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Journals / NATURENGS / 2026 / Volume: 7 Issue: 1

Screening of Phosphate-Solubilizing and Potential Nitrogen-Fixing Bacteria from Apple Orchards in Karaman

Suzan Sahin Dogan(KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ)0000-0002-1554-0080
Aytaç Kocabaş(KARAMANOĞLU MEHMETBEY ÜNİVERSİTESİ)0000-0001-7622-1932
Journal
NATURENGS
Pages
—
Publication Date
2026-10-27
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results indicate that apple orchard soil is a valuable source of bacteria with functional traits potentially associated with plant nutrient cycling.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to isolate and molecularly identify bacteria capable of growing in nitrogen-free medium and solubilizing phosphate from apple orchard soil.
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Key Findings & Data:Taxonomic identification of the isolates was performed based on sequencing of the V4 region of the 16S rRNA gene.
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Conclusion & Impact:The results indicate that apple orchard soil is a valuable source of bacteria with functional traits potentially associated with plant nutrient cycling.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:phosphate solubility · potential nitrogen fixation · bacterial isolates

Abstract

The aim of this study was to isolate and molecularly identify bacteria capable of growing in nitrogen-free medium and solubilizing phosphate from apple orchard soil. Bacterial isolates were recovered from apple orchard soil, and those capable of growing on nitrogen-free medium and solubilizing phosphate were identified. Taxonomic identification of the isolates was performed based on sequencing of the V4 region of the 16S rRNA gene. A total of eight bacterial isolates capable of growing in nitrogen-free medium and 13 phosphate-solubilizing bacterial isolates were obtained. The isolates capable of growing in nitrogen-free medium were affiliated with the genera Pantoea, Streptomyces, Pseudomonas, Agrobacterium, Variovorax, and Rhizobium. The phosphate-solubilizing isolates were affiliated with the genera Pantoea, Enterobacter, Agrobacterium, Acinetobacter, Pseudomonas, Bacillus, Rhizobium, Streptomyces, and Sphingobacterium. Notably, Pseudomonas-related isolates represented a substantial proportion of the phosphate-solubilizing bacterial collection. The results indicate that apple orchard soil is a valuable source of bacteria with functional traits potentially associated with plant nutrient cycling. In particular, isolates capable of solubilizing phosphate and growing in nitrogen-free medium may represent promising candidates for further evaluation as plant growth-promoting microorganisms. These indigenous isolates could be further investigated for their potential use as microbial inoculants to support sustainable agricultural production.

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Cite This Article

Dogan, S. S., Kocabaş, A. (2026). Screening of Phosphate-Solubilizing and Potential Nitrogen-Fixing Bacteria from Apple Orchards in Karaman. NATURENGS, Volume: 7 Issue: 1. https://doi.org/10.46572/naturengs.2022782

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