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Journals / Ankara Üniversitesi Eczacılık Fakültesi Dergisi / 2026 / Cilt: 50 Sayı: 1

EVALUATION OF THE EFFECTS OF SULFAMETHOXAZOLE ON ENDOTHELIAL AND FIBROBLAST CELLS

SÜLFAMETOKSAZOLÜN ENDOTEL VE FİBROBLAST HÜCRELERİ ÜZERİNDEKİ ETKİLERİNİN DEĞERLENDİRİLMESİ

Makbule Beyza Şen(LOKMAN HEKİM ÜNİVERSİTESİ, ECZACILIK FAKÜLTESİ)0000-0003-4015-5595
Demet Kaçaroğlu(LOKMAN HEKİM ÜNİVERSİTESİ, TIP FAKÜLTESİ)0000-0003-4920-0516
Açelya Erikçi(Lokman Hekim Üniversitesi)0000-0001-9149-9510
Pages
106–113
Publication Date
2026-01-15
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

More detailed in vitro research is needed to expand the therapeutic areas in which SMX is used.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Objective: Sulfamethoxazole (SMX) is a sulfonamide derivative drug used in the treatment of bacterial infections.
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Key Findings & Data:Material and Method: In this study, HUVEC was used as endothelial cell line and L929 as fibroblast cell line.
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Conclusion & Impact:More detailed in vitro research is needed to expand the therapeutic areas in which SMX is used.
🔬Design:Araştırma Makalesi
📍Scope:Comprehensive Sample
⚙️Variables:Endotelyal hücre · fibroblast hücresi · sülfametoksazol

Abstract

Objective: Sulfamethoxazole (SMX) is a sulfonamide derivative drug used in the treatment of bacterial infections. The potential role of SMX not only in the treatment of infections but also in tissue regeneration and wound healing has aroused interest in its potential therapeutic applications. In this study, we aimed to determine the effects of SMX on fibroblast and endothelial cell viability, migration and collagen gene expression. Material and Method: In this study, HUVEC was used as endothelial cell line and L929 as fibroblast cell line. Cell viability was evaluated by MTT assay, migration inhibition by wound healing assay and COL1A1 gene expression by qRT-PCR method. Result and Discussion: SMX was found to show some positive effects on endothelial and fibroblast cells to promote wound healing, but some concentrations may negatively affect cellular processes. More detailed in vitro research is needed to expand the therapeutic areas in which SMX is used.

Özet

Amaç: Sülfametoksazol (SMX), bakteriyel enfeksiyonların tedavisinde kullanılan bir sülfonamid türevi ilaçtır. SMX sadece enfeksiyon tedavisinde değil, aynı zamanda doku rejenerasyonu ve yara iyileşmesindeki olası rolüyle de potansiyel terapötik uygulama alanları ilgi uyandırmaktadır. Bu çalışmada SMX’in fibroblast ve endotel hücrelerinin canlılığı, migrasyonu ve kollajen gen ekspresyonu üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu çalışmada, endotel hücre hattı olarak HUVEC ve fibroblast hücre hattı olarak ise L929 kullanıldı. Hücre canlılığı MTT testi ile, migrasyon inhibisyonu yara iyileşme testi ile ve COL1A1 gen ekspresyonu ise qRT-PCR yöntemi ile değerlendirildi. Sonuç ve Tartışma: SMX’in, endotel ve fibroblast hücrelerinde yara iyileşmesini destekleyecek bazı olumlu etkiler gösterdiği ancak bazı konsantrasyonların hücresel süreçleri olumsuz etkileyebileceği bulundu. SMX’in kullanıldığı terapötik alanlarının genişletilebilmesi için daha ayrıntılı in vitro araştırmalara ihtiyaç duyulmaktadır.

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Cite This Article

Şen, M. B., Kaçaroğlu, D., Erikçi, A. (2026). EVALUATION OF THE EFFECTS OF SULFAMETHOXAZOLE ON ENDOTHELIAL AND FIBROBLAST CELLS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, Cilt: 50 Sayı: 1, 106–113. https://doi.org/10.33483/jfpau.1661024

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