Melatonin Decreases the Morphological Changes in Liver Induced by Magnetic Field Exposure in Rats

Özet

SüleymanDemirel Üniversitesi TIP FAKÜLTESİ DERGİSİ: 2000 Mart; 7(1) Melatonin Decreases the Morphological Changes in Liver Induced by Magnetic Field Exposure in Rats Alpaslan Gökçimen Abstract Melatonin decreases the morphological changes in liver induced by magnetic field exposure in rats. Aim: The purpose of this study was to investigate the possible effect ofmelatonin (m) on morphological changes induced by magnetic fields exposure in rat liver. Methods: Thirty young male wistar albino rats were used. Rats divided into 3 groups. First group (control group) received daily intra-peritoneal injections of saline for two weeks. Second group was magnetic field (MF) exposed to rats (n:10). Exposure was 27.12 MHz magnetic field energy, 2.5 hours/day for two weeks. Third group (MF+m) was exposed magnetic fields and had intra-peritoneal single dose of 4 mg/kg/ daily melatonin for two weeks. The liver tissue samples were taken under ether anesthesia at the end of the experiment and fixed in 10 % formalin. The liver samples blocked in paraffin after routin methods. Paraffin sections were dyed with hematoxylen-eosin and examined under 'Olympus BX50' light microscopy. Results: In histological examinations, mixed cell infiltrations noticed in the periportal area, a high enlargement in the portal area sinusoidal dilatations, granular and vacua far degenerations in the cytoplasm of the liver parenchyma were observed in MF group. The severity of these lesions in the MF+m group was lower than that in the MF group. Conclusion: Melatonin partially reduces the histo-pathologic effect of magnetic field on liver tissue in rats. Key words: magnetic field, melatonin, liver, morphological changes.