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Journals / MANTAR DERGİSİ / 2025 / Cilt: 16 Sayı: 2

First Report on Isolating Auricularia nigricans from Shoe Inner Surface: ITS-Based Molecular Identification

Ayakkabı İç Yüzeyinden Auricularia nigricans'nın İzole Edilmesine İlişkin İlk Rapor: ITS Tabanlı Moleküler Tanımlama

Gülçin Özcan Ateş(Çanakkale Onsekiz Mart Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu)0000-0002-8467-2378
Pages
140–145
Publication Date
2025-10-24
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This result reveals that fungi also spread via fomites and their impact on environmentally fungal biodiversity.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:This study aimed to identify a fungus isolated from a shoe sample, one of the personal items that has been in contact with humans.
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Key Findings & Data:Isolation was performed using Dichloran Rose Bengal Chloramphenicol Agar, incubation was carried out at 25°C for 7 days, and then purification was achieved on Potato Dextrose Agar.
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Conclusion & Impact:This result reveals that fungi also spread via fomites and their impact on environmentally fungal biodiversity.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Auricularia nigricans · Ayakkabı · Fomit

Abstract

This study aimed to identify a fungus isolated from a shoe sample, one of the personal items that has been in contact with humans. Isolation was performed using Dichloran Rose Bengal Chloramphenicol Agar, incubation was carried out at 25°C for 7 days, and then purification was achieved on Potato Dextrose Agar. For molecular species identification from pure cultures, the internal transcribed spacer (ITS) region of ribosomal DNA was amplified using primers ITS1 and ITS4 after DNA isolation. The resulting sequence showed a high similarity (99.62%) to Auricularia nigricans in the GenBank database, as determined by BLAST analysis. Phylogenetic analysis also confirmed a strong relationship with this species. While Auricularia nigricans is generally known to be found in dead woody materials in nature, its isolation from a personal item for the first time in this study highlights the potential spread of fomite-borne fungal infections. This result reveals that fungi also spread via fomites and their impact on environmentally fungal biodiversity.

Özet

Bu çalışmada, insan teması olan kişisel eşyalardan biri olan bir ayakkabı numunesinden izole edilen fungus örneğinin tanımlanması amaçlanmıştır. İzolasyon işlemi Dichloran Rose Bengal Chloramphenicol Agar besiyeri kullanılarak gerçekleştirilmiş ve izolasyon petrisi 25 °C'de 7 gün süre inkübe edildi. İnkübasyon sonrası Potato Dextrose Agar besiyerinde saflaştırılmıştır. Saf kültürden moleküler düzeyde tür tayini için DNA izolasyonu sonrası ITS1 ve ITS4 primerleri kullanılarak ribozomal DNA’nın iç transkribe edilmiş aralık bölgesi (ITS) amplifiye edilmiştir. Elde edilen dizi, GenBank veritabanında yapılan BLAST analizi sonucunda Auricularia nigricans türüyle yüksek benzerlik göstermiştir (%99,62). Filogenetik analiz sonuçları da bu türle güçlü bir ilişki olduğunu doğrulamıştır. Auricularia nigricans türü genellikle doğada ölü odunsu materyallerde bulunduğu bilinirken, bu çalışmada ilk kez kişisel bir eşya üzerinden izole edilmesi, fomit kaynaklı mantar bulaşlarının olası yayılım yollarına dikkat çekmektedir. Bu sonuç, fungusların aynı zamanda fomitler aracılığıyla da yayılmasına ve çevresel fungal biyoçeşitliliğine olan etkisini ortaya koymaktadır.

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Cite This Article

Ateş, G. Ö. (2025). First Report on Isolating Auricularia nigricans from Shoe Inner Surface: ITS-Based Molecular Identification. MANTAR DERGİSİ, Cilt: 16 Sayı: 2, 140–145. https://doi.org/10.30708/mantar.1769661

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