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Journals / International Journal of Secondary Metabolite / 2026 / Volume: 13 Issue: 3

Essential oils: A review of ex vivo studies on aortic smooth muscle contractility

Sadettin Demirel(TIP FAKÜLTESİ)0000-0002-3629-5344
İpek Nazlı Sinağ(Bursa Uludag University)0009-0008-8810-9276
Pages
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Publication Date
2026-09-02
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This paper provides a comprehensive review of the essential oils with vasoactive effects on the aorta function and the underlying signaling pathways involved.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Essential oils, known as volatile oils, are valuable odoriferous substances from plants.
📊
Key Findings & Data:The popularity of essential oils has significantly increased due to numerous therapeutic properties, including antihypertensive, antidiabetic, antidyslipidemic, and antiplatelet activities.
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Conclusion & Impact:This paper provides a comprehensive review of the essential oils with vasoactive effects on the aorta function and the underlying signaling pathways involved.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Essential oil · Aorta · Vasoactive effect

Abstract

Essential oils, known as volatile oils, are valuable odoriferous substances from plants. The popularity of essential oils has significantly increased due to numerous therapeutic properties, including antihypertensive, antidiabetic, antidyslipidemic, and antiplatelet activities. A total of 42 essential oils were detected involved in the aorta contraction-relaxation mechanisms ex vivo. The majority of these essential oils exhibit smooth muscle relaxant effects via multiple signaling pathways. Therefore, the plant-derived essential oils may be considered a potential agent for the treatment of cardiovascular diseases. This paper provides a comprehensive review of the essential oils with vasoactive effects on the aorta function and the underlying signaling pathways involved.

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Cite This Article

Demirel, S., Sinağ, İ. N. (2026). Essential oils: A review of ex vivo studies on aortic smooth muscle contractility. International Journal of Secondary Metabolite, Volume: 13 Issue: 3. https://doi.org/10.21448/ijsm.1773457

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References (51)

  1. Al-habib, O.A.M., Kheder, D.A., Vidari, G., & Gilardoni, G. (2013). Relaxant effects of essential oils of Eucalyptus camaldulensis on aortic rings in male albino rats. Science Journal of University of Zakho, 1A(1), 139-146.
  2. Alkharfy, K.M., Ahmad, A., Almuaijel, S., Bin Hashim, A., Raish, M., Jan, B.L., Rehman, N.U., Anwar, F., Rehman, M.T., & Alajmi, M.F. (2024). The vascular effects of peppermint (Mentha longifolia. L) on aorta in a mouse model: An ex-vivo and computational study. Journal of Biomolecular Structure & Dynamics, 1-16. https://doi.org/10.1080/07391102.2024.2439616
  3. Alves-Silva, J.M., Zuzarte, M., Girão, H., & Salgueiro, L. (2021). The role of essential oils and their main compounds in the management of cardiovascular disease risk factors. Molecules, 26(12), 3506. https://doi.org/10.3390/molecules26123506
  4. Aqel, M.B. (1992). A vascular smooth muscle relaxant effect of Rosmarinus officinalis. International Journal of Pharmacognosy, 30(4), 281-288. https://doi.org/10.3109/13880209209054014
  5. Aydin, S., Demir, T., Oztürk, Y., & Başer, K.H. (1999). Analgesic activity of Nepeta italica L. Phytotherapy Research, 13(1), 20 23. https://doi.org/10.1002/(SICI)1099 1573(199902)13:1 3.0.CO;2-J
  6. Baccelli, C., Martinsen, A., Morel, N., & Quetin-Leclercq, J. (2010). Vasorelaxant activity of essential oils from Croton zambesicus and some of their constituents. Planta Medica, 76(14), 1506-1511. https://doi.org/10.1055/s-0030-1249820
  7. Ballabeni, V., Tognolini, M., Bertoni, S., Bruni, R., Guerrini, A., Rueda, G.M., & Barocelli, E. (2007). Antiplatelet and antithrombotic activities of essential oil from wild Ocotea quixos (Lam.) Kosterm. (Lauraceae) calices from Amazonian Ecuador. Pharmacological Research, 55(1), 23-30. https://doi.org/10.1016/j.phrs.2006.09.009
  8. Boholy, R., Ruphin, F.P., Ngbolua, K.N., Barthelemy, F., Amélie, R., Pierre, R., Claude, M., Mudogo, V., Mpiana, P.T., Samuel, R., & Miadana, R.R. (2014). Evaluation of the vasorelaxant effect induced by the essential oil extracted from the root bark of Hazumalania voyronii (Jum.) Capuron (Hernandiaceae) in wistar rat aorta ring. Journal of Advancement in Medical and Life Sciences, 2(1). https://doi.org/10.15297/JALS.V2I1.04
  9. Chen, J.F., Peng, C., Ni, H., Li, B.Y., Ye, J.W., Liu, F., & Xiong, L. (2025). Endothelium-independent vasorelaxant effects of Curcuma phaeocaulis essential oil and its representative compound isocurcumenol. Journal of Ethnopharmacology, 341, 119366. https://doi.org/10.1016/j.jep.2025.119366
  10. Cherkaoui-Tangi, K., Israili, Z.H., & Lyoussi, B. (2016). Vasorelaxant effect of essential oil isolated from Nigella sativa L. seeds in rat aorta: Proposed mechanism. Pakistan Journal of Pharmaceutical Sciences, 29(1), 1-8.
  11. Correia, A.C., Ferreira, T.F., Martins, I.R., Macêdo, C.L., Monteiro, F.deS., Costa, V.C., Tavares, J.F., Silva, M.S., Paredes-Gamero, E.J., Buri, M.V., Rigoni, V.L., Nouailhetas, V.L., & Da Silva, B.A. (2015). Essential oil from the leaves of Xylopia langsdorfiana (Annonaceae) as a possible spasmolytic agent. Natural Product Research, 29(10), 980 984. https://doi.org/10.1080/14786419.2014.964706
  12. da Silva, R.E.R., de Morais, L.P., Silva, A.A., Bastos, C.M.S., Pereira-Gonçalves, Á., Kerntopf, M.R., Menezes, I.R.A., Leal-Cardoso, J.H., & Barbosa, R. (2018). Vasorelaxant effect of the Lippia alba essential oil and its major constituent, citral, on the contractility of isolated rat aorta. Biomedicine & Pharmacotherapy, 108, 792-798. https://doi.org/10.1016/j.biopha.2018.09.073
  13. Dajic Stevanovic, Z., Sieniawska, E., Glowniak, K., Obradovic, N., & Pajic-Lijakovic, I. (2020). Natural macromolecules as carriers for essential oils: From extraction to biomedical application. Frontiers in Bioengineering and Biotechnology, 8, 563. https://doi.org/10.3389/fbioe.2020.00563
  14. de França-Neto, A., Cardoso-Teixeira, A.C., Medeiros, T.C., Quinto-Farias, M.doS., Sampaio, C.M., Coelho-de-Souza, A.N., Lahlou, S., & Leal-Cardoso, J.H. (2012). Essential oil of Croton argyrophylloides: Toxicological aspects and vasorelaxant activity in rats. Natural Product Communications, 7(10), 1397-1400.
  15. de Siqueira, R.J., Magalhães, P.J., Leal-Cardoso, J.H., Duarte, G.P., & Lahlou, S. (2006). Cardiovascular effects of the essential oil of Croton zehntneri leaves and its main constituents, anethole and estragole, in normotensive conscious rats. Life Sciences, 78(20), 2365-2372. https://doi.org/10.1016/j.lfs.2005.09.042
  16. de Siqueira, R.J., Rodrigues, K.M., da Silva, M.T., Correia Junior, C.A., Duarte, G.P., Magalhães, P.J., dos Santos, A.A., Maia, J.G., da Cunha, P.J., & Lahlou, S. (2014). Linalool-rich rosewood oil induces vago-vagal bradycardic and depressor reflex in rats. Phytotherapy Research, 28(1), 42-48. https://doi.org/10.1002/ptr.4953
  17. de Sousa, D.P., Damasceno, R.O.S., Amorati, R., Elshabrawy, H.A., de Castro, R.D., Bezerra, D.P., Nunes, V.R.V., Gomes, R.C., & Lima, T.C. (2023). Essential oils: Chemistry and pharmacological activities. Biomolecules, 13(7), 1144. https://doi.org/10.3390/biom13071144
  18. Demirel, S. (2022). Rosa damascena Miller essential oil relaxes rat thoracic aorta through the NO-cGMP dependent pathway. Prostaglandins & Other Lipid Mediators, 162, 106661. https://doi.org/10.1016/j.prostaglandins.2022.106661
  19. Dib, I., Fauconnier, M.L., Sindic, M., Belmekki, F., Assaidi, A., Berrabah, M., Mekhfi, H., Aziz, M., Legssyer, A., Bnouham, M., & Ziyyat, A. (2017). Chemical composition, vasorelaxant, antioxidant and antiplatelet effects of essential oil of Artemisia campestris L. from Oriental Morocco. BMC Complementary and Alternative Medicine, 17(1), 82. https://doi.org/10.1186/s12906-017-1598-2
  20. Esmaeili, H., Sharifi, M., Esmailidehaj, M., Rezvani, M.E., & Hafizibarjin, Z. (2017). Vasodilatory effect of asafoetida essential oil on rat aorta rings: The role of nitric oxide, prostacyclin, and calcium channels. Phytomedicine, 36, 88-94. https://doi.org/10.1016/j.phymed.2017.10.002
  21. Hamid, A.A., Aiyelaagbe, O.O., & Usman, L.A. (2011). Essential oils: Its medicinal and pharmacological uses. International Journal of Current Research, 3(2), 086-098.
  22. Itthipanichpong, C., Ruangrungsi, N., & Pattanaautsahakit, C. (2002). Chemical compositions and pharmacological effects of essential oil from the fruit of Zanthoxylum limonella. Journal of the Medical Association of Thailand, 85 Suppl 1, S344-S354.
  23. Ivanova, S., Gvozdeva, Y., Staynova, R., Grekova-Kafalova, D., Nalbantova, V., Benbassat, N., Koleva, N., & Ivanov, K. (2025). Essential oils – a review of the natural evolution of applications and some future perspectives. Pharmacia, 72, 1-12. https://doi.org/10.3897/pharmacia.72.e140059
  24. Kang, P., Ryu, K.H., Lee, J.M., Kim, H.K., & Seol, G.H. (2016). Endothelium- and smooth muscle-dependent vasodilator effects of Citrus aurantium L. var. amara: Focus on Ca(2+) modulation. Biomedicine & Pharmacotherapy, 82, 467-471. https://doi.org/10.1016/j.biopha.2016.05.030
  25. Kang, P., Suh, S.H., Min, S.S., & Seol, G.H. (2013). The essential oil of Citrus bergamia Risso induces vasorelaxation of the mouse aorta by activating K(+) channels and inhibiting Ca(2+) influx. The Journal of Pharmacy and Pharmacology, 65(5), 745-749. https://doi.org/10.1111/jphp.12031
  26. Lahlou, S., Magalhães, P.J., Carneiro-Leão, R.F., & Leal-Cardoso, J.H. (2002). Involvement of nitric oxide in the mediation of the hypotensive action of the essential oil of Mentha x villosa in normotensive conscious rats. Planta Medica, 68(8), 694-699. https://doi.org/10.1055/s-2002-33797
  27. Lahlou, S., Magalhães, P.J., de Siqueira, R.J., Figueiredo, A.F., Interaminense, L.F., Maia, J.G., & Sousa, P.J. (2005). Cardiovascular effects of the essential oil of Aniba canelilla bark in normotensive rats. Journal of Cardiovascular Pharmacology, 46(4), 412 421. https://doi.org/10.1097/01.fjc.0000175876.25296.f4
  28. Magalhães, P.J., Lahlou, S., Jucá, D.M., Coelho-de-Souza, L.N., da Frota, P.T., da Costa, A.M., & Leal-Cardoso, J.H. (2008). Vasorelaxation induced by the essential oil of Croton nepetaefolius and its constituents in rat aorta are partially mediated by the endothelium. Fundamental & Clinical Pharmacology, 22(2), 169-177. https://doi.org/10.1111/j.1472-8206.2008.00571.x
  29. Maiwulanjiang, M., Bi, C.W., Lee, P.S., Xin, G., Miernisha, A., Lau, K.M., Xiong, A., Li, N., Dong, T.T., Aisa, H.A., & Tsim, K.W. (2015). The volatile oil of Nardostachyos Radix et Rhizoma induces endothelial nitric oxide synthase activity in HUVEC cells. PloS One, 10(2), e0116761. https://doi.org/10.1371/journal.pone.0116761
  30. Martišienė, I., Zigmantaitė, V., Pudžiuvelytė, L., Bernatonienė, J., & Jurevičius, J. (2023). Elsholtzia ciliata essential oil exhibits a smooth muscle relaxant effect. Pharmaceuticals, 16(10), 1464. https://doi.org/10.3390/ph16101464
  31. Mohammed, H.A., Sulaiman, G.M., Khan, R.A., & Al-Saffar, A. (2024). Essential oils pharmacological activity: Chemical markers, biogenesis, plant sources, and commercial products. Process Biochemistry, 144, 112-132. https://doi.org/10.1016/j.procbio.2024.05.021
  32. Nikkhah, E., Asnaashari, S., Babaei, H., Afshar, F.H., & Delazar, A. (2017). Chemical composition and biological activities of essential oil and methanol extract of Scrophularia umbrosa. Research Journal of Pharmacognosy, 4(1), 41-50.
  33. Pereira, S.L., Marques, A.M., Sudo, R.T., Kaplan, M.A., & Zapata-Sudo, G. (2013). Vasodilator activity of the essential oil from aerial parts of Pectis brevipedunculata and its main constituent citral in rat aorta. Molecules, 18(3), 3072-3085. https://doi.org/10.3390/molecules18033072
  34. Pires, A.F., Madeira, S.V., Soares, P.M., Montenegro, C.M., Souza, E.P., Resende, A.C., Soares de Moura, R., Assreuy, A.M., & Criddle, D.N. (2012). The role of endothelium in the vasorelaxant effects of the essential oil of Ocimum gratissimum in aorta and mesenteric vascular bed of rats. Canadian Journal of Physiology and Pharmacology, 90(10), 1380 1385. https://doi.org/10.1139/y2012-095
  35. Rasheed, H.M., Khan, T., Wahid, F., Khan, R., & Shah, A.J. (2015). Chemical composition and vasorelaxant and antispasmodic effects of essential oil from Rosa indica L. petals. Evidence-Based Complementary and Alternative Medicine, 2015, 279247. https://doi.org/10.1155/2015/279247
  36. Rasheed, H.M., Khan, T., Wahid, F., Khan, R., & Shah, A.J. (2016). Chemical composition and vascular and intestinal smooth muscle relaxant effects of the essential oil from Psidium guajava fruit. Pharmaceutical Biology, 54(11), 2679-2684. https://doi.org/10.1080/13880209.2016.1178309
  37. Raut, J.S., & Karuppayil, S.M. (2014). A status review on the medicinal properties of essential oils. Industrial Crops and Products, 62, 250-264. https://doi.org/10.1016/j.indcrop.2014.05.055
  38. Sailaja, G.R., Sriramavaratharajan, V., Murugan, R., Mallavarapu, G.R., & Chellappan, D.R. (2021). Vasorelaxant property of Plectranthus vettiveroides root essential oil and its possible mechanism. Journal of Ethnopharmacology, 274, 114048. https://doi.org/10.1016/j.jep.2021.114048
  39. Sargazi Zadeh, G., & Panahi, N. (2017). Endothelium-independent vasorelaxant activity of Trachyspermum ammi essential oil on rat aorta. Clinical and Experimental Hypertension, 39(2), 133-138. https://doi.org/10.1080/10641963.2016.1235178
  40. Shiva Kumar, A., Jeyaprakash, K., Chellappan, D. R., & Murugan, R. (2017). Vasorelaxant and cardiovascular properties of the essential oil of Pogostemon elsholtzioides. Journal of Ethnopharmacology, 199, 86-90. https://doi.org/10.1016/j.jep.2017.01.036
  41. Silva, F.S., Menezes, P.M., de Sá, P.G., Oliveira, A.L., Souza, E.A., Almeida, J.R., de Lima, J.T., Uetanabaro, A.P., Silva, T.R., Peralta, E.D., & Lucchese, A.M. (2016). Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides. Pharmaceutical Biology, 54(1), 25-34. https://doi.org/10.3109/13880209.2015.1005751
  42. Sivakumar, L., Chellappan, D.R., Sriramavaratharajan, V., & Murugan, R. (2020). Root essential oil of Chrysopogon zizanioides relaxes rat isolated thoracic aorta - an ex vivo approach. Zeitschrift fur Naturforschung. C, Journal of Biosciences, 76(3-4), 161-168. https://doi.org/10.1515/znc-2020-0143
  43. Soyingbe, O.S., Makhafola, T.J., Mahlobo, B.P., Salahdeen, H.M., Lawal, O.A., & Opoku, A.R. (2017). Antiasthma activity of Eucalyptus grandis essential oil and its main constituent: Vasorelaxant effect on aortic smooth muscle isolated from nomotensive rats. Journal of Experimental and Applied Animal Sciences, 2(2), 211-2022. https://doi.org/10.20454/jeaas.2017.1287
  44. Spadaro, F., Costa, R., Circosta, C., & Occhiuto, F. (2012). Volatile composition and biological activity of key lime Citrus aurantifolia essential oil. Natural Product Communications, 7(11), 1523-1526.
  45. Tao, L., Hu, H.S., & Shen, X.C. (2013). Endothelium-dependent vasodilatation effects of the essential oil from Fructus alpiniae zerumbet (EOFAZ) on rat thoracic aortic rings in vitro. Phytomedicine, 20(5), 387-393. https://doi.org/10.1016/j.phymed.2012.12.014
  46. Taşkor Önel, G., & Yaman Akbay, H.G. (2022). Uçucu ve sabit yağlar: Kimyasal yapı-aktivite ilişki değerlendirmesi [Volatile and fixed oils: Evaluation of chemical structure-activity relationship]. Bayburt University Journal of Science, 5(1), 104-114. https://doi.org/10.55117/bufbd.1021436
  47. Tognolini, M., Ballabeni, V., Bertoni, S., Bruni, R., Impicciatore, M., & Barocelli, E. (2007). Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis. Pharmacological Research, 56(3), 254-260. https://doi.org/10.1016/j.phrs.2007.07.002
  48. Vora, L.K., Gholap, A.D., Hatvate, N.T., Naren, P., Khan, S., Chavda, V.P., Balar, P.C., Gandhi, J., & Khatri, D.K. (2024). Essential oils for clinical aromatherapy: A comprehensive review. Journal of Ethnopharmacology, 330, 118180. https://doi.org/10.1016/j.jep.2024.118180
  49. Xu, J.F., Xia, J., Wan, Y., Yang, Y., Wu, J.J., Peng, C., & Ao, H. (2022). Vasorelaxant activities and its underlying mechanisms of magnolia volatile oil on rat thoracic aorta based on network pharmacology. Frontiers in Pharmacology, 13, 812716. https://doi.org/10.3389/fphar.2022.812716
  50. Yvon, Y., Raoelison, E.G., Razafindrazaka, R., Randriantsoa, A., Romdhane, M., Chabir, N., Mkaddem, M.G., & Bouajila, J. (2012). Relation between chemical composition or antioxidant activity and antihypertensive activity for six essential oils. Journal of Food Science, 77(8), H184-H191. https://doi.org/10.1111/j.1750-3841.2012.02812.x
  51. Żukowska, G., & Durczyńska, Z. (2024). Properties and applications of essential oils: A review. Journal of Ecological Engineering, 25(2), 333-340. https://doi.org/10.12911/22998993/177404