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Journals / Anatolian Journal of Botany / 2026 / Cilt: 10 Sayı: 2

Integrated in vitro and in silico evaluation of the antibiofilm activity of Artemisia dracunculus L. against Pseudomonas aeruginosa

Artemisia dracunculus L.’un Pseudomonas aeruginosa’ya Karşı Antibiyofilm Etkinliğinin Entegre In Vitro ve In Silico Değerlendirmesi: Quorum Sensing ile İlişkili Hedef Proteinlerin Moleküler Yerleştirme Analizi

Suzan Sahin Dogan(KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ)0000-0002-1554-0080
Pages
243–249
Publication Date
2026-10-12
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Overall, the results demonstrate that Artemisia dracunculus represents a promising natural source of bioactive compounds for controlling biofilm-associated infections.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study investigated the antibiofilm and antibacterial potential of Artemisia dracunculus L. extract through integrated in vitro and in silico approaches.
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Key Findings & Data:In terms of antibacterial activity, the strongest effect was detected against the Gram-positive bacterium Bacillus subtilis, with an inhibition zone diameter of 9.95±0.45 mm.
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Conclusion & Impact:Overall, the results demonstrate that Artemisia dracunculus represents a promising natural source of bioactive compounds for controlling biofilm-associated infections.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antibiofilm · Artemisia dracunculus · Quorum Sensing

Abstract

This study investigated the antibiofilm and antibacterial potential of Artemisia dracunculus L. extract through integrated in vitro and in silico approaches. Antibacterial activity was evaluated using the disc diffusion method against Pseudomonas aeruginosa, Bacillus subtilis, and Bacillus licheniformis while antibiofilm activity was determined by crystal violet assay. The extract exhibited promising antibiofilm activity at the tested concentration against Pseudomonas aeruginosa. In terms of antibacterial activity, the strongest effect was detected against the Gram-positive bacterium Bacillus subtilis, with an inhibition zone diameter of 9.95±0.45 mm. To explore the possible molecular basis of these effects, chlorogenic acid and kaempferol were subjected to molecular docking analyses against LasR (transcriptional regulator) and anthranilate–coenzyme A ligase (PqsA). Chlorogenic acid and kaempferol showed favorable binding affinities, with docking scores ranging from -7.27 to –9.23 kcal/mol, indicating strong interactions with the active sites of both proteins. Overall, the results demonstrate that Artemisia dracunculus represents a promising natural source of bioactive compounds for controlling biofilm-associated infections. Further studies involving compound isolation, mechanistic confirmation, and in vivo validation are recommended.

Özet

Bu çalışmada, Artemisia dracunculus L. özütünün antibiyofilm ve antibakteriyel potansiyeli, entegre in vitro ve in silico yaklaşımlar kullanılarak incelenmiştir. Antibakteriyel aktivite, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus licheniformis'e karşı disk difüzyon yöntemi kullanılarak değerlendirilirken, antibiyofilm aktivitesi kristal viyole testi ile belirlenmiştir. Ekstrakt, test edilen konsantrasyonda Pseudomonas aeruginosa'ya karşı umut verici antibiyofilm aktivitesi sergilemiştir. Antibakteriyel aktivite açısından, en güçlü etki, 9.95±0.45 mm'lik bir inhibisyon bölgesi çapıyla Gram-pozitif bakteri Bacillus subtilis'e karşı tespit edilmiştir. Bu etkilerin olası moleküler temelini araştırmak amacıyla, klorojenik asit ve kaempferol, LasR (transkripsiyon regülatör) ve antranilat-koenzim A ligazı (PqsA) ile moleküler kenetlenme analizlerine tabi tutulmuştur. Klorojenik asit ve kaempferol, -7.27 ile -9.23 kcal/mol arasında değişen kenetlenme puanlarıyla olumlu bağlanma afiniteleri göstermiş ve bu da her iki proteinin aktif bölgeleriyle güçlü etkileşimler olduğunu göstermiştir. Genel olarak, sonuçlar Artemisia dracunculus L.'un biyofilmle ilişkili enfeksiyonları kontrol etmek için umut verici bir doğal biyoaktif bileşik kaynağı olduğunu göstermektedir. Bileşik izolasyonu, mekanizma doğrulaması ve in vivo validasyonu içeren daha ileri çalışmalar önerilmektedir.

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Cite This Article

Dogan, S. S. (2026). Integrated in vitro and in silico evaluation of the antibiofilm activity of Artemisia dracunculus L. against Pseudomonas aeruginosa. Anatolian Journal of Botany, Cilt: 10 Sayı: 2, 243–249. https://doi.org/10.30616/ajb.1962327

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