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Journals / Anatolian Journal of Botany / 2026 / Cilt: 10 Sayı: 2

Antimicrobial activity of green-synthesized MgO nanoparticles by using Sideritis perfoliata and Thymbra spicata var. spicata extracts

Sideritis perfoliata ve Thymbra spicata var. spicata ekstraktları kullanılarak yeşil sentez yöntemiyle üretilen MgO nanopartiküllerinin antimikrobiyal aktiviteleri

Kağan Veryer(OSMANIYE KORKUT ATA UNIVERSITY)0000-0002-0227-1619
Yusuf Zalaoğlu(OSMANIYE KORKUT ATA UNIVERSITY)0000-0003-0193-799X
Nazmi Sedefoğlu(OSMANIYE KORKUT ATA UNIVERSITY)0000-0001-5364-7375
Çağrı Çot(OSMANIYE KORKUT ATA UNIVERSITY)0009-0007-5431-3626
Duygu Gödelek(OSMANIYE KORKUT ATA UNIVERSITY)0000-0002-4933-0305
Ayşe Sağır(OSMANIYE KORKUT ATA UNIVERSITY)0009-0003-0404-1942
Fuat Bozok(OSMANIYE KORKUT ATA UNIVERSITY)0000-0002-9370-7712
Pages
223–228
Publication Date
2026-10-09
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The present study is the first report on the antibacterial activity of green-synthesized MgO NPs by using extracts of S. perfoliata and T. spicata var. spicata collected from Osmaniye province (The East Mediterranean Region) of Türkiye

📌 3 Key Takeaways

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Objective & Hypothesis:In the present study, MgO nanoparticles (NPs) were green-synthesized using Sideritis perfoliata (SPE) and Thymbra spicata var. spicata (TSE) extracts.
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Key Findings & Data:MgO nanoparticles produced using SPE and TSE extracts were found to have noticeably different sizes, approximately 80 and 250 nm, respectively.
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Conclusion & Impact:The present study is the first report on the antibacterial activity of green-synthesized MgO NPs by using extracts of S. perfoliata and T. spicata var. spicata collected from Osmaniye province (The East Mediterranean Region) of Türkiye
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antibakteriyel · nanopartikül · Sideritis

Abstract

In the present study, MgO nanoparticles (NPs) were green-synthesized using Sideritis perfoliata (SPE) and Thymbra spicata var. spicata (TSE) extracts. XRD and SEM analyses were used for the characterization of these NPs. Furthermore, green-synthesized MgO NPs were evaluated against various strains (E. faecalis, Y. enterocolitica, E. coli, S. aureus, P. aeruginosa, A. baumannii) to determine antibacterial activity. XRD data reveal the polycrystalline cubic structure of the green-synthesized MgO NPs. SEM images of MgO NPs produced with SPE and TSE showed that they had smooth surfaces and approximately spherical and crescent-like forms, respectively. MgO nanoparticles produced using SPE and TSE extracts were found to have noticeably different sizes, approximately 80 and 250 nm, respectively. Additionally, the antibacterial responses of the tested strains varied depending on the plant extract used, with the largest inhibition zones observed against A. baumannii for TSE-derived MgO NPs (13.0 ± 1.0 mm) and E. faecalis for SPE-derived MgO NPs (9.0 ± 0.5 mm). The present study is the first report on the antibacterial activity of green-synthesized MgO NPs by using extracts of S. perfoliata and T. spicata var. spicata collected from Osmaniye province (The East Mediterranean Region) of Türkiye

Özet

Bu çalışmada, Sideritis perfoliata (SPE) ve Thymbra spicata var. spicata (TSE) ekstraktları kullanılarak MgO nanopartikülleri (NP’ler) yeşil sentez yöntemiyle üretilmiştir. Bu NP’lerin karakterizasyonunda XRD ve SEM analizleri kullanılmıştır. Ayrıca, yeşil sentezlenen MgO NP’lerin antibakteriyel aktivitesini belirlemek amacıyla çeşitli bakteri suşlarına (E. faecalis, Y. enterocolitica, E. coli, S. aureus, P. aeruginosa ve A. baumannii) karşı etkileri değerlendirilmiştir. XRD verileri, yeşil sentezlenen MgO NP’lerin polikristalin kübik yapıda olduğunu göstermiştir. SPE ve TSE kullanılarak üretilen MgO NP’lerin SEM görüntüleri, sırasıyla pürüzsüz yüzeyli, yaklaşık küresel ve hilal benzeri morfolojilere sahip olduklarını göstermiştir. SPE ve TSE ekstraktları kullanılarak üretilen MgO nanopartiküllerinin sırasıyla yaklaşık 80 ve 250 nm olmak üzere belirgin biçimde farklı boyutlara sahip olduğu belirlenmiştir. Ayrıca, test edilen suşların antibakteriyel yanıtlarının kullanılan bitki ekstraktına bağlı olarak değiştiği; en yüksek inhibisyon zonlarının TSE kökenli MgO NP’lerde A. baumannii’ye karşı 13,0 ± 1,0 mm ve SPE kökenli MgO NP’lerde E. faecalis’e karşı 9,0 ± 0,5 mm olarak gözlendiği belirlenmiştir. Bu çalışma, Türkiye’nin Doğu Akdeniz Bölgesi’nde yer alan Osmaniye ilinden toplanan S. perfoliata ve T. spicata var. spicata ekstraktları kullanılarak yeşil sentezlenen MgO NP’lerin antibakteriyel aktivitesine ilişkin ilk rapordur.

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Cite This Article

Veryer, K., Zalaoğlu, Y., Sedefoğlu, N., Çot, Ç., Gödelek, D., Sağır, A., Bozok, F. (2026). Antimicrobial activity of green-synthesized MgO nanoparticles by using Sideritis perfoliata and Thymbra spicata var. spicata extracts. Anatolian Journal of Botany, Cilt: 10 Sayı: 2, 223–228. https://doi.org/10.30616/ajb.1989756

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References (41)

  1. Abdallah Y, Ogunyemi SO, Abdelazez A, Zhang M, Hong X, Ibrahim E, Hossain A, Fouad H, Li B, Chen J (2019). The green synthesis of MgO nano-flowers using Rosmarinus officinalis L. (Rosemary) and the antibacterial activities against Xanthomonas oryzae pv. oryzae. BioMed Research International 2019: 5620989.
  2. Abinaya S, Kavitha HP, Prakash M, Muthukrishnaraj A (2021). Green synthesis of magnesium oxide nanoparticles and its applications: A review. Sustainable Chemistry and Pharmacy 19: 100368.
  3. Alfaro A, León A, Guajardo-Correa E, Reúquen P, Torres F, Mery M, Segura R, Zapata PA, Orihuela PA (2019). MgO nanoparticles coated with polyethylene glycol as carrier for 2-Methoxyestradiol anticancer drug. PLoS One 14: e0214900.
  4. Anicˇić N, Vukomanović M, Koklič T, Suvorov D (2018). Fewer Defects in the Surface Slows the Hydrolysis Rate, Decreases the ROS Generation Potential, and Improves the Non-ROS Antimicrobial Activity of MgO. Small 14: 1800205.
  5. Balakrishnan G, Velavan R, Batoo KM, Raslan EH (2020). Microstructure, optical and photocatalytic properties of MgO nanoparticles. Results in Physics 16: 103013.
  6. Dabhane H, Ghotekar S, Tambade P, Pansambal S, Oza R, Medhane V (2021). MgO nanoparticles: Synthesis, characterization, and applications as a catalyst for organic transformations. European Journal of Chemistry 12: 86-108.
  7. Davis P (1982). Flora of Turkey and the East Aegean Islands, Vol. 7. Edinburgh: University Press.
  8. Dogan T (2026). Assessment of the Wastewater Treatment Performance of Neodymium-Doped Al2O3 Nanoparticles Under UV Irradiation. Applied Sciences 16: 3773.
  9. El-Sayyad GS, Mosallam FM, El-Batal AI (2018). One-pot green synthesis of magnesium oxide nanoparticles using Penicillium chrysogenum melanin pigment and gamma rays with antimicrobial activity against multidrug-resistant microbes. Advanced Powder Technology 29: 2616-2625.
  10. Gibson A, Haydock R, LaFemina JP (1993). The electronic structure of neutral and charged surface vacancy defects in periclase. Applied Surface Science 72: 285-293.
  11. Gunalan S, Sivaraj R, Rajendran V (2012). Green synthesized ZnO nanoparticles against bacterial and fungal pathogens. Progress in Natural Science: Materials International 22: 693-700.
  12. Hornak J, Trnka P, Kadlec P, Michal O, Mentlík V, Šutta P, Csányi GM, Tamus ZÁ (2018). Magnesium oxide nanoparticles: Dielectric properties, surface functionalization and improvement of epoxy-based composites insulating properties. Nanomaterials 8: 381.
  13. Hoseinnejad M, Jafari SM, Katouzian I (2018). Inorganic and metal nanoparticles and their antimicrobial activity in food packaging applications. Crit. Rev. Microbiol. 44: 161-181.
  14. Idris YJ, Tijani JO, Mustapha S, Ndamitso MM, Abdulkareem AS, Egbosiuba TC, Sagadevan S (2024). Effective removal of organic and inorganic pollutants from abattoir wastewater using magnesium oxide/activated carbon nanocomposites. Ceram. Int. 50: 25933-25947.
  15. Jansson S, Brabie V, Jönsson P (2005). Corrosion mechanism and kinetic behaviour of MgO–C refractory material in contact with CaO–Al2O3–SiO2–MgO slag. Scand. J. Metall. 34: 283-292.
  16. Karthik K, Dhanuskodi S, Gobinath C, Prabukumar S, Sivaramakrishnan S (2019). Fabrication of MgO nanostructures and its efficient photocatalytic, antibacterial and anticancer performance. J. Photochem. Photobiol. B: Biol. 190: 8-20.
  17. Lu L, Zhang L, Zhang X, Wu Z, Huan S, Shen G, Yu R (2010). A MgO Nanoparticles Composite Matrix-Based Electrochemical Biosensor for Hydrogen Peroxide with High Sensitivity. Electroanalysis 22: 471-477.
  18. Muzammil S, Hayat S, Fakhar-E-Alam M, Aslam B, Siddique MH, Nisar MA, Saqalein M, Atif M, Sarwar A, Khurshid A (2018). Nanoantibiotics: Future nanotechnologies to combat antibiotic resistance. Front. Biosci. 10: 352-374.
  19. Park W, Na K (2015). Advances in the synthesis and application of nanoparticles for drug delivery. WIREs Nanomedicine and Nanobiotechnology 7: 494-508.
  20. Parvizi E, Tayebee R, Koushki E (2019). Mg-doped ZnO and Zn-doped MgO semiconductor nanoparticles; synthesis and catalytic, optical and electro-optical characterization. Semiconductors 53: 1769-1783.
  21. Periakaruppan R, Naveen V, Danaraj J (2022). Green Synthesis of Magnesium Oxide Nanoparticles with Antioxidant Potential Using the Leaf Extract of Piper nigrum. JOM 74: 4817-4822.
  22. Pugazhendhi A, Prabhu R, Muruganantham K, Shanmuganathan R, Natarajan S (2019). Anticancer, antimicrobial and photocatalytic activities of green synthesized magnesium oxide nanoparticles using aqueous extract of Sargassum wightii. Journal of Photochemistry and Photobiology B: Biology 190: 86-97.
  23. Qiao S, Novitskaya E, Ren T, Pena G, Graeve OA (2019). Phase and Morphology Control of Magnesium Nanoparticles via Lithium Doping. Crystal Growth & Design 19: 3626-3632.
  24. Ramezani Farani M, Farsadrooh M, Zare I, Gholami A, Akhavan O (2023). Green Synthesis of Magnesium Oxide Nanoparticles and Nanocomposites for Photocatalytic Antimicrobial, Antibiofilm and Antifungal Applications. Catalysts 13: 642.
  25. Rashid Khan M, Omar Alafaleq N, Kumar Ramu A, Alhosaini K, Shahnawaz Khan M, Zughaibi TA, Tabrez S (2024). Evaluation of biogenically synthesized MgO NPs anticancer activity against breast cancer cells. Saudi J. Biol. Sci. 31: 103874.
  26. Rodriguez JA, Jirsak T, Chaturvedi S (1999). Reaction of H2S with MgO(100) and Cu/MgO(100) surfaces: Band-gap size and chemical reactivity. The Journal of Chemical Physics 111: 8077-8087.
  27. Salehi M, Maleksaeedi S, Nai MLS, Gupta M (2019). Towards additive manufacturing of magnesium alloys through integration of binderless 3D printing and rapid microwave sintering. Additive Manufacturing 29: 100790.
  28. Sedefoglu N (2023). Characterization and photocatalytic activity of ZnO nanoparticles by green synthesis method. Optik 288: 171217.
  29. Sedefoglu N, Er S, Veryer K, Zalaoglu Y, Bozok F (2023a). Green synthesized CuO nanoparticles using macrofungi extracts: Characterization, nanofertilizer and antibacterial effects. Materials Chemistry and Physics 309: 128393.
  30. Sedefoglu N, Erdogan NH, Kutlu T, Kavak H (2023b). Tailoring Sb doping concentration to achieve p-type nanostructured ZnO thin film grown by sol–gel method. Journal of Materials Science: Materials in Electronics 34: 232.
  31. Sedefoglu N, Veryer K, Zalaoglu D, Bozok F (2025). Antibacterial and photocatalytic performances of bioactive In-doped ZnO nanoparticles. Mater Sci Eng B 313: 117914.
  32. Sedefoglu N, Zalaoglu Y, Bozok F (2022). Green synthesized ZnO nanoparticles using Ganoderma lucidum: Characterization and In Vitro Nanofertilizer effects. J. Alloys Compd. 918: 165695.
  33. Selim YA, Azb MA, Ragab I, Abd El-Azim MHM (2020). Green Synthesis of Zinc Oxide Nanoparticles Using Aqueous Extract of Deverra tortuosa and their Cytotoxic Activities. Sci. Rep. 10: 3445.
  34. Shang Y, Hasan MK, Ahammed GJ, Li M, Yin H, Zhou J (2019). Applications of Nanotechnology in Plant Growth and Crop Protection: A Review. Molecules 24: 2558.
  35. Suresh J, Pradheesh G, Alexramani V, Sundrarajan M, Hong SI (2018). Green synthesis and characterization of hexagonal-shaped MgO nanoparticles using insulin plant (Costus pictus D. Don) leaf extract and its antimicrobial as well as anticancer activity. Advanced Powder Technology 29: 1685-1694.
  36. Suresh J, Yuvakkumar R, Sundrarajan M, Hong SI (2014). Green synthesis of magnesium oxide nanoparticles. Advanced Materials Research 952: 141-144.
  37. Umaralikhan L, Jamal Mohamed Jaffar M (2018). Green Synthesis of MgO Nanoparticles and it Antibacterial Activity. Iranian Journal of Science and Technology, Transactions A: Science 42: 477-485.
  38. Verma SK, Nisha K, Panda PK, Patel P, Kumari P, Mallick MA, Sarkar B, Das B (2020). Green synthesized MgO nanoparticles infer biocompatibility by reducing in vivo molecular nanotoxicity in embryonic zebrafish through arginine interaction elicited apoptosis. Sci. Total Environ. 713: 136521.
  39. Vidhya E, Vijayakumar S, Nilavukkarasi M, Punitha VN, Snega S, Praseetha PK (2021). Green fabricated MgO nanoparticles as antimicrobial agent: Characterization and evaluation. Materials Today: Proceedings 45: 5579-5583.
  40. Vijayakumar S, Punitha VN, Parameswari N (2022). Phytonanosynthesis of MgO Nanoparticles: Green Synthesis, Characterization and Antimicrobial Evaluation. Arabian Journal for Science and Engineering 47: 6729-6734.
  41. Youssef AM, El-Sayed SM (2018). Bionanocomposites materials for food packaging applications: Concepts and future outlook. Carbohydrate Polymers 193: 19-27.