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Journals / Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi / 2016 / Cilt: 17 Sayı: 2

Determination of some biological activities of Tricholoma terreum and Coprinus micaceus

Tricholoma terreum ve Coprinus micaceus’un bazı biyolojik aktivitelerinin belirlenmesi

Hasan Akgül
Aylin Dilara Nur
Mustafa Sevindik
Muhittin Doğan
Pages
158–162
Publication Date
2016-06-24
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of the study, antioxidant and DNA protective activities of ethanol extracts of both mushroom species were not showed any notable effect and total oxidant value was determined very high.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The use of mushrooms as food also as medicine is increasing day by day.
📊
Key Findings & Data:DNA protective activity was determined by using the supercoiled pBR322 DNA.
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Conclusion & Impact:As a result of the study, antioxidant and DNA protective activities of ethanol extracts of both mushroom species were not showed any notable effect and total oxidant value was determined very high.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Tricholoma terreum · Coprinus micaceus · Mineral Madde

Abstract

The use of mushrooms as food also as medicine is increasing day by day. In this study, an edible mushrooms - Tricholoma terreum (Schaeff.) P. Kumm. and Coprinus micaceus (Bull.) Fr. – were used as materials. This study was aimed to determine the antioxidant and DNA protective activity of ethanol extract of mushrooms and determine and compare total antioxidant, oxidative stress index with oxidative levels and Fe, Mg, Zn, Cu, Na and Ca contents. The antioxidant activity of mushrooms were analyzed with using DPPH and total antioxidant and oxidative levels were analyzed with using Rel Assay Diagnostics kits. DNA protective activity was determined by using the supercoiled pBR322 DNA. Furthermore, element contents of the mushrooms were determined with wet decomposition method by using atomic absorption spectrophotometry. As a result of the study, antioxidant and DNA protective activities of ethanol extracts of both mushroom species were not showed any notable effect and total oxidant value was determined very high.

Özet

Mantarların gıda olarak kullanımlarının yanı sıra tıbbi açıdan kullanımları gün geçtikçe artmaktadır. Bu çalışmada yenilebilir mantar türlerinden Tricholoma terreum (Schaeff.) P. Kumm. ve Coprinus micaceus (Bull.) Fr. materyal olarak kullanılmıştır. Çalışmada mantarların etanol özütlerinin antioksidan ve DNA koruyucu aktivitelerinin belirlenmesi ve toplam antioksidan ve oksidan seviyeleriyle oksidatif stres indeksleri ve Fe, Mg, Zn, Cu, Na ve Ca içeriklerinin tespiti ve kıyaslanması amaçlanmıştır. Antioksidan aktiviteleri DPPH metodu ile toplam antioksidan ve oksidan seviyeleri ise Rel Assay Diagnostics kitleri kullanılarak belirlenmiştir. DNA koruyucu aktivitesi pBR322 süperkoil DNA kullanılarak belirlenmiştir. Ayrıca element içerikleri yaş yakma metodu ile atomik absorpsiyon spektrofotometresi kullanılarak saptanmıştır. Çalışma sonucunda her iki mantar türünün etanol özütleri antioksidan ve DNA koruyucu aktivitelerinin dikkate değer bir etki göstermemiştir ve toplam oksidan değerlerinin çok yüksek olduğu belirlenmiştir.

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Cite This Article

Akgül, H., Nur, A. D., Sevindik, M., Doğan, M. (2016). Determination of some biological activities of Tricholoma terreum and Coprinus micaceus. Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, Cilt: 17 Sayı: 2, 158–162. https://doi.org/10.17474/acuofd.63466

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