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Dergiler / İstanbul Journal of Pharmacy / 2023 / Cilt: 53 Sayı: 2

Antioxidant, cytotoxic and anti-inflammatory properties of Anthemis tricolor Boiss. through a series of cellular assays and inhibition of Turkish Macrovipera lebetina obtusa venom induced inflammation in rat

Dilara AŞKIN
Tuğçe DEMİRÖZ AKBULUT
Sura BAYKAN
Bayram GÖÇMEN
Shabana KHAN
Petek BALLAR KIRMIZIBAYRAK
Ayşe NALBANTSOY
Sayfa
166–176
Yayın Tarihi
2023-08-30
DOI
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⚡ TL;DR(1 Cümlede Hızlı Çıkarım)

The plant exhibits strong potential for treating local tissue damage in snake bites.

📌 3 Maddede Bu Makale

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Amaç & Hipotez:Background and Aims: Venom of Macrovipera lebetina obtusa (Viperidae), Turkey’s biggest viper, is highly toxic to humans and causes inflammation.
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Temel Bulgular & Veri:Methods: Antioxidant and anti-inflammatory activities were evaluated by DCFH-DA, iNOS, NF-κB inhibitions and NAG-1 gene activation, while WST-1 assay was preferred for cytotoxic activity.
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Sonuç & Katkı:The plant exhibits strong potential for treating local tissue damage in snake bites.
🔬Tür:Araştırma Makalesi
📍Kapsam:Genişletilmiş Akademik Örneklem
⚙️Değişkenler:Macrovipera lebetina obtusa · Anthemis tricolor · cytotoxicity

Abstract

Background and Aims: Venom of Macrovipera lebetina obtusa (Viperidae), Turkey’s biggest viper, is highly toxic to humans and causes inflammation. In Anatolian traditional medicine, many Anthemis L. species are used in wound healing and against inflammatory diseases. In the present study, antioxidant, cytotoxic and anti-inflammatory activities of different endemic A. tricolor Boiss. extracts were evaluated by in vitro assays. Besides, the anti-inflammatory activities of chloroform extract against carrageenan and snake venom induced-edema were investigated in rats. Methods: Antioxidant and anti-inflammatory activities were evaluated by DCFH-DA, iNOS, NF-κB inhibitions and NAG-1 gene activation, while WST-1 assay was preferred for cytotoxic activity. Besides, the hind paw edema test was used for in vivo studies. Results: Chloroform extract exhibited the strongest NAG-1-inducing activity. In addition, this extract showed potent iNOS and NF-κB inhibition (IC50:14.0 and 10.75 µg/mL, respectively) and cytotoxic effect against human osteosarcoma (U2OS; IC50: 15.18 µg/mL) and human cervical cancer (HeLa; IC50: 18.3 µg/mL) cell lines. Moreover, chloroform extract had stronger anti-inflammatory effects against both carrageenan and snake venom induced-edema formation than indomethacin. Fifty and 100 mg/kg extracts reduced the paw edema to 1-3% at 4 h after the snake venom injection. On the other hand, 50, 25 and 12.5 mg/kg of the extract completely inhibited inflammation induced by carrageenan. Conclusion: This is the first report on the antioxidant, cytotoxic and anti-inflammatory effects of endemic A. tricolor by in vitro studies and snake venom-induced paw edema in rats. The plant exhibits strong potential for treating local tissue damage in snake bites.

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Bu Makaleye Atıf Yap

AŞKIN, D., AKBULUT, T. D., BAYKAN, S., GÖÇMEN, B., KHAN, S., KIRMIZIBAYRAK, P. B., NALBANTSOY, A. (2023). Antioxidant, cytotoxic and anti-inflammatory properties of Anthemis tricolor Boiss. through a series of cellular assays and inhibition of Turkish Macrovipera lebetina obtusa venom induced inflammation in rat. İstanbul Journal of Pharmacy, Cilt: 53 Sayı: 2, 166–176. https://ajindex.com/articles/963641

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  1. Acquaviva, R., Giacomo, C. Di, Malaguarnera, M., Ragusa, S., San-tangelo, R., Mastrojeni, S. Doria, V. A. (2012). Biological activi-ties of extract of Anthemis aetnensis Schouw: In vitro evaluation. Journal of Medicinal Plants Research, 6(10), 1919-1925. https://doi. org/10.5897/JMPR11.1567 google scholar Al-Snafi, A. E. (2016). Medical importance of Anthemis nobilis (Chamaemelum nobile)-A review. Asian Journal of Pharmaceutical Science & Technology, 6(2), 89-95. google scholar Alexieva, B., Markova, T., Nikolova, E., Aragane, Y., & Higashino, H. (2010). Free radicals, antioxidants and cancer chemotherapy. Acta Medica Kinki University, 35(2), 57-65. google scholar Baek, S. J., Wilson, L. C., Lee, C. H., & Eling, T. E. (2002). Dual function of nonsteroidal anti-inflammatory drugs (NSAIDs): Inhibition of cyclooxygenase and induction of NSAID-activated gene. Journal of Pharmacology and Experimental Therapeutics, 301, 1126-1131. google scholar Baltaci, S., Kolatan, H. E., Yilmaz, O., & Kivçak, B. (2011). Anti-inflamma-tory activity of Anthemis aciphylla var. aciphylla Boiss. Turkish Journal of Biology, 35, 757-762. https://doi.org/10.3906/biy-1006-15 google scholar Baytop, T. (1999). Türkiye’de bitkilerle tedavi (geçmişte ve bugün) (2nd edn.). Istanbul, Turkey: Nobel Tıp Kitabevleri. google scholar Boukhary, R., Aboul-ElA, M., & El-Lakany, A. (2019). Review on chemical constituents and biological activities of genus An-themis. Pharmacognosy Journal, 11(5), 1155-1166. https://doi. org/10.5530/pj.2019.11.180 google scholar Bruno, M., Maggio, A., Arnold, A. N., Diaz, J. G., & Herz, W. (1998). Sesquiterpene lactones from Anthemis plutonia. Phytochemistry, 49, 1739-1740. google scholar Cakilcioglu, U., Khatun, S., Turkoglu, I., & Hayta, S. (2011). Ethno-pharmacological survey of medicinal plants in Maden (Elazig-Turkey). Journal of Ethnopharmacology, 137, 469-486. google scholar Chemsa, A. E., Zellagui, A., Öztürk, M., Erol, E., Ceylan, O., Duru, M. E., & Lahouel, M. (2018). Chemical composition, antioxidant, anticholinesterase, antimicrobial and antibiofilm activities of es-sential oil and methanolic extract of Anthemis stiparum subsp. sabulicola (Pomel) Oberpr. Microbial Pathogenesis, 119(February), 233-240. https://doi.org/10.1016/j.micpath.2018.04.033 google scholar Chippaux, J. P. (1998). Snake-bites: appraisal of the global situa-tion. Bulletin of the World Health Organization, 76(5), 515-524. google scholar Chippaux, J. P., Williams, V., & White, J. (199