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Journals / Harran Tarım ve Gıda Bilimleri Dergisi / 2026 / Cilt: 28 Sayı: 2

Isolation and identification of Xanthomonas citri subsp. malvacearum, cotton bacterial blight disease agent and determination of the antibacterial activity of various plant essential oils

Pamuk bakteriyel yanıklık etmeni Xanthomonas citri subsp. malvacearum’un izolasyonu, tanılanması ve farklı bitki uçucu yağlarının antibakteriyel etkinliğinin belirlenmesi

Soner Soylu(Hatay Mustafa Kemal Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü)0000-0003-1002-8958
Merve Kara(HATAY MUSTAFA KEMAL UNIVERSITY)0000-0001-7320-3376
Yusuf Gümüş(HATAY MUSTAFA KEMAL UNIVERSITY)0000-0001-7818-1831
Emine Mine Soylu(HATAY MUSTAFA KEMAL UNIVERSITY)0000-0001-5961-0848
Pages
180–191
Publication Date
2024-06-21
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results showed that essential oils of Thymus spp., Thymbra spp. and Origanum spp. have the potential to be used as an alternative to chemical control against seed-borne bacterial plant pathogens.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Cotton is one of the most important industrial crops produced in large areas in Turkey.
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Key Findings & Data:In this study, the bacterial pathogen X. citri subsp. malvacearum was isolated from cotton plants showing typical disease symptoms during the 2023 growing season in Diyarbakır provinces and the antibacterial effect of essential oils obtained from 11 different medicinal plants (Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana and Thymbra spicata) were determined by disk diffusion method.
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Conclusion & Impact:The results showed that essential oils of Thymus spp., Thymbra spp. and Origanum spp. have the potential to be used as an alternative to chemical control against seed-borne bacterial plant pathogens.
🔬Design:Araştırma Makalesi
📍Scope:2023 Verileri
⚙️Variables:pamuk · bakteriyel yanıklık · Xanthomonas

Abstract

Cotton is one of the most important industrial crops produced in large areas in Turkey. Cotton bacterial blight disease agent Xanthomonas citri subsp. malvacearum (Syn. Xanthomonas axonopodis pv. malvacearum) is a seed-borne pathogen. Plant essential oils are natural antimicrobial compounds that have the potential to be used as an alternative to chemical control of seed-borne phytopathogens. In this study, the bacterial pathogen X. citri subsp. malvacearum was isolated from cotton plants showing typical disease symptoms during the 2023 growing season in Diyarbakır provinces and the antibacterial effect of essential oils obtained from 11 different medicinal plants (Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana and Thymbra spicata) were determined by disk diffusion method. Bacterial isolates, obtained from cotton plants showing typical disease symptoms, were identified as X. citri subsp. malvacearum by biochemical and pathogenicity tests and also MALDI-TOF analysis. The essential oils used in the study showed antibacterial activity against the bacterial agent by forming an inhibition zone with a diameter of 6.33-46.33 mm in the nutrient media. T. serpyllum and T. spicata (43.33 mm), O. syriacum (43.00 mm), T. syriacus (38.33 mm) and O. onites (37.3 mm), were identified as the most effective essential oils respectively. The essential oils of H. perforatum (9.67 mm), L. nobilis (9.0 mm) and S. aramiensis (6.33 mm) showed relatively lower antibacterial activity against the disease agent. The results showed that essential oils of Thymus spp., Thymbra spp. and Origanum spp. have the potential to be used as an alternative to chemical control against seed-borne bacterial plant pathogens.

Özet

Pamuk Türkiye’de geniş alanlarda üretimi yapılan önemli endüstri bitkilerin başında gelir. Pamuk bakteriyel yanıklık hastalığı etmeni Xanthomonas citri subsp. malvacearum (Syn. Xanthomonas axonopodis pv. malvacearum) tohum kökenli bir patojendir. Bitki uçucu yağları tohum kökenli fitopatojen türlere karşı kimyasal mücadeleye alternatif olarak kullanım potansiyeline sahip doğal antimikrobiyal bileşiklerdir. Bu çalışmada, Diyarbakır ilinde 2023 yetiştirme sezonunda tipik hastalık belirtileri gösteren pamuk bitkilerinden X. citri subsp. malvacearum bakteriyel etmenin izolasyonu, tanılanması ve 11 farklı tıbbi bitkiden (Thymus serpyllum, Origanum syriacum, Thymus syriacus, Origanum onites, Cistus laden, Salvia aramiensis, Laurus nobilis, Hypericum perforatum, Rosmarinus officinalis, Origanum majorana ve Thymbra spicata) elde edilen uçucu yağların antibakteriyel etkisi disk difüzyon yöntemi ile belirlenmiştir. Tipik hastalık belirtileri gösteren pamuk bitkilerinden izole edilen izolatlar biyokimyasal ve patojenite testleri ve MALDI-TOF ile tür düzeyinde X. citri subsp. malvacearum olarak tanılanmıştır. Çalışmada kullanılan bitki uçucu yağları besi yerinde 6.33-46.33 mm çapında engelleme zonu oluşturmak suretiyle bakteriyel etmene karşı antibakteriyel etkinlik göstermiştir. En etkili uçucun yağlar sırasıyla T. serpyllum ve T. spicata (43.33 mm), O. syriacum (43.00 mm), T. syriacus (38.33 mm) ve O. onites (37.3 mm) olarak belirlenmiştir. H. perforatum (9.67 mm), L. nobilis (9.0 mm) ve S. aramiensis (6.33 mm) bitki uçucu yağları hastalık etmene karşı nispeten daha düşük oranlarda antibakteriyel etkinlik göstermiştir. Elde edilen sonuçlar Thymus spp., Thymbra spp. ve Origanum spp.’ye ait bitki uçucu yağlarının tohum kökenli bitki patojeni bakteriyel hastalık etmenine karşı kimyasal mücadeleye alternatif mücadele yöntemi olarak uygulanma potansiyeline sahip olduğunu göstermiştir.

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Cite This Article

Soylu, S., Kara, M., Gümüş, Y., Soylu, E. M. (2024). Isolation and identification of Xanthomonas citri subsp. malvacearum, cotton bacterial blight disease agent and determination of the antibacterial activity of various plant essential oils. Harran Tarım ve Gıda Bilimleri Dergisi, Cilt: 28 Sayı: 2, 180–191. https://doi.org/10.29050/harranziraat.1434729

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