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Journals / Lapseki Meslek Yüksekokulu Uygulamalı Araştırmalar Dergisi / 2025 / Cilt: 6 Sayı: 13

Evaluation of Antibacterial Activity of Commercial Lemon, Geranium, and Thistle Oils Against Pseudomonas Species

Ticari Limon, Sardunya (Itır) ve Deve Dikeni Yağlarının Pseudomonas Türlerine Karşı Antibakteriyel Aktivitesinin Değerlendirilmesi

Gülçin Özcan Ateş(Çanakkale Onsekiz Mart Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu)0000-0002-8467-2378
Rana Karli(ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ)0009-0002-1730-8960
Fatma Budak(ÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ)0009-0005-6764-0517
Pages
1–9
Publication Date
2025-12-30
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was determined that thistle fixed oil, lemon, and geranium essential oils did not give an inhibition zone against the tested Pseudomonas species, and their MIC values were greater than 80 μg/mL.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Increasing antibiotic resistance is increasingly affecting human and animal health.
📊
Key Findings & Data:Thus, in this study, the antibacterial activities of commercially available thistle (Silybum marianum) fixed oil, lemon (Citrus limon), and geranium (Pelargonium graveolens) essential oils against Pseudomonas aeruginosa, Pseudomonas putida, and Pseudomonas monteilii isolated from used makeup sponges, as well as Pseudomonas aeruginosa ATCC 27853 and ATCC 10145, were evaluated in vitro using agar well diffusion and microdilution methods.
💡
Conclusion & Impact:It was determined that thistle fixed oil, lemon, and geranium essential oils did not give an inhibition zone against the tested Pseudomonas species, and their MIC values were greater than 80 μg/mL.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Citrus limon · Pelargonium graveolens · Silybum marianum

Abstract

Increasing antibiotic resistance is increasingly affecting human and animal health. Pseudomonas spp. are opportunistic pathogens resistant to multiple antibiotics. Pseudomonas spp. can be found in various environmental sources, including surface water, wastewater, food, and soil, and can cause hospital-associated infections. Therefore, due to the increasing resistance to antibiotics, plants and their extracts are being investigated as alternative natural sources of antibiotics. Thus, in this study, the antibacterial activities of commercially available thistle (Silybum marianum) fixed oil, lemon (Citrus limon), and geranium (Pelargonium graveolens) essential oils against Pseudomonas aeruginosa, Pseudomonas putida, and Pseudomonas monteilii isolated from used makeup sponges, as well as Pseudomonas aeruginosa ATCC 27853 and ATCC 10145, were evaluated in vitro using agar well diffusion and microdilution methods. It was determined that thistle fixed oil, lemon, and geranium essential oils did not give an inhibition zone against the tested Pseudomonas species, and their MIC values were greater than 80 μg/mL.

Özet

Artan antibiyotik direnci insan ve hayvan sağlığını her geçen gün etkilemektedir. Pseudomonas türleri ise birden çok antibiyotiğe karşı dirençli fırsatçı bir patojendir. Pseudomonas türleri yüzey suyu, atık su, gıda ve toprak gibi çevresel kaynaklardan da bulunabilmekte ve hastane ilişkili enfeksiyonlara neden olmaktadır. Bu nedenle artan antibiyotik direnci sebebiyle de antibiyotiklere alternatif doğal kaynaklar olan bitkiler ve onların ekstraktları araştırılmaktadır. Bu nedenle bu çalışmada kullanılmış makyaj süngerlerinden izole edilmiş Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas monteilii izolatları ile Pseudomonas aeruginosa ATCC 27853 ve ATCC 10145’e karşı ticari olarak satılan deve dikeni (Silybum marianum) sabit yağı, limon (Citrus limon) ve sardunya (Pelargonium graveolens) uçucu yağlarının antibakteriyel aktivitesi karşı agar kuyu difüzyon ve mikrodilüsyon yöntemiyle in vitro olarak değerlendirilmiştir. Test edilen Pseudomonas türlerine deve dikeni sabit yağı, limon ve sardunya uçucu yağlarının inhibisyon zonu oluşturmadığı ve MİK değerlerinin >80 μL/mL olduğu tespit edilmiştir.

🔖

Cite This Article

Ateş, G. Ö., Karli, R., Budak, F. (2025). Evaluation of Antibacterial Activity of Commercial Lemon, Geranium, and Thistle Oils Against Pseudomonas Species. Lapseki Meslek Yüksekokulu Uygulamalı Araştırmalar Dergisi, Cilt: 6 Sayı: 13, 1–9. https://ajindex.com/articles/1524469

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