống và bảo tồn ngũ gia bì hương và ngũ gia bì gai ở Việt Nam [Research on the possibility of propagating and preserving Eleutherococcus nodiflorus and Eleutherococcus trifoliatus in Vietnam]. Tạp chí Dược liệu, 11(3), 108-113.
  45. Li, D.L., Zheng, X., Chen, Y.C., Jiang, S., Zhang, Y., Zhang, W.M.,. Zhang, K. (2016). Terpenoid composition and the anticancer activity of Acanthopanax trifoliatus. Archives of Pharmacal Research, 39(1), 51-58. https://doi.org/10.1007/s12272-015-0655-y
  46. Santos Filipe, M., Isca, V.M.S., Ntungwe, N.E., Princiotto, S., Díaz-Lanza, A.M., & Rijo, P. (2022). Lethality Bioassay using Artemia salina L. Journal of Visualized Experiments: JoVE, 2022 Oct 11 (188). https://doi.org/10.3791/64472
  47. Wang, H.Q., Li, D.L., Lu, Y.J., Cui, X.X., Zhou, X.F., Lin, W.P.,. Zheng, X. (2014). Anticancer activity of Acanthopanax trifoliatus (L) Merr extracts is associated with inhibition of NF-kB activity and decreased Erk1/2 and Akt phosphorylation. Asian Pacific Journal of Cancer Prevention, 15(21), 9341 9346. https://doi.org/10.7314/apjcp.2014.15.21.9341
  48. Zhang, X., Bai, Y., Wang, Y., Wang, C., Fu, J., Gao, L.,., Deng, Y. (2020). Anticancer Properties of Different Solvent Extracts of Cucumis melo L. Seeds and Whole Fruit and Their Metabolite Profiling Using HPLC and GC-MS. BioMed Research International, 2020, 5282949. https://doi.org/10.1155/2020/5282949