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Journals / Harran Tarım ve Gıda Bilimleri Dergisi / 2026 / Cilt: 30 Sayı: 1

Determination of the in vitro biocontrol potential and mechanisms of action of rhizobacterial antagonist isolates from sugar beet plants against Fusarium culmorum

Şeker pancarı bitkilerinden izole edilen antagonist kökbakteri izolatlarının Fusarium culmorum’a karşı in vitro biyolojik mücadele potansiyelleri ve etki mekanizmalarının belirlenmesi

Murat Öztürk(YOZGAT BOZOK ÜNİVERSİTESİ)0000-0002-9677-3651
Soner Soylu(Hatay Mustafa Kemal University)0000-0003-1002-8958
Şeval Koç(YOZGAT BOZOK ÜNİVERSİTESİ)0009-0005-5892-0517
Emin Aydin(YOZGAT BOZOK ÜNİVERSİTESİ)0000-0002-7853-8885
Sevim Atmaca(YOZGAT BOZOK ÜNİVERSİTESİ)0000-0001-8568-3469
Pages
818–828
Publication Date
2026-03-22
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The in vitro results suggest that Paenibacillus polymyxa 14/7, Bacillus mojavensis 45/2, and Bacillus thuringiensis israelensis 51/3 are the most promising candidates for the development of biological control formulations against F. culmorum in wheat.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Soil-borne fungal pathogens of the Fusarium species cause significant yield and quality losses in economically important crops.
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Key Findings & Data:The in vitro biological control potential of 11 isolates, among 43 potential bacterial isolates obtained from the rhizosphere of sugar beets grown in ten different fields in Yozgat, Turkey, was evaluated in detail.
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Conclusion & Impact:The in vitro results suggest that Paenibacillus polymyxa 14/7, Bacillus mojavensis 45/2, and Bacillus thuringiensis israelensis 51/3 are the most promising candidates for the development of biological control formulations against F. culmorum in wheat.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Antagonist bakteri · Biyolojik mücadele · Buğday

Abstract

Soil-borne fungal pathogens of the Fusarium species cause significant yield and quality losses in economically important crops. Fusarium culmorum (W.G. Smith) Sacc. is a common pathogen associated with crown rot, root rot, and head blight in cereals. Biological control agent (BCA) antagonistic bacterial isolates are a sustainable approach to managing soil-borne pathogens. This study aimed to evaluate the in vitro antifungal potential of rhizobacterial isolates obtained from healthy sugar beet plants against F. culmorum . The in vitro biological control potential of 11 isolates, among 43 potential bacterial isolates obtained from the rhizosphere of sugar beets grown in ten different fields in Yozgat, Turkey, was evaluated in detail. The average inhibition rate of mycelium growth of the fungal pathogen by the 11 selected representative bacterial isolates was 40.45%. The most effective isolates were Paenibacillus polymyxa 14/7 (74.14%), followed by Bacillus mojavensis 45/2 (71.75%) and Bacillus thuringiensis israelensis 51/3 (68.58%). The antagonistic activity of the most effective isolates was associated with a combination of mechanisms, particularly iron competition via siderophores, protease activity causing cell wall degradation, and volatile antifungal ammonia production, rather than a single factor. This study demonstrated that root-associated bacteria isolated from healthy sugar beet plants possess significant in vitro antagonistic potential against F. culmorum . The in vitro results suggest that Paenibacillus polymyxa 14/7, Bacillus mojavensis 45/2, and Bacillus thuringiensis israelensis 51/3 are the most promising candidates for the development of biological control formulations against F. culmorum in wheat.

Özet

Solgunluk ve kök çürüklüğü hastalıklarına neden olan toprak kökenli fungal hastalık etmeni Fusarium türleri ekonomik öneme sahip kültür bitkilerinde ciddi verim ve kalite kayıplarına neden olur. Fusarium culmorum (W.G. Smith) Sacc., tahıllarda Fusarium taç çürüklüğü, kök çürüklüğü ve başak yanıklığı ile ilişkili yaygın bir patojendir. Sağlıklı bitkilerden elde edilen antagonistik bakteriler kullanılarak yapılan biyolojik mücadele, topraktan bulaşan patojenler ile mücadelede sürdürülebilir bir yöntemdir. Bu çalışma, sağlıklı şeker pancarı bitkilerinden elde edilen kök bakteri (rizobakteriyel) izolatların Fusarium culmorum 'a karşı in vitro antifungal potansiyellerini belirlemek amacıyla yapılmıştır. Türkiye'nin Yozgat ilindeki 10 farklı tarlasında yetişen şeker pancarı rizosferlerinden elde edilen 43 potansiyel bakteri izolatı arasından seçilen 11 izolatın in vitro biyolojik mücadele potansiyeli ayrıntılı olarak değerlendirilmiştir. Temsili seçilen 11 bakteri izolatın fungal etmenin misel gelişimini ortalama engelleme oranı %40,45 olarak belirlenmiştir. Misel gelişimini engelleyen en etkili antagonist bakteri izolatı, %74,14 engelleme oranı ile Paenibacillus polymyxa 14/7 olup, bu izolatı %71,75 engelleme oranı ile Bacillus mojavensis 45/2 ve %68,58 engelleme oranı ile Bacillus thuringiensis israelensis 51/3 izolatları izlemiştir. En etkili bakteri izolatların antagonistik etkinliği, tek bir faktörden ziyade, siderofor aracılı demir rekabeti, hücre duvarı bozunmasına neden olan proteaz aktivitesi ve uçucu antifungal amonyak üretimi gibi bir dizi mekanizmanın birleşimi ile bağlantılı olduğu belirlenmiştir. Bu çalışma, sağlıklı şeker pancarı bitkilerinden elde edilen kökbakterilerinin F. culmorum 'a karşı önemli in vitro antagonistik potansiyele sahip olduğunu göstermiştir. In vitro çalışma sonuçları Paenibacillus polymyxa 14/7, Bacillus mojavensis 45/2 ve Bacillus thuringiensis israelensis 51/3 izolatlarının buğdayda F. culmorum 'a karşı biyolojik mücadele formülasyonları geliştirmek için en uygun adaylar olduğunu göstermiştir.

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Cite This Article

Öztürk, M., Soylu, S., Koç, Ş., Aydin, E., Atmaca, S. (2026). Determination of the in vitro biocontrol potential and mechanisms of action of rhizobacterial antagonist isolates from sugar beet plants against Fusarium culmorum. Harran Tarım ve Gıda Bilimleri Dergisi, Cilt: 30 Sayı: 1, 818–828. https://doi.org/10.29050/harranziraat.1845544

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